Streptococcus satellite phage Javan624

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTD2|A0A4D5ZTD2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan624 OX=2558791 GN=JavanS624_0014 PE=4 SV=1
MM1 pKa = 6.41EE2 pKa = 4.35QKK4 pKa = 10.77VIASYY9 pKa = 10.96SILCVEE15 pKa = 4.75LEE17 pKa = 3.97EE18 pKa = 4.4LLDD21 pKa = 4.21VIEE24 pKa = 5.47LGLDD28 pKa = 3.37DD29 pKa = 4.13GTPNKK34 pKa = 9.33MAATLNVVEE43 pKa = 4.8MALKK47 pKa = 10.77GIISVHH53 pKa = 4.62QEE55 pKa = 3.73NIKK58 pKa = 10.62VMEE61 pKa = 5.31KK62 pKa = 9.25IDD64 pKa = 4.04KK65 pKa = 10.45EE66 pKa = 4.11II67 pKa = 4.67

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZSH3|A0A4D5ZSH3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan624 OX=2558791 GN=JavanS624_0011 PE=4 SV=1
MM1 pKa = 7.48NIKK4 pKa = 9.69EE5 pKa = 4.18IKK7 pKa = 9.36KK8 pKa = 9.39KK9 pKa = 10.5NGSIVYY15 pKa = 9.54RR16 pKa = 11.84ANVYY20 pKa = 10.66LGTDD24 pKa = 2.86QMTGKK29 pKa = 10.28RR30 pKa = 11.84VRR32 pKa = 11.84TNVTAPTKK40 pKa = 10.49KK41 pKa = 10.03GVKK44 pKa = 9.34IKK46 pKa = 10.9ARR48 pKa = 11.84DD49 pKa = 3.74AINNFIANGNTVKK62 pKa = 10.68QKK64 pKa = 10.48VQIKK68 pKa = 9.11TYY70 pKa = 10.36EE71 pKa = 4.11EE72 pKa = 4.05LVKK75 pKa = 10.42IWWEE79 pKa = 4.16SYY81 pKa = 11.0KK82 pKa = 10.15NTVKK86 pKa = 10.78PNTRR90 pKa = 11.84QSMKK94 pKa = 10.83GIVKK98 pKa = 9.94LHH100 pKa = 6.11LLSVFGDD107 pKa = 3.58YY108 pKa = 10.87KK109 pKa = 10.99LNRR112 pKa = 11.84INTPLIQTQVNKK124 pKa = 9.13WANLANKK131 pKa = 8.81GVKK134 pKa = 9.53GAFANYY140 pKa = 9.86HH141 pKa = 6.24LLHH144 pKa = 6.49NLNSRR149 pKa = 11.84ILQYY153 pKa = 10.84GVAMQVIPSNPARR166 pKa = 11.84DD167 pKa = 3.48VLVPRR172 pKa = 11.84KK173 pKa = 9.06IAKK176 pKa = 9.72EE177 pKa = 3.91KK178 pKa = 11.06EE179 pKa = 3.69KK180 pKa = 11.01LKK182 pKa = 10.85YY183 pKa = 10.63LNNDD187 pKa = 3.08QLKK190 pKa = 8.34TFLAYY195 pKa = 10.64LDD197 pKa = 4.15TLDD200 pKa = 3.58QSEE203 pKa = 4.48YY204 pKa = 11.1QNLFDD209 pKa = 3.5VTLYY213 pKa = 8.93KK214 pKa = 10.34TLLATGCRR222 pKa = 11.84ISEE225 pKa = 4.17VLALEE230 pKa = 4.05WSDD233 pKa = 3.35IDD235 pKa = 5.1FEE237 pKa = 4.37NSQIKK242 pKa = 9.78INKK245 pKa = 6.81TLNRR249 pKa = 11.84FQEE252 pKa = 4.43TNTPKK257 pKa = 10.53SKK259 pKa = 10.86SSIRR263 pKa = 11.84NIDD266 pKa = 3.15IDD268 pKa = 3.91RR269 pKa = 11.84ATLLMLKK276 pKa = 9.74QYY278 pKa = 11.17KK279 pKa = 9.64NRR281 pKa = 11.84QQIEE285 pKa = 3.67AWQLGKK291 pKa = 9.04TEE293 pKa = 4.17TVVFSVFVTKK303 pKa = 10.52YY304 pKa = 10.63AYY306 pKa = 10.32ACNLRR311 pKa = 11.84KK312 pKa = 9.69RR313 pKa = 11.84LNKK316 pKa = 9.74HH317 pKa = 5.48FNNAGVTNVSFHH329 pKa = 6.04GFRR332 pKa = 11.84HH333 pKa = 4.95THH335 pKa = 5.72ASMMMNAGLPYY346 pKa = 10.22KK347 pKa = 9.91EE348 pKa = 4.02LQHH351 pKa = 6.76RR352 pKa = 11.84LGHH355 pKa = 5.56STLAMTMDD363 pKa = 4.52TYY365 pKa = 11.84SHH367 pKa = 7.28LSNEE371 pKa = 4.25NAKK374 pKa = 10.46KK375 pKa = 10.32AVSLFEE381 pKa = 3.89TAINNLL387 pKa = 3.55

Molecular weight: 44.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2215

57

387

158.2

18.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.734 ± 0.55

0.271 ± 0.093

5.327 ± 0.428

7.675 ± 0.612

4.063 ± 0.554

3.883 ± 0.33

1.4 ± 0.265

7.765 ± 0.53

10.79 ± 0.608

10.609 ± 0.55

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.28

7.178 ± 0.521

2.302 ± 0.337

4.966 ± 0.618

3.928 ± 0.454

6.185 ± 0.582

4.786 ± 0.576

6.14 ± 0.453

0.948 ± 0.151

3.476 ± 0.408
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski