Thalassotalea agarivorans (Thalassomonas agarivorans)
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2994 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I0BKS3|A0A1I0BKS3_THASX Hydrolase_4 domain-containing protein OS=Thalassotalea agarivorans OX=349064 GN=SAMN05660429_00982 PE=4 SV=1
MM1 pKa = 6.92 ITSSKK6 pKa = 10.42 KK7 pKa = 9.63 IVSAMLSTSLWIGVASTAFAEE28 pKa = 4.61 TTNVEE33 pKa = 3.97 AEE35 pKa = 4.64 GYY37 pKa = 7.94 STIGGTYY44 pKa = 9.73 QDD46 pKa = 5.21 GNPQPINIYY55 pKa = 10.0 SVNGVQAINFVNRR68 pKa = 11.84 GDD70 pKa = 3.52 FAEE73 pKa = 4.29 YY74 pKa = 10.33 DD75 pKa = 3.88 VSVSTAGEE83 pKa = 3.76 YY84 pKa = 10.53 SIEE87 pKa = 3.91 YY88 pKa = 10.52 LIGTSIASGSAIEE101 pKa = 4.53 ISVLVDD107 pKa = 3.95 GNWQSAGSTNVPLGHH122 pKa = 7.29 WDD124 pKa = 3.42 NFQALAATNNISLAQGTNRR143 pKa = 11.84 IKK145 pKa = 9.67 ITGAGTHH152 pKa = 6.15 DD153 pKa = 3.73 WQWNLDD159 pKa = 3.76 AFSLTLEE166 pKa = 4.4 TPEE169 pKa = 5.56 NPDD172 pKa = 4.35 NPDD175 pKa = 3.51 NPDD178 pKa = 3.52 NPDD181 pKa = 3.44 NPDD184 pKa = 3.49 NPDD187 pKa = 4.3 DD188 pKa = 4.94 GNTGQPGTPFTIEE201 pKa = 3.72 MEE203 pKa = 4.35 AFNATGSDD211 pKa = 3.68 DD212 pKa = 3.59 PRR214 pKa = 11.84 AQGMIIGEE222 pKa = 3.96 RR223 pKa = 11.84 GYY225 pKa = 10.03 PEE227 pKa = 5.41 DD228 pKa = 3.22 KK229 pKa = 10.14 HH230 pKa = 6.02 TVVDD234 pKa = 4.51 SNQTTDD240 pKa = 2.77 WVDD243 pKa = 3.27 YY244 pKa = 10.72 NINFPVSGNYY254 pKa = 9.55 RR255 pKa = 11.84 IEE257 pKa = 4.08 MLASGQTSHH266 pKa = 6.08 ATAILFVDD274 pKa = 3.81 NVQINEE280 pKa = 4.21 VPVDD284 pKa = 3.53 TGNQAVFQDD293 pKa = 4.62 FEE295 pKa = 4.75 LTDD298 pKa = 3.35 STYY301 pKa = 10.89 ISAGAHH307 pKa = 5.84 TIRR310 pKa = 11.84 VQSGSQINEE319 pKa = 4.07 FSWMWFGDD327 pKa = 3.49 ALTFTPLDD335 pKa = 4.03 GGSTDD340 pKa = 4.44 GDD342 pKa = 3.74 ADD344 pKa = 3.74 NDD346 pKa = 4.19 GVLDD350 pKa = 5.16 SVDD353 pKa = 3.52 TCPNTPAGAQVDD365 pKa = 4.07 ANGCEE370 pKa = 4.04 IIVDD374 pKa = 3.83 NDD376 pKa = 3.34 TDD378 pKa = 3.92 NDD380 pKa = 4.2 GVDD383 pKa = 3.88 NSIDD387 pKa = 3.52 QCPNTPAGAQVDD399 pKa = 4.09 ANGCEE404 pKa = 3.8 IVAVVDD410 pKa = 3.72 ADD412 pKa = 3.65 NDD414 pKa = 4.12 GVEE417 pKa = 5.21 DD418 pKa = 4.5 SLDD421 pKa = 3.53 MCPNTPAGAPVNGQGCADD439 pKa = 3.85 SQLDD443 pKa = 3.57 ADD445 pKa = 4.36 NDD447 pKa = 4.3 GVSDD451 pKa = 6.62 DD452 pKa = 4.87 IDD454 pKa = 3.62 QCPSTPAGSVVDD466 pKa = 3.91 GTGCIVVTPPADD478 pKa = 3.43 SDD480 pKa = 3.79 NDD482 pKa = 4.0 GVADD486 pKa = 4.21 TLDD489 pKa = 3.73 MCPNTATGLTVDD501 pKa = 3.9 SQGCALSQLDD511 pKa = 3.97 SDD513 pKa = 4.39 NDD515 pKa = 3.93 GVTDD519 pKa = 6.85 DD520 pKa = 5.44 IDD522 pKa = 3.48 QCANTPSGEE531 pKa = 4.19 TANATGCSSSQEE543 pKa = 4.21 GGGTDD548 pKa = 3.63 PGTPQPGLLYY558 pKa = 11.0 GEE560 pKa = 5.06 LAGAMNVSDD569 pKa = 4.71 TNPNWEE575 pKa = 4.16 RR576 pKa = 11.84 TTDD579 pKa = 3.84 LLQTEE584 pKa = 4.91 DD585 pKa = 4.07 SIKK588 pKa = 11.01 GNTTEE593 pKa = 4.2 VYY595 pKa = 9.97 TGFIYY600 pKa = 10.85 DD601 pKa = 3.74 ADD603 pKa = 3.87 GHH605 pKa = 6.51 ISFYY609 pKa = 10.14 EE610 pKa = 4.46 HH611 pKa = 6.94 IDD613 pKa = 3.45 DD614 pKa = 5.24 SVRR617 pKa = 11.84 LYY619 pKa = 10.53 IDD621 pKa = 3.54 GVLVLSNDD629 pKa = 2.74 SWEE632 pKa = 4.61 ASSQTTDD639 pKa = 3.62 LNLTPGTHH647 pKa = 6.53 EE648 pKa = 3.7 IEE650 pKa = 4.19 LRR652 pKa = 11.84 IGNADD657 pKa = 3.67 GGSGAVDD664 pKa = 4.42 GIGFGIDD671 pKa = 2.98 VDD673 pKa = 3.83 GGTNFVHH680 pKa = 7.4 PSTLSEE686 pKa = 4.58 SIFTSVGEE694 pKa = 4.19 EE695 pKa = 3.89 TGNPDD700 pKa = 4.41 LEE702 pKa = 4.37 QEE704 pKa = 3.85 GDD706 pKa = 3.6 IIVEE710 pKa = 4.05 LEE712 pKa = 4.17 SFVFTSTNGRR722 pKa = 11.84 VGGDD726 pKa = 3.35 SVEE729 pKa = 4.47 GFSPTATGVNWVTNGDD745 pKa = 3.69 YY746 pKa = 11.08 GDD748 pKa = 3.86 YY749 pKa = 9.75 MVTFEE754 pKa = 5.2 EE755 pKa = 4.89 PGTYY759 pKa = 9.38 SAYY762 pKa = 8.88 ITISAANDD770 pKa = 3.45 GSYY773 pKa = 10.64 GARR776 pKa = 11.84 VDD778 pKa = 3.47 VDD780 pKa = 3.51 GWPVAWGYY788 pKa = 11.05 FGGTGSWDD796 pKa = 3.32 VSSEE800 pKa = 3.57 NLLYY804 pKa = 10.94 GGTFVVEE811 pKa = 3.88 QAGEE815 pKa = 3.97 KK816 pKa = 9.34 VVRR819 pKa = 11.84 VEE821 pKa = 5.28 AIGGSDD827 pKa = 3.53 WQWSGDD833 pKa = 3.16 RR834 pKa = 11.84 VRR836 pKa = 11.84 FTRR839 pKa = 11.84 LGDD842 pKa = 3.31 VTAIPSPIYY851 pKa = 10.84 NPDD854 pKa = 3.21 DD855 pKa = 3.96 HH856 pKa = 7.09 FVAEE860 pKa = 4.25 IQGPQTDD867 pKa = 3.52 VTYY870 pKa = 10.65 LKK872 pKa = 10.73 KK873 pKa = 10.31 PVEE876 pKa = 3.9 IPANKK881 pKa = 9.53 KK882 pKa = 8.2 VLKK885 pKa = 10.35 SDD887 pKa = 2.47 VWYY890 pKa = 8.63 TYY892 pKa = 8.87 PQNRR896 pKa = 11.84 EE897 pKa = 3.72 LEE899 pKa = 4.62 GYY901 pKa = 10.75 DD902 pKa = 3.25 NFGATGAFWGHH913 pKa = 6.31 PPEE916 pKa = 6.01 HH917 pKa = 7.32 DD918 pKa = 4.04 FYY920 pKa = 11.46 DD921 pKa = 3.9 DD922 pKa = 3.6 TVIMDD927 pKa = 4.26 WAVDD931 pKa = 3.43 AVYY934 pKa = 10.73 AFQAEE939 pKa = 5.03 GYY941 pKa = 8.67 EE942 pKa = 4.05 YY943 pKa = 9.77 TARR946 pKa = 11.84 GEE948 pKa = 4.11 FDD950 pKa = 2.68 WGYY953 pKa = 11.45 GWFTEE958 pKa = 4.56 YY959 pKa = 7.85 TTNPQPHH966 pKa = 5.68 YY967 pKa = 11.23 VRR969 pKa = 11.84 TLDD972 pKa = 3.67 DD973 pKa = 3.98 RR974 pKa = 11.84 NVRR977 pKa = 11.84 MTFMGYY983 pKa = 10.5 LSHH986 pKa = 7.94 DD987 pKa = 3.87 GYY989 pKa = 11.54 NNNWLSNHH997 pKa = 5.71 SPAFVPFMKK1006 pKa = 10.46 SQVDD1010 pKa = 3.78 QILKK1014 pKa = 10.51 ANPDD1018 pKa = 3.23 KK1019 pKa = 11.52 LMFDD1023 pKa = 3.57 TQTNSTRR1030 pKa = 11.84 STDD1033 pKa = 3.25 MRR1035 pKa = 11.84 DD1036 pKa = 3.24 FGGDD1040 pKa = 3.54 FSPYY1044 pKa = 9.96 AMEE1047 pKa = 4.92 NFRR1050 pKa = 11.84 VWLSKK1055 pKa = 10.49 KK1056 pKa = 10.39 YY1057 pKa = 9.44 STGEE1061 pKa = 3.73 LAALGINDD1069 pKa = 4.36 INSFDD1074 pKa = 3.68 YY1075 pKa = 11.46 GDD1077 pKa = 3.75 FLRR1080 pKa = 11.84 AQGVTHH1086 pKa = 6.5 TSWSNAGDD1094 pKa = 3.76 TLSGNIPLQEE1104 pKa = 4.06 DD1105 pKa = 4.21 YY1106 pKa = 10.91 IYY1108 pKa = 10.85 FNRR1111 pKa = 11.84 DD1112 pKa = 1.96 VWNQKK1117 pKa = 8.47 FAEE1120 pKa = 4.14 VLDD1123 pKa = 4.67 YY1124 pKa = 10.77 IRR1126 pKa = 11.84 QQQPDD1131 pKa = 3.68 IEE1133 pKa = 5.14 IGASTHH1139 pKa = 5.75 LFEE1142 pKa = 5.23 SRR1144 pKa = 11.84 GYY1146 pKa = 10.33 VFNEE1150 pKa = 3.35 NLTFLSGEE1158 pKa = 4.24 LNLGARR1164 pKa = 11.84 TTISEE1169 pKa = 4.63 LPTNILVHH1177 pKa = 6.42 LKK1179 pKa = 10.2 GAQAVDD1185 pKa = 3.12 KK1186 pKa = 9.61 TLVYY1190 pKa = 10.12 FPYY1193 pKa = 9.78 PWEE1196 pKa = 4.08 FDD1198 pKa = 3.43 EE1199 pKa = 6.74 LRR1201 pKa = 11.84 LQDD1204 pKa = 3.35 APRR1207 pKa = 11.84 FGRR1210 pKa = 11.84 GWVAQAYY1217 pKa = 9.84 AYY1219 pKa = 10.24 GGLFSIPANVWVGGEE1234 pKa = 3.93 VWTWSPGADD1243 pKa = 3.33 NYY1245 pKa = 11.19 RR1246 pKa = 11.84 DD1247 pKa = 2.94 IYY1249 pKa = 11.15 LFVRR1253 pKa = 11.84 AQADD1257 pKa = 3.7 LLDD1260 pKa = 5.57 DD1261 pKa = 3.66 YY1262 pKa = 11.46 TSYY1265 pKa = 11.69 SKK1267 pKa = 11.15 VGLVHH1272 pKa = 7.3 AMYY1275 pKa = 11.07 SSMKK1279 pKa = 10.49 AGFIDD1284 pKa = 4.46 GGNQIQSSTKK1294 pKa = 10.39 LLTEE1298 pKa = 4.09 GNINFDD1304 pKa = 3.9 LLVFGDD1310 pKa = 3.51 EE1311 pKa = 4.94 GYY1313 pKa = 9.94 PVVPRR1318 pKa = 11.84 PEE1320 pKa = 5.09 DD1321 pKa = 3.49 FDD1323 pKa = 4.58 KK1324 pKa = 10.84 FDD1326 pKa = 5.44 HH1327 pKa = 6.72 IFFDD1331 pKa = 4.82 GDD1333 pKa = 3.54 QQYY1336 pKa = 7.85 LTAEE1340 pKa = 4.11 QQALLDD1346 pKa = 3.79 QQGDD1350 pKa = 3.81 KK1351 pKa = 10.67 VRR1353 pKa = 11.84 HH1354 pKa = 5.17 IGQRR1358 pKa = 11.84 GTVSGIEE1365 pKa = 3.65 ITVSINGTEE1374 pKa = 4.17 SNEE1377 pKa = 4.15 TVSAVSRR1384 pKa = 11.84 IHH1386 pKa = 5.76 EE1387 pKa = 4.15 TDD1389 pKa = 2.75 AAAPYY1394 pKa = 9.81 VVHH1397 pKa = 6.67 LVNRR1401 pKa = 11.84 PFAGGVTPTLNNVEE1415 pKa = 4.2 VAIPQSYY1422 pKa = 9.36 FPEE1425 pKa = 4.52 VVTGATLHH1433 pKa = 6.63 LPDD1436 pKa = 4.49 GTSTPLTLSTNADD1449 pKa = 3.39 GDD1451 pKa = 4.0 VVLPVNNLEE1460 pKa = 3.82 VWGILEE1466 pKa = 4.3 LAHH1469 pKa = 7.01
Molecular weight: 159.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.808
IPC_protein 3.859
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.681
Grimsley 3.528
Solomon 3.846
Lehninger 3.808
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.795
Sillero 3.973
Patrickios 1.227
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A1I0GDB0|A0A1I0GDB0_THASX Xaa-Pro dipeptidase OS=Thalassotalea agarivorans OX=349064 GN=pepQ PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.39 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2994
0
2994
993003
27
3292
331.7
36.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.893 ± 0.044
0.925 ± 0.014
5.871 ± 0.042
6.102 ± 0.038
4.408 ± 0.03
6.679 ± 0.046
2.18 ± 0.024
6.527 ± 0.04
5.674 ± 0.041
9.815 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.438 ± 0.022
4.529 ± 0.031
3.798 ± 0.024
4.661 ± 0.041
3.992 ± 0.031
6.434 ± 0.034
5.482 ± 0.034
7.091 ± 0.04
1.217 ± 0.015
3.282 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here