Beihai weivirus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KL70|A0A1L3KL70_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 1 OX=1922738 PE=4 SV=1
MM1 pKa = 7.6LSALAPSPLWAHH13 pKa = 6.5HH14 pKa = 6.32LHH16 pKa = 6.99PDD18 pKa = 3.74CLAAAYY24 pKa = 9.3EE25 pKa = 4.37RR26 pKa = 11.84DD27 pKa = 3.84GAWEE31 pKa = 4.26VEE33 pKa = 4.14THH35 pKa = 5.51EE36 pKa = 4.19PRR38 pKa = 11.84VRR40 pKa = 11.84TLLGAFRR47 pKa = 11.84ARR49 pKa = 11.84LFGSSGVTTTRR60 pKa = 11.84ITLTEE65 pKa = 4.02KK66 pKa = 10.7EE67 pKa = 4.36FAKK70 pKa = 10.94LMDD73 pKa = 3.51VAKK76 pKa = 9.73QHH78 pKa = 6.16LMAEE82 pKa = 4.37AKK84 pKa = 10.36EE85 pKa = 4.24KK86 pKa = 10.68VLRR89 pKa = 11.84AAVNMEE95 pKa = 4.16ANGLWRR101 pKa = 11.84TGSVEE106 pKa = 4.16HH107 pKa = 6.66VGLKK111 pKa = 10.37ALAPDD116 pKa = 3.48MVIRR120 pKa = 11.84CAVGAQVTDD129 pKa = 3.7ATSVAPLLSAPLLFAAAAYY148 pKa = 7.12CQPAIARR155 pKa = 11.84FAVEE159 pKa = 4.02GLRR162 pKa = 11.84WLAKK166 pKa = 9.91RR167 pKa = 11.84CFEE170 pKa = 4.1VPFWEE175 pKa = 4.47KK176 pKa = 8.63EE177 pKa = 4.16TRR179 pKa = 11.84SLVMLLEE186 pKa = 4.33GVYY189 pKa = 10.48APEE192 pKa = 3.99TRR194 pKa = 11.84VVQGWPRR201 pKa = 11.84PRR203 pKa = 11.84APVIMAVCGFGWVGVCLWRR222 pKa = 11.84SYY224 pKa = 11.77NEE226 pKa = 3.89VAQIWTSTYY235 pKa = 10.0RR236 pKa = 11.84PSTRR240 pKa = 11.84RR241 pKa = 11.84RR242 pKa = 11.84RR243 pKa = 11.84AGGGAEE249 pKa = 4.33APPQGGEE256 pKa = 3.77VGVRR260 pKa = 11.84RR261 pKa = 11.84EE262 pKa = 4.06PGGAEE267 pKa = 4.09ARR269 pKa = 11.84DD270 pKa = 4.01EE271 pKa = 4.34PAPLPPGPPPPPAPPPAPAILAPPALAILAPPPALAILDD310 pKa = 4.54RR311 pKa = 11.84EE312 pKa = 4.54PEE314 pKa = 4.13DD315 pKa = 3.94APAPEE320 pKa = 4.05VEE322 pKa = 4.34MRR324 pKa = 11.84EE325 pKa = 4.19QEE327 pKa = 4.25QSTVAARR334 pKa = 11.84VEE336 pKa = 4.07EE337 pKa = 4.52SEE339 pKa = 5.23DD340 pKa = 3.56LVLQEE345 pKa = 3.21PHH347 pKa = 5.04GRR349 pKa = 11.84YY350 pKa = 7.91RR351 pKa = 11.84TVEE354 pKa = 3.77VGGEE358 pKa = 3.99RR359 pKa = 11.84VHH361 pKa = 7.06VVLGQDD367 pKa = 3.41YY368 pKa = 11.13DD369 pKa = 3.69KK370 pKa = 11.26DD371 pKa = 3.86APRR374 pKa = 11.84DD375 pKa = 3.34RR376 pKa = 11.84APRR379 pKa = 11.84VGALTGPSQKK389 pKa = 10.2PPNVYY394 pKa = 10.9SNTKK398 pKa = 9.88KK399 pKa = 10.82NIIAAIYY406 pKa = 9.58HH407 pKa = 5.69RR408 pKa = 11.84LEE410 pKa = 4.82LKK412 pKa = 10.56SKK414 pKa = 9.04PCKK417 pKa = 8.97FTAADD422 pKa = 3.28KK423 pKa = 10.93RR424 pKa = 11.84KK425 pKa = 9.47IGKK428 pKa = 8.79FVAAAISNKK437 pKa = 10.35GIFSRR442 pKa = 11.84KK443 pKa = 8.77RR444 pKa = 11.84VEE446 pKa = 3.32AWFRR450 pKa = 11.84KK451 pKa = 9.97HH452 pKa = 6.84FDD454 pKa = 2.91ITEE457 pKa = 3.93WRR459 pKa = 11.84SKK461 pKa = 9.98KK462 pKa = 9.29WSLEE466 pKa = 3.9RR467 pKa = 11.84IVQMIEE473 pKa = 3.86RR474 pKa = 11.84LLCQVDD480 pKa = 3.34PEE482 pKa = 4.49FRR484 pKa = 11.84LNTAVKK490 pKa = 10.5AEE492 pKa = 4.32DD493 pKa = 3.65MPEE496 pKa = 4.15GKK498 pKa = 9.76SPRR501 pKa = 11.84FLIADD506 pKa = 4.08GDD508 pKa = 4.15AGQVMALAAIKK519 pKa = 10.44CIEE522 pKa = 4.46DD523 pKa = 3.6LLFEE527 pKa = 4.49AFEE530 pKa = 4.08EE531 pKa = 4.53HH532 pKa = 6.93SIKK535 pKa = 10.68HH536 pKa = 5.27AGKK539 pKa = 9.06RR540 pKa = 11.84DD541 pKa = 3.3AVARR545 pKa = 11.84LVGHH549 pKa = 5.86MRR551 pKa = 11.84VPSKK555 pKa = 10.42RR556 pKa = 11.84RR557 pKa = 11.84RR558 pKa = 11.84HH559 pKa = 5.29GFCFVEE565 pKa = 4.65GDD567 pKa = 3.36GSAWDD572 pKa = 3.95TTCNEE577 pKa = 4.25TVRR580 pKa = 11.84GLIEE584 pKa = 4.22NPVIAHH590 pKa = 6.04VGAIIAEE597 pKa = 4.28MGLVPASWVKK607 pKa = 10.62AHH609 pKa = 6.54EE610 pKa = 4.65KK611 pKa = 10.33VNQSKK616 pKa = 10.51KK617 pKa = 10.25YY618 pKa = 10.16KK619 pKa = 10.3LFFKK623 pKa = 10.46KK624 pKa = 9.94FHH626 pKa = 5.83EE627 pKa = 4.47VLRR630 pKa = 11.84KK631 pKa = 9.32EE632 pKa = 3.76IPAIRR637 pKa = 11.84RR638 pKa = 11.84SGHH641 pKa = 6.31RR642 pKa = 11.84GTSVLNYY649 pKa = 8.32WVNFAMWVCSLFEE662 pKa = 4.33APEE665 pKa = 4.18KK666 pKa = 10.79FLDD669 pKa = 3.56PEE671 pKa = 4.16VRR673 pKa = 11.84SGVDD677 pKa = 3.11VAGMDD682 pKa = 2.82RR683 pKa = 11.84WFFGGFEE690 pKa = 4.38GDD692 pKa = 3.51DD693 pKa = 4.01SGVQTAPRR701 pKa = 11.84LIAVSDD707 pKa = 3.74EE708 pKa = 3.65DD709 pKa = 4.22RR710 pKa = 11.84AALRR714 pKa = 11.84AGQKK718 pKa = 9.14QASDD722 pKa = 4.08LAQGDD727 pKa = 3.95PFITEE732 pKa = 4.33SVVKK736 pKa = 10.56ASAEE740 pKa = 3.88ALDD743 pKa = 3.73FWDD746 pKa = 4.25RR747 pKa = 11.84GGFNMKK753 pKa = 9.98FVFASRR759 pKa = 11.84RR760 pKa = 11.84ATMVGMHH767 pKa = 6.55LHH769 pKa = 6.97LSNDD773 pKa = 3.51NGNTEE778 pKa = 4.13PSGLFCPEE786 pKa = 4.25LPRR789 pKa = 11.84GIGKK793 pKa = 8.79NVSCSPAILAAIEE806 pKa = 4.32SGCLRR811 pKa = 11.84EE812 pKa = 4.21VKK814 pKa = 10.36KK815 pKa = 10.65IAAAANLARR824 pKa = 11.84AADD827 pKa = 3.87YY828 pKa = 11.24AGILPTVSNKK838 pKa = 9.73YY839 pKa = 10.01KK840 pKa = 10.46EE841 pKa = 4.15YY842 pKa = 10.89ADD844 pKa = 4.41RR845 pKa = 11.84LDD847 pKa = 3.61AGNYY851 pKa = 6.99QDD853 pKa = 5.83RR854 pKa = 11.84EE855 pKa = 4.15MAMHH859 pKa = 6.75VDD861 pKa = 3.53GEE863 pKa = 4.46EE864 pKa = 4.15GVTAVDD870 pKa = 3.24VRR872 pKa = 11.84EE873 pKa = 4.29RR874 pKa = 11.84IEE876 pKa = 4.13LLNSSVTPADD886 pKa = 3.58EE887 pKa = 4.2EE888 pKa = 4.32DD889 pKa = 3.82RR890 pKa = 11.84LAKK893 pKa = 10.37LLYY896 pKa = 10.59SADD899 pKa = 3.79EE900 pKa = 4.81DD901 pKa = 3.65EE902 pKa = 4.45MQRR905 pKa = 11.84FKK907 pKa = 11.35DD908 pKa = 3.97YY909 pKa = 11.28LWDD912 pKa = 4.45FSNLDD917 pKa = 3.38DD918 pKa = 4.27HH919 pKa = 7.18AGYY922 pKa = 9.47HH923 pKa = 6.4ASLPAAWRR931 pKa = 11.84AGGSVV936 pKa = 2.91

Molecular weight: 103.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KL70|A0A1L3KL70_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 1 OX=1922738 PE=4 SV=1
MM1 pKa = 6.68TRR3 pKa = 11.84SRR5 pKa = 11.84AARR8 pKa = 11.84RR9 pKa = 11.84PQRR12 pKa = 11.84GNAAQAAPKK21 pKa = 9.43RR22 pKa = 11.84QPAPRR27 pKa = 11.84PAPRR31 pKa = 11.84RR32 pKa = 11.84AGARR36 pKa = 11.84RR37 pKa = 11.84QRR39 pKa = 11.84RR40 pKa = 11.84GGNARR45 pKa = 11.84GRR47 pKa = 11.84GVDD50 pKa = 4.4LFDD53 pKa = 4.81PRR55 pKa = 11.84NTMLVPSVVSEE66 pKa = 3.91GMAFPMGGSAVRR78 pKa = 11.84PFNAGTTYY86 pKa = 10.82RR87 pKa = 11.84SLIAVTNTGSSGSILSWLQNAATPSTGVDD116 pKa = 4.05TIPLLSLADD125 pKa = 3.9DD126 pKa = 4.59AGGPTAGRR134 pKa = 11.84AMKK137 pKa = 10.56AGITIVNRR145 pKa = 11.84TQNLNKK151 pKa = 10.02GGQVTVLNAVQRR163 pKa = 11.84VLLPAAPSALTQAQWDD179 pKa = 4.08TFLSKK184 pKa = 10.69VVSHH188 pKa = 7.15PSSTIYY194 pKa = 10.9DD195 pKa = 3.62GTDD198 pKa = 3.3FAKK201 pKa = 10.35PKK203 pKa = 10.26TFICHH208 pKa = 6.9PLDD211 pKa = 3.17QTDD214 pKa = 3.55YY215 pKa = 10.51LKK217 pKa = 11.23YY218 pKa = 10.46GGWHH222 pKa = 5.68GTDD225 pKa = 3.16TLDD228 pKa = 4.68EE229 pKa = 4.64FWSHH233 pKa = 4.9IAIWPGHH240 pKa = 5.85NPDD243 pKa = 4.24SRR245 pKa = 11.84PMSTIFVVIEE255 pKa = 3.84QPAVQNAYY263 pKa = 8.02EE264 pKa = 4.12LKK266 pKa = 10.68ARR268 pKa = 11.84ARR270 pKa = 11.84YY271 pKa = 5.43YY272 pKa = 10.02TRR274 pKa = 11.84WPLDD278 pKa = 3.43SVGGQAQGVVPTASANVVNAARR300 pKa = 11.84DD301 pKa = 3.49RR302 pKa = 11.84AEE304 pKa = 4.05ATAHH308 pKa = 6.3IPRR311 pKa = 11.84EE312 pKa = 4.2GAVAAAGAAVMGGLGWVARR331 pKa = 11.84GARR334 pKa = 11.84AGAAAIEE341 pKa = 4.39GAGALMEE348 pKa = 5.05PLLPMAAMLL357 pKa = 4.61

Molecular weight: 37.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1293

357

936

646.5

70.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.766 ± 1.034

1.237 ± 0.45

4.95 ± 0.352

6.342 ± 1.927

3.403 ± 0.546

7.966 ± 0.995

2.243 ± 0.264

3.712 ± 0.033

4.331 ± 0.982

7.579 ± 0.403

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.19

2.862 ± 0.498

6.961 ± 0.283

2.784 ± 0.666

7.966 ± 0.206

5.336 ± 0.257

4.486 ± 1.183

7.657 ± 0.307

2.088 ± 0.06

1.933 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski