Calypte anna (Anna s hummingbird) (Archilochus anna)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia;

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091I092|A0A091I092_CALAN tRNA-splicing endonuclease subunit Sen15 (Fragment) OS=Calypte anna OX=9244 GN=N300_02679 PE=3 SV=1
SS1 pKa = 6.72RR2 pKa = 11.84LSWLEE7 pKa = 3.51NSGVGFSLDD16 pKa = 3.46YY17 pKa = 9.24PTISLHH23 pKa = 6.16AVSRR27 pKa = 11.84DD28 pKa = 3.12LTAYY32 pKa = 9.12PCEE35 pKa = 4.05HH36 pKa = 7.04LYY38 pKa = 11.68VMVNAKK44 pKa = 10.45FEE46 pKa = 4.19GEE48 pKa = 4.21EE49 pKa = 4.05TKK51 pKa = 10.37EE52 pKa = 4.0APVAEE57 pKa = 4.54GEE59 pKa = 4.22EE60 pKa = 4.28EE61 pKa = 4.65EE62 pKa = 5.01EE63 pKa = 5.64DD64 pKa = 3.85SDD66 pKa = 6.06DD67 pKa = 3.93DD68 pKa = 4.44AEE70 pKa = 5.13PIAEE74 pKa = 4.02FRR76 pKa = 11.84FVPSDD81 pKa = 3.36KK82 pKa = 10.88SALEE86 pKa = 3.9AMFSAMCEE94 pKa = 4.27CQALHH99 pKa = 7.41PDD101 pKa = 4.21PDD103 pKa = 5.77DD104 pKa = 4.05EE105 pKa = 7.61DD106 pKa = 4.46SDD108 pKa = 3.89NDD110 pKa = 3.96YY111 pKa = 11.58EE112 pKa = 4.77GDD114 pKa = 3.7EE115 pKa = 4.54YY116 pKa = 11.44DD117 pKa = 4.51VEE119 pKa = 4.31AHH121 pKa = 6.63EE122 pKa = 5.44LGQGDD127 pKa = 3.72IPTFYY132 pKa = 10.0TYY134 pKa = 11.09EE135 pKa = 4.17EE136 pKa = 4.62GLSHH140 pKa = 6.9LTAEE144 pKa = 4.71GQATLEE150 pKa = 4.19RR151 pKa = 11.84LEE153 pKa = 4.57GMLGHH158 pKa = 6.93TGCCFSDD165 pKa = 3.41GMEE168 pKa = 3.81VDD170 pKa = 3.44IAPVVAGQFEE180 pKa = 4.42DD181 pKa = 5.26AEE183 pKa = 4.32VDD185 pKa = 3.59HH186 pKa = 7.08

Molecular weight: 20.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091HM96|A0A091HM96_CALAN TBC domain-containing protein kinase-like OS=Calypte anna OX=9244 GN=N300_08664 PE=4 SV=1
KKK2 pKa = 10.12RR3 pKa = 11.84QGKKK7 pKa = 8.85RR8 pKa = 11.84QGKKK12 pKa = 8.73RR13 pKa = 11.84QGKKK17 pKa = 8.73RR18 pKa = 11.84QGKKK22 pKa = 8.73RR23 pKa = 11.84QGKKK27 pKa = 8.73RR28 pKa = 11.84QGKKK32 pKa = 8.73RR33 pKa = 11.84QGKKK37 pKa = 8.73RR38 pKa = 11.84QGKKK42 pKa = 8.73RR43 pKa = 11.84QGKKK47 pKa = 8.73RR48 pKa = 11.84QGKKK52 pKa = 8.73RR53 pKa = 11.84QGKKK57 pKa = 9.07RR58 pKa = 11.84

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13267

0

13267

5940916

32

6204

447.8

50.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.396 ± 0.019

2.262 ± 0.018

4.969 ± 0.016

7.206 ± 0.029

3.9 ± 0.017

5.999 ± 0.031

2.574 ± 0.01

4.924 ± 0.014

6.379 ± 0.024

9.805 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.008

4.045 ± 0.015

5.371 ± 0.026

4.721 ± 0.02

5.247 ± 0.017

8.198 ± 0.028

5.351 ± 0.016

6.256 ± 0.015

1.21 ± 0.008

2.964 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski