Proteobacteria bacterium CAG:495

Taxonomy: cellular organisms; Bacteria; Proteobacteria; environmental samples

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1693 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5QQD0|R5QQD0_9PROT 50S ribosomal protein L10 OS=Proteobacteria bacterium CAG:495 OX=1262987 GN=rplJ PE=3 SV=1
MM1 pKa = 7.68NEE3 pKa = 3.88GFRR6 pKa = 11.84NLALLVWALCAVNSNSYY23 pKa = 9.97AQDD26 pKa = 3.17SATVITEE33 pKa = 4.11EE34 pKa = 4.67SSTSAPTIVYY44 pKa = 9.32GAARR48 pKa = 11.84RR49 pKa = 11.84ADD51 pKa = 3.52GEE53 pKa = 4.17TDD55 pKa = 3.16EE56 pKa = 5.42VLIEE60 pKa = 4.12QPAGASNPLGNPLPDD75 pKa = 4.09AAEE78 pKa = 3.99QQQPQAVTEE87 pKa = 4.15PVSGGNADD95 pKa = 3.87NAPAAAEE102 pKa = 3.94PMQDD106 pKa = 3.2VPNVQGQPVVSAPKK120 pKa = 10.29LGQEE124 pKa = 4.29FQNTLMEE131 pKa = 5.21ADD133 pKa = 3.34GMVYY137 pKa = 10.25DD138 pKa = 4.0VQAYY142 pKa = 6.21PTEE145 pKa = 4.15DD146 pKa = 3.03LKK148 pKa = 11.56AIGNSSNPEE157 pKa = 3.97TIYY160 pKa = 11.05SPNVNPP166 pKa = 5.06

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5QQU9|R5QQU9_9PROT DNA helicase OS=Proteobacteria bacterium CAG:495 OX=1262987 GN=BN682_01004 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.01VLAARR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.9KK41 pKa = 10.62LSAA44 pKa = 4.03

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1693

0

1693

496630

29

1840

293.3

32.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.409 ± 0.072

1.326 ± 0.047

5.578 ± 0.051

6.841 ± 0.07

4.407 ± 0.04

6.572 ± 0.063

1.592 ± 0.025

6.793 ± 0.059

7.101 ± 0.063

9.442 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.028

5.029 ± 0.047

3.768 ± 0.04

3.426 ± 0.038

4.854 ± 0.051

6.05 ± 0.047

4.79 ± 0.05

6.796 ± 0.056

1.009 ± 0.021

3.601 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski