Mus musculus papillomavirus type 1
Average proteome isoelectric point is 5.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9D5K4|D9D5K4_9PAPI Replication protein E1 OS=Mus musculus papillomavirus type 1 OX=763552 GN=E1 PE=2 SV=1
MM1 pKa = 7.28 QGPLPTIADD10 pKa = 3.52 IEE12 pKa = 4.28 IQNLDD17 pKa = 3.37 SLLGVGEE24 pKa = 4.56 PDD26 pKa = 3.68 LPDD29 pKa = 3.57 VGSSSLSPDD38 pKa = 3.06 SLGEE42 pKa = 4.0 EE43 pKa = 4.33 EE44 pKa = 4.5 EE45 pKa = 5.15 LEE47 pKa = 4.56 LEE49 pKa = 4.64 TIDD52 pKa = 3.96 VDD54 pKa = 3.81 PYY56 pKa = 10.76 RR57 pKa = 11.84 IKK59 pKa = 8.15 TTCFCCDD66 pKa = 2.86 TVLRR70 pKa = 11.84 FIIVTGDD77 pKa = 3.53 DD78 pKa = 3.29 SVKK81 pKa = 10.72 AFEE84 pKa = 4.86 SLLLQDD90 pKa = 4.85 LSFVCPHH97 pKa = 6.56 CVASYY102 pKa = 10.61 VNLRR106 pKa = 11.84 NGKK109 pKa = 9.08 RR110 pKa = 3.24
Molecular weight: 12.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.995
IPC2_protein 3.986
IPC_protein 3.923
Toseland 3.732
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.834
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.846
Nozaki 4.024
DTASelect 4.228
Thurlkill 3.783
EMBOSS 3.846
Sillero 4.05
Patrickios 2.905
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.938
Protein with the highest isoelectric point:
>tr|D9D5K3|D9D5K3_9PAPI Protein E7 OS=Mus musculus papillomavirus type 1 OX=763552 GN=E7 PE=2 SV=1
MM1 pKa = 7.92 EE2 pKa = 5.32 IGKK5 pKa = 10.41 GYY7 pKa = 7.81 TLEE10 pKa = 3.99 EE11 pKa = 3.9 VLRR14 pKa = 11.84 YY15 pKa = 9.76 SNKK18 pKa = 10.37 DD19 pKa = 3.09 VVDD22 pKa = 4.27 FHH24 pKa = 7.01 LSCAFCSTTMDD35 pKa = 3.6 HH36 pKa = 6.39 NEE38 pKa = 3.76 KK39 pKa = 10.85 ARR41 pKa = 11.84 FIQAKK46 pKa = 8.37 LKK48 pKa = 10.8 CVVRR52 pKa = 11.84 DD53 pKa = 3.89 FAFKK57 pKa = 10.36 GACIVCRR64 pKa = 11.84 RR65 pKa = 11.84 QLACKK70 pKa = 9.91 EE71 pKa = 4.24 KK72 pKa = 10.45 LLHH75 pKa = 5.84 TRR77 pKa = 11.84 VTGEE81 pKa = 3.85 ADD83 pKa = 3.82 LVEE86 pKa = 5.0 CMAGKK91 pKa = 10.3 NIVFVTVRR99 pKa = 11.84 CVTCLALLTASEE111 pKa = 4.33 KK112 pKa = 10.88 LDD114 pKa = 3.74 AKK116 pKa = 11.02 ACGLPFHH123 pKa = 6.97 LVRR126 pKa = 11.84 HH127 pKa = 5.58 MWRR130 pKa = 11.84 GYY132 pKa = 10.84 CGFCKK137 pKa = 10.18 PLLL140 pKa = 3.97
Molecular weight: 15.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.751
IPC2_protein 7.834
IPC_protein 7.644
Toseland 7.117
ProMoST 8.244
Dawson 8.346
Bjellqvist 8.858
Wikipedia 8.244
Rodwell 8.361
Grimsley 7.102
Solomon 8.419
Lehninger 8.448
Nozaki 9.253
DTASelect 8.478
Thurlkill 8.551
EMBOSS 8.565
Sillero 8.931
Patrickios 4.139
IPC_peptide 8.419
IPC2_peptide 8.126
IPC2.peptide.svr19 8.108
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2432
105
620
347.4
38.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.387 ± 0.691
2.097 ± 0.635
6.538 ± 0.443
5.921 ± 0.459
4.359 ± 0.478
7.237 ± 0.756
1.85 ± 0.269
4.4 ± 0.68
4.811 ± 0.773
8.758 ± 0.846
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.809 ± 0.336
4.77 ± 0.594
6.373 ± 0.985
4.071 ± 0.556
5.839 ± 0.281
7.936 ± 0.835
6.908 ± 0.345
6.661 ± 0.941
1.234 ± 0.361
3.043 ± 0.508
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here