Lesser panda anellovirus
Average proteome isoelectric point is 7.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z2L0X1|A0A2Z2L0X1_9VIRU Capsid protein OS=Lesser panda anellovirus OX=2012640 PE=3 SV=1
MM1 pKa = 8.14 VEE3 pKa = 5.05 FILPYY8 pKa = 8.34 GQSRR12 pKa = 11.84 RR13 pKa = 11.84 SCKK16 pKa = 9.79 RR17 pKa = 11.84 SRR19 pKa = 11.84 FDD21 pKa = 3.68 AVGGCRR27 pKa = 11.84 RR28 pKa = 11.84 TSGACPEE35 pKa = 4.04 SRR37 pKa = 11.84 GLSGKK42 pKa = 10.47 GEE44 pKa = 4.0 YY45 pKa = 9.95 RR46 pKa = 11.84 QMGSPEE52 pKa = 4.2 GQSADD57 pKa = 3.46 AGHH60 pKa = 6.58 SEE62 pKa = 4.22 TLWLEE67 pKa = 4.17 SCLLTHH73 pKa = 7.18 SLWCKK78 pKa = 9.29 CNDD81 pKa = 2.83 WRR83 pKa = 11.84 EE84 pKa = 4.27 HH85 pKa = 4.64 VRR87 pKa = 11.84 KK88 pKa = 8.24 TDD90 pKa = 3.47 STWRR94 pKa = 11.84 SSTGGGDD101 pKa = 3.17 RR102 pKa = 11.84 TGEE105 pKa = 3.95 EE106 pKa = 4.21 GGVPDD111 pKa = 4.26 EE112 pKa = 4.52 VAVHH116 pKa = 6.36 FDD118 pKa = 3.47 LGFSEE123 pKa = 5.38 EE124 pKa = 4.47 DD125 pKa = 3.22 DD126 pKa = 3.68 TRR128 pKa = 11.84 TGEE131 pKa = 3.97 RR132 pKa = 3.67
Molecular weight: 14.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.396
IPC2_protein 5.461
IPC_protein 5.397
Toseland 5.537
ProMoST 5.626
Dawson 5.512
Bjellqvist 5.575
Wikipedia 5.436
Rodwell 5.461
Grimsley 5.55
Solomon 5.512
Lehninger 5.474
Nozaki 5.703
DTASelect 5.855
Thurlkill 5.703
EMBOSS 5.652
Sillero 5.779
Patrickios 4.164
IPC_peptide 5.525
IPC2_peptide 5.792
IPC2.peptide.svr19 5.816
Protein with the highest isoelectric point:
>tr|A0A2Z2L314|A0A2Z2L314_9VIRU ORF3 OS=Lesser panda anellovirus OX=2012640 PE=4 SV=1
MM1 pKa = 7.48 AFFYY5 pKa = 10.77 RR6 pKa = 11.84 RR7 pKa = 11.84 WRR9 pKa = 11.84 PYY11 pKa = 8.07 WRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 WCPRR19 pKa = 11.84 RR20 pKa = 11.84 GGRR23 pKa = 11.84 SFRR26 pKa = 11.84 PRR28 pKa = 11.84 IFRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 YY35 pKa = 7.02 TYY37 pKa = 8.52 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.04 VRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 PRR51 pKa = 11.84 RR52 pKa = 11.84 VRR54 pKa = 11.84 PKK56 pKa = 10.48 KK57 pKa = 9.97 IIQWQPQHH65 pKa = 5.24 QAKK68 pKa = 10.36 CIIRR72 pKa = 11.84 GWFPAMWAHH81 pKa = 6.13 SAFVTYY87 pKa = 10.13 PMYY90 pKa = 10.86 KK91 pKa = 9.76 WIPAKK96 pKa = 10.21 QGEE99 pKa = 4.48 YY100 pKa = 10.57 GHH102 pKa = 6.68 FFTMEE107 pKa = 3.84 GCVTLMHH114 pKa = 6.36 FTLGLLYY121 pKa = 10.57 KK122 pKa = 10.12 EE123 pKa = 4.07 WLKK126 pKa = 10.19 MRR128 pKa = 11.84 NTWSRR133 pKa = 11.84 SNDD136 pKa = 3.06 GFDD139 pKa = 3.14 LAKK142 pKa = 10.9 YY143 pKa = 10.28 SGTKK147 pKa = 8.84 LTFRR151 pKa = 11.84 PHH153 pKa = 6.56 PFVSYY158 pKa = 8.32 MVWWEE163 pKa = 3.99 RR164 pKa = 11.84 NFGEE168 pKa = 4.26 VHH170 pKa = 6.92 PIQYY174 pKa = 10.21 RR175 pKa = 11.84 SIHH178 pKa = 6.34 PAVIMHH184 pKa = 5.96 QPNHH188 pKa = 6.17 IFVPSLLVNPXGRR201 pKa = 11.84 KK202 pKa = 8.17 RR203 pKa = 11.84 KK204 pKa = 9.24 VWIPPPSTLTSTWSFMKK221 pKa = 9.83 YY222 pKa = 5.73 WCEE225 pKa = 3.48 VALFRR230 pKa = 11.84 VGITPINLKK239 pKa = 9.97 SQFLHH244 pKa = 6.51 IGTVSTGWWIGYY256 pKa = 7.31 QPKK259 pKa = 10.56 NNYY262 pKa = 8.01 NHH264 pKa = 6.6 FSDD267 pKa = 5.37 PDD269 pKa = 3.66 MLPQMNSEE277 pKa = 4.28 QYY279 pKa = 10.78 CVGEE283 pKa = 4.24 GNKK286 pKa = 9.33 IFYY289 pKa = 9.2 KK290 pKa = 9.77 WWWDD294 pKa = 3.56 DD295 pKa = 3.32 GTDD298 pKa = 3.86 NKK300 pKa = 10.1 WGAKK304 pKa = 9.31 NRR306 pKa = 11.84 TDD308 pKa = 3.39 QYY310 pKa = 11.13 NNEE313 pKa = 4.34 TGVLPPVAQKK323 pKa = 9.93 TSTDD327 pKa = 3.47 NIVPINVPYY336 pKa = 10.21 WLAFWGAGTTFLEE349 pKa = 4.02 WDD351 pKa = 4.02 KK352 pKa = 11.66 NDD354 pKa = 4.38 LYY356 pKa = 10.79 IWWYY360 pKa = 10.69 YY361 pKa = 11.06 DD362 pKa = 4.28 DD363 pKa = 4.45 NSKK366 pKa = 9.75 TNIDD370 pKa = 4.17 EE371 pKa = 4.71 LDD373 pKa = 3.5 GAAKK377 pKa = 9.47 KK378 pKa = 10.34 KK379 pKa = 9.95 RR380 pKa = 11.84 WINVSCGQMLNGIGIQNIIQGGPFVLNPNDD410 pKa = 3.6 IFPEE414 pKa = 4.11 KK415 pKa = 10.59 NINLSFWYY423 pKa = 10.16 QSFWRR428 pKa = 11.84 WGGISPSPPINVDD441 pKa = 3.15 PCQVDD446 pKa = 3.67 PAHH449 pKa = 6.57 APQRR453 pKa = 11.84 RR454 pKa = 11.84 RR455 pKa = 11.84 VPRR458 pKa = 11.84 GVPEE462 pKa = 4.28 SPKK465 pKa = 10.71 HH466 pKa = 5.83 VGDD469 pKa = 3.84 WSLHH473 pKa = 5.26 PWDD476 pKa = 4.6 LTRR479 pKa = 11.84 QGLITEE485 pKa = 5.01 AKK487 pKa = 9.44 LKK489 pKa = 10.55 QIFGFSPDD497 pKa = 4.48 DD498 pKa = 3.5 IAGLPHH504 pKa = 6.7 PPSRR508 pKa = 11.84 EE509 pKa = 3.97 SSPATTDD516 pKa = 3.0 SSPPPTQLRR525 pKa = 11.84 AVRR528 pKa = 11.84 RR529 pKa = 11.84 KK530 pKa = 9.63 RR531 pKa = 11.84 SRR533 pKa = 11.84 SEE535 pKa = 5.03 DD536 pKa = 2.91 LWQSPDD542 pKa = 3.39 SEE544 pKa = 4.41 EE545 pKa = 4.65 EE546 pKa = 4.06 KK547 pKa = 11.03 VQTGDD552 pKa = 3.49 TLSLLAQRR560 pKa = 11.84 IEE562 pKa = 3.89 RR563 pKa = 11.84 EE564 pKa = 3.11 RR565 pKa = 11.84 DD566 pKa = 3.18 FRR568 pKa = 11.84 HH569 pKa = 6.55 KK570 pKa = 10.12 LRR572 pKa = 11.84 KK573 pKa = 9.58 RR574 pKa = 11.84 IRR576 pKa = 11.84 DD577 pKa = 3.31 WLTT580 pKa = 2.8
Molecular weight: 68.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.589
IPC_protein 10.058
Toseland 10.335
ProMoST 10.028
Dawson 10.496
Bjellqvist 10.189
Wikipedia 10.687
Rodwell 10.804
Grimsley 10.57
Solomon 10.54
Lehninger 10.511
Nozaki 10.335
DTASelect 10.189
Thurlkill 10.365
EMBOSS 10.73
Sillero 10.423
Patrickios 10.423
IPC_peptide 10.54
IPC2_peptide 9.048
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
974
132
580
324.7
37.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.209 ± 0.449
2.156 ± 0.919
4.928 ± 0.533
5.749 ± 1.933
3.799 ± 0.909
8.316 ± 2.045
2.669 ± 0.157
3.799 ± 1.821
5.031 ± 0.687
5.955 ± 0.081
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.745 ± 0.286
3.388 ± 1.297
6.982 ± 1.455
3.491 ± 0.724
10.575 ± 0.535
9.035 ± 2.647
6.263 ± 0.976
4.415 ± 0.402
4.312 ± 1.21
3.08 ± 0.665
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here