Arthrobacter sp. NCCP-1664
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5A7NS01|A0A5A7NS01_9MICC Molybdopterin molybdenumtransferase OS=Arthrobacter sp. NCCP-1664 OX=1682741 GN=NCCP1664_10180 PE=3 SV=1
MM1 pKa = 7.29 PTITYY6 pKa = 8.76 IQPDD10 pKa = 3.9 GTAEE14 pKa = 3.84 AMTVEE19 pKa = 5.14 DD20 pKa = 4.24 GTTLMRR26 pKa = 11.84 AAVTSGVDD34 pKa = 4.14 GIVGEE39 pKa = 4.64 CGGQAMCATCHH50 pKa = 5.7 VYY52 pKa = 10.17 VRR54 pKa = 11.84 PEE56 pKa = 3.5 FAGRR60 pKa = 11.84 LPQIGDD66 pKa = 4.43 DD67 pKa = 3.87 EE68 pKa = 6.64 DD69 pKa = 4.68 EE70 pKa = 4.62 MLDD73 pKa = 3.53 CTTAEE78 pKa = 3.92 RR79 pKa = 11.84 TDD81 pKa = 3.54 RR82 pKa = 11.84 SRR84 pKa = 11.84 LGCQISVDD92 pKa = 3.67 AGLDD96 pKa = 3.97 GIVVDD101 pKa = 5.08 VPADD105 pKa = 3.5 QVV107 pKa = 3.26
Molecular weight: 11.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.859
IPC_protein 3.821
Toseland 3.605
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.757
Nozaki 3.948
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.948
Patrickios 1.901
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A5A7NRC3|A0A5A7NRC3_9MICC DNA invertase OS=Arthrobacter sp. NCCP-1664 OX=1682741 GN=NCCP1664_17650 PE=3 SV=1
MM1 pKa = 7.41 GLSNIIRR8 pKa = 11.84 NAAEE12 pKa = 4.03 RR13 pKa = 11.84 FTGRR17 pKa = 11.84 TTGTTSGRR25 pKa = 11.84 TGTGGLGTSRR35 pKa = 11.84 GTGTGRR41 pKa = 11.84 LGGGGIGGTIRR52 pKa = 11.84 NLLRR56 pKa = 11.84 RR57 pKa = 11.84 RR58 pKa = 3.64
Molecular weight: 5.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.384
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.115
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.857
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2960
2
2962
963181
39
1788
325.2
34.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.235 ± 0.075
0.629 ± 0.012
5.316 ± 0.034
5.886 ± 0.039
3.02 ± 0.028
9.766 ± 0.044
2.149 ± 0.023
3.746 ± 0.03
2.238 ± 0.031
10.407 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.9 ± 0.019
2.112 ± 0.025
5.878 ± 0.042
2.88 ± 0.025
7.365 ± 0.047
5.24 ± 0.035
5.509 ± 0.037
8.377 ± 0.034
1.385 ± 0.018
1.962 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here