Paenibacillus pectinilyticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6496 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C1A575|A0A1C1A575_9BACL ABC transmembrane type-1 domain-containing protein OS=Paenibacillus pectinilyticus OX=512399 GN=A8709_13105 PE=3 SV=1
MM1 pKa = 7.54TGPTGATGVTGDD13 pKa = 4.04TGPTGTTGVTGPTGATGVTGATGVTGVTGPTGATGVTGPTGATGVTGDD61 pKa = 4.2TGPTGATGVTGPTGATGVTGVTGVTGVTGPTGATGVTGDD100 pKa = 4.2TGPTGATGVTGPTGATGVTGATGVTGVTGPTGPTGATGVTGDD142 pKa = 4.2TGPTGATGVTGPTGATGVTGATGVTGVTGPTGATGVTGDD181 pKa = 4.2TGPTGATGVTGVTGPTGATGVTGATGATGVTGATGVTGVTGVTGVTGATGPTGPTGATGPSVLTEE246 pKa = 4.0SFSAFNSNLSTAATTQIANWSVANPYY272 pKa = 10.61YY273 pKa = 10.72NATTFNPTTGNFTVPATGTYY293 pKa = 9.27QFEE296 pKa = 4.11ATLNYY301 pKa = 8.83STTATLSISLGAGVNPAFVIRR322 pKa = 11.84RR323 pKa = 11.84TSPTVTDD330 pKa = 5.81LITGLFPILDD340 pKa = 3.72VNVALVLNLRR350 pKa = 11.84AVLGDD355 pKa = 3.53GVVTLTGALDD365 pKa = 3.74LTAGDD370 pKa = 4.08VVGLFYY376 pKa = 10.93VANGLTVSLNLGGNTSAGMYY396 pKa = 8.59WSVFRR401 pKa = 11.84IVV403 pKa = 3.07

Molecular weight: 37.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C1A7C6|A0A1C1A7C6_9BACL Spermidine/putrescine ABC transporter ATP-binding protein OS=Paenibacillus pectinilyticus OX=512399 GN=A8709_01555 PE=4 SV=1
MM1 pKa = 7.61GPTFNPNTRR10 pKa = 11.84KK11 pKa = 9.86RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 9.13NHH16 pKa = 5.06GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTKK25 pKa = 10.0NGRR28 pKa = 11.84KK29 pKa = 8.8VLQARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.11GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6496

0

6496

2286042

26

3161

351.9

39.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.977 ± 0.034

0.719 ± 0.009

5.176 ± 0.023

6.035 ± 0.035

4.25 ± 0.023

7.188 ± 0.032

2.167 ± 0.016

6.937 ± 0.03

5.451 ± 0.032

9.85 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.018

4.214 ± 0.031

3.953 ± 0.018

4.087 ± 0.023

4.373 ± 0.029

6.638 ± 0.028

6.065 ± 0.039

7.117 ± 0.029

1.348 ± 0.014

3.611 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski