Podila verticillata NRRL 6337

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mortierellomycotina; Mortierellomycetes; Mortierellales; Mortierellaceae; Podila; Podila verticillata

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086TM33|A0A086TM33_9FUNG Uncharacterized protein OS=Podila verticillata NRRL 6337 OX=1069443 GN=MVEG_11048 PE=4 SV=1
MM1 pKa = 6.78YY2 pKa = 10.18VAVVGVGSPATNYY15 pKa = 10.96DD16 pKa = 4.21LIVDD20 pKa = 4.43TGSSNTWVGASKK32 pKa = 10.76SYY34 pKa = 10.67TPTSTSQQTSDD45 pKa = 3.3SVSVTEE51 pKa = 4.36SGSTGFSGTEE61 pKa = 3.64YY62 pKa = 10.27TDD64 pKa = 3.33QVTLASGLVVSGQSIGVASTAKK86 pKa = 10.29GFKK89 pKa = 10.41DD90 pKa = 3.16VDD92 pKa = 4.48GILGLGPVDD101 pKa = 3.76LTVGTLSPDD110 pKa = 3.17TSSAIPTVVDD120 pKa = 3.35NLFSQGSIPGHH131 pKa = 5.4EE132 pKa = 4.06CSVYY136 pKa = 10.44FEE138 pKa = 5.97PITDD142 pKa = 3.39SSGIQQNGQITFGGTDD158 pKa = 3.03SSKK161 pKa = 9.12FTGEE165 pKa = 3.2ITYY168 pKa = 10.87APITTTSPASNYY180 pKa = 9.06WGVDD184 pKa = 2.82ASFSYY189 pKa = 11.16GSGSDD194 pKa = 3.11STTILSATAGIVDD207 pKa = 4.18SGTTLLPLATDD218 pKa = 4.54AFNSFQKK225 pKa = 10.15ATGGVKK231 pKa = 10.38DD232 pKa = 3.84SATGLLSITSQQFGNMKK249 pKa = 9.96SLYY252 pKa = 8.68VTISDD257 pKa = 3.83TVFEE261 pKa = 4.62LTPNALIWPRR271 pKa = 11.84SLNSIIGGTAGSIYY285 pKa = 10.53LIVTDD290 pKa = 4.17NGSNSGSGLDD300 pKa = 4.44FILGQKK306 pKa = 10.09FMEE309 pKa = 4.35RR310 pKa = 11.84FYY312 pKa = 11.08TVFDD316 pKa = 3.59TANQRR321 pKa = 11.84VGFATTPFTDD331 pKa = 3.16ATSNN335 pKa = 3.34

Molecular weight: 34.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086TKU8|A0A086TKU8_9FUNG 4-aminobutyrate aminotransferase OS=Podila verticillata NRRL 6337 OX=1069443 GN=MVEG_11968 PE=3 SV=1
MM1 pKa = 7.79PLFKK5 pKa = 10.46RR6 pKa = 11.84RR7 pKa = 11.84APVQHH12 pKa = 5.28VHH14 pKa = 6.05HH15 pKa = 7.12RR16 pKa = 11.84PSTMARR22 pKa = 11.84LKK24 pKa = 11.41AMFTPYY30 pKa = 9.95HH31 pKa = 6.16HH32 pKa = 7.87PYY34 pKa = 9.61NHH36 pKa = 6.66AHH38 pKa = 6.05GHH40 pKa = 4.81SQAPAPVSTQARR52 pKa = 11.84AAKK55 pKa = 9.72RR56 pKa = 11.84SLFGRR61 pKa = 11.84TPGAHH66 pKa = 6.7HH67 pKa = 7.27PTTATHH73 pKa = 5.21TRR75 pKa = 11.84RR76 pKa = 11.84RR77 pKa = 11.84NPFARR82 pKa = 11.84RR83 pKa = 11.84MGGTAQAAAVATAGHH98 pKa = 5.9HH99 pKa = 6.29HH100 pKa = 6.38GHH102 pKa = 6.87HH103 pKa = 6.23GHH105 pKa = 6.75HH106 pKa = 6.27GHH108 pKa = 7.13RR109 pKa = 11.84SPLASLKK116 pKa = 11.16AMFRR120 pKa = 11.84PRR122 pKa = 11.84RR123 pKa = 11.84SHH125 pKa = 4.91GHH127 pKa = 4.25SHH129 pKa = 6.8RR130 pKa = 11.84SHH132 pKa = 5.56YY133 pKa = 9.87TSS135 pKa = 3.45

Molecular weight: 15.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1503

11

1514

708146

47

5427

467.7

51.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.766 ± 0.068

1.238 ± 0.029

5.542 ± 0.048

5.903 ± 0.062

3.571 ± 0.037

6.142 ± 0.059

2.854 ± 0.04

5.075 ± 0.049

5.153 ± 0.068

9.392 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.026

4.293 ± 0.055

5.508 ± 0.077

4.514 ± 0.066

5.225 ± 0.054

9.018 ± 0.095

6.471 ± 0.058

5.945 ± 0.053

1.201 ± 0.022

2.802 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski