Enterobacter phage vB_EhoM-IME523

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Kanagawavirus; unclassified Kanagawavirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 289 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G3KED7|A0A7G3KED7_9CAUD Uncharacterized protein OS=Enterobacter phage vB_EhoM-IME523 OX=2596709 PE=4 SV=1
MM1 pKa = 7.2AQVIINGSQKK11 pKa = 10.64AIEE14 pKa = 4.57EE15 pKa = 4.25FCSWFSNSGEE25 pKa = 4.1QGFMEE30 pKa = 4.89AWADD34 pKa = 3.71SGWNAKK40 pKa = 7.28EE41 pKa = 4.17CKK43 pKa = 9.68YY44 pKa = 11.44DD45 pKa = 4.72DD46 pKa = 3.36VTTYY50 pKa = 10.4IGTRR54 pKa = 11.84GYY56 pKa = 10.68GINEE60 pKa = 4.58PIEE63 pKa = 3.82LVEE66 pKa = 4.04YY67 pKa = 10.77DD68 pKa = 3.97KK69 pKa = 11.17EE70 pKa = 4.34TDD72 pKa = 3.45EE73 pKa = 4.25EE74 pKa = 4.62VPYY77 pKa = 11.24VDD79 pKa = 4.54

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G3KCS1|A0A7G3KCS1_9CAUD Uncharacterized protein OS=Enterobacter phage vB_EhoM-IME523 OX=2596709 PE=4 SV=1
MM1 pKa = 7.9PCRR4 pKa = 11.84KK5 pKa = 9.71VGLINKK11 pKa = 8.96RR12 pKa = 11.84PKK14 pKa = 10.01PSEE17 pKa = 3.26EE18 pKa = 3.76RR19 pKa = 11.84MRR21 pKa = 11.84KK22 pKa = 8.11FQEE25 pKa = 4.58FINAALKK32 pKa = 10.56RR33 pKa = 11.84NKK35 pKa = 10.08

Molecular weight: 4.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

289

0

289

53879

31

1239

186.4

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.289 ± 0.16

1.093 ± 0.062

6.405 ± 0.107

7.27 ± 0.174

4.475 ± 0.124

6.338 ± 0.155

1.787 ± 0.076

6.693 ± 0.106

7.227 ± 0.183

7.53 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.988 ± 0.088

5.132 ± 0.128

3.727 ± 0.094

3.662 ± 0.097

4.508 ± 0.089

5.887 ± 0.122

5.609 ± 0.162

6.858 ± 0.115

1.483 ± 0.059

4.039 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski