Arachidicoccus sp. BS20
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2946 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191TMC5|A0A191TMC5_9BACT Phosphonate ABC transporter ATP-binding protein OS=Arachidicoccus sp. BS20 OX=1850526 GN=A9P82_11625 PE=4 SV=1
MM1 pKa = 7.7 ANWCNNTVVFEE12 pKa = 4.48 GKK14 pKa = 9.71 PEE16 pKa = 5.0 AIRR19 pKa = 11.84 QIQQLFKK26 pKa = 11.18 EE27 pKa = 4.47 MAEE30 pKa = 4.02 QEE32 pKa = 4.29 QKK34 pKa = 10.61 EE35 pKa = 4.76 GCGQLPDD42 pKa = 5.15 FVADD46 pKa = 3.64 SNGGYY51 pKa = 10.47 FFGIYY56 pKa = 9.34 QDD58 pKa = 3.87 YY59 pKa = 11.29 DD60 pKa = 3.41 NTDD63 pKa = 2.97 TFQYY67 pKa = 6.7 EE68 pKa = 4.89 TKK70 pKa = 9.52 WSPNMEE76 pKa = 4.29 VLQKK80 pKa = 10.32 IAEE83 pKa = 4.24 HH84 pKa = 6.04 YY85 pKa = 10.57 KK86 pKa = 10.03 VDD88 pKa = 4.19 FTQDD92 pKa = 3.55 YY93 pKa = 10.41 EE94 pKa = 4.33 EE95 pKa = 4.99 LGCLVFGRR103 pKa = 11.84 ATFSDD108 pKa = 3.79 RR109 pKa = 11.84 LLTDD113 pKa = 3.62 IYY115 pKa = 11.06 LDD117 pKa = 5.08 DD118 pKa = 4.52 EE119 pKa = 5.74 DD120 pKa = 4.6 FDD122 pKa = 4.2 KK123 pKa = 11.78 YY124 pKa = 11.15 EE125 pKa = 4.08 YY126 pKa = 10.8 DD127 pKa = 3.61 EE128 pKa = 5.2 EE129 pKa = 4.82 NSVWYY134 pKa = 10.16 FEE136 pKa = 4.28 GEE138 pKa = 4.41 TYY140 pKa = 10.66 EE141 pKa = 3.95 SDD143 pKa = 4.03 YY144 pKa = 11.37 EE145 pKa = 3.93 ILEE148 pKa = 4.06 ILLEE152 pKa = 4.11 RR153 pKa = 11.84 KK154 pKa = 9.32 IEE156 pKa = 4.02 NHH158 pKa = 6.4 HH159 pKa = 6.32 PP160 pKa = 3.59
Molecular weight: 19.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.839
IPC2_protein 4.062
IPC_protein 4.012
Toseland 3.834
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.846
Rodwell 3.846
Grimsley 3.745
Solomon 3.961
Lehninger 3.923
Nozaki 4.075
DTASelect 4.24
Thurlkill 3.859
EMBOSS 3.872
Sillero 4.126
Patrickios 0.896
IPC_peptide 3.973
IPC2_peptide 4.113
IPC2.peptide.svr19 3.999
Protein with the highest isoelectric point:
>tr|A0A191THK6|A0A191THK6_9BACT ABC transporter OS=Arachidicoccus sp. BS20 OX=1850526 GN=A9P82_02035 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.31 KK3 pKa = 10.46 VILSLIVLIAATGLHH18 pKa = 5.98 AQTVVVIHH26 pKa = 6.65 RR27 pKa = 11.84 PVIVAPRR34 pKa = 11.84 VAVKK38 pKa = 9.74 MGPVVATTPLPAPVVVAPRR57 pKa = 11.84 PAVVVKK63 pKa = 9.08 PAVVVAPKK71 pKa = 9.85 PVVVRR76 pKa = 11.84 PIVHH80 pKa = 6.09 PVVVARR86 pKa = 11.84 PVIVHH91 pKa = 6.58 PAPVIVRR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 TVVVRR105 pKa = 11.84 RR106 pKa = 11.84 VYY108 pKa = 10.96
Molecular weight: 11.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2946
0
2946
1021605
38
2443
346.8
39.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.123 ± 0.033
0.798 ± 0.013
5.391 ± 0.029
5.598 ± 0.044
5.111 ± 0.031
6.393 ± 0.044
1.92 ± 0.02
7.541 ± 0.041
7.408 ± 0.045
9.178 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.181 ± 0.019
6.309 ± 0.053
3.6 ± 0.023
3.846 ± 0.026
3.569 ± 0.026
6.605 ± 0.037
5.686 ± 0.038
6.11 ± 0.027
1.234 ± 0.017
4.397 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here