Campylobacter lanienae
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1504 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X9SRH5|A0A1X9SRH5_9PROT Cell division protein FtsZ OS=Campylobacter lanienae OX=75658 GN=ftsZ PE=3 SV=1
MM1 pKa = 7.12 TNIVKK6 pKa = 10.55 FGAVVVAATAILSSSAAAQGVFVGVEE32 pKa = 3.96 GALLQSQVKK41 pKa = 10.38 LDD43 pKa = 4.73 DD44 pKa = 4.44 IYY46 pKa = 11.06 PSVLDD51 pKa = 4.31 DD52 pKa = 4.79 PKK54 pKa = 10.79 DD55 pKa = 3.55 TSIEE59 pKa = 4.01 LGLKK63 pKa = 9.93 GGYY66 pKa = 10.31 DD67 pKa = 3.88 FDD69 pKa = 4.9 AFRR72 pKa = 11.84 VWGGYY77 pKa = 7.09 SYY79 pKa = 9.71 RR80 pKa = 11.84 TKK82 pKa = 10.89 GSEE85 pKa = 4.12 DD86 pKa = 3.67 YY87 pKa = 11.44 NMKK90 pKa = 10.75 DD91 pKa = 2.78 GADD94 pKa = 4.5 FINGEE99 pKa = 4.64 FNWQTHH105 pKa = 6.28 NILVGADD112 pKa = 3.66 YY113 pKa = 10.24 TPSLTDD119 pKa = 3.2 SFKK122 pKa = 11.05 LALGAYY128 pKa = 7.31 TGLSIVKK135 pKa = 10.46 GEE137 pKa = 4.09 FSGQAEE143 pKa = 4.55 YY144 pKa = 8.32 TTDD147 pKa = 2.95 GLTYY151 pKa = 11.35 VMDD154 pKa = 4.52 NDD156 pKa = 3.87 STTGTGAIFGLKK168 pKa = 10.16 LGGIYY173 pKa = 10.13 EE174 pKa = 4.2 VVEE177 pKa = 3.98 NNEE180 pKa = 3.61 IEE182 pKa = 4.28 FGIKK186 pKa = 9.97 GDD188 pKa = 3.82 YY189 pKa = 7.79 QTTGIEE195 pKa = 4.3 DD196 pKa = 3.57 YY197 pKa = 11.58 DD198 pKa = 4.25 NILNYY203 pKa = 10.09 GVYY206 pKa = 10.08 VGYY209 pKa = 10.55 NYY211 pKa = 10.94 KK212 pKa = 10.51 FF213 pKa = 3.32
Molecular weight: 23.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.844
IPC2_protein 4.062
IPC_protein 4.037
Toseland 3.834
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.948
Rodwell 3.872
Grimsley 3.745
Solomon 4.024
Lehninger 3.973
Nozaki 4.139
DTASelect 4.368
Thurlkill 3.872
EMBOSS 3.961
Sillero 4.164
Patrickios 0.871
IPC_peptide 4.012
IPC2_peptide 4.139
IPC2.peptide.svr19 4.042
Protein with the highest isoelectric point:
>tr|A0A381D8V0|A0A381D8V0_9PROT Acetolactate synthase OS=Campylobacter lanienae OX=75658 GN=ilvH PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPHH8 pKa = 5.76 KK9 pKa = 7.92 TPKK12 pKa = 9.96 KK13 pKa = 7.47 RR14 pKa = 11.84 THH16 pKa = 5.94 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.07 TKK25 pKa = 10.15 NGRR28 pKa = 11.84 KK29 pKa = 9.33 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.31 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LAAA44 pKa = 4.44
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1504
0
1504
472411
14
1507
314.1
35.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.415 ± 0.061
1.119 ± 0.026
5.832 ± 0.052
6.123 ± 0.062
5.014 ± 0.059
6.221 ± 0.063
1.528 ± 0.021
9.513 ± 0.066
8.201 ± 0.059
9.706 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.388 ± 0.03
6.169 ± 0.066
2.95 ± 0.034
2.922 ± 0.036
3.472 ± 0.04
7.227 ± 0.054
4.316 ± 0.039
5.5 ± 0.051
0.675 ± 0.019
3.709 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here