Wenling narna-like virus 2
Average proteome isoelectric point is 5.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIM5|A0A1L3KIM5_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 2 OX=1923502 PE=4 SV=1
MM1 pKa = 7.58 SPRR4 pKa = 11.84 NLFPSYY10 pKa = 11.42 GDD12 pKa = 3.34 GCSSVNSTEE21 pKa = 3.85 NTLASILEE29 pKa = 4.65 SPFHH33 pKa = 6.14 PAWSAVVEE41 pKa = 4.36 GFFGNEE47 pKa = 3.95 VVTDD51 pKa = 3.47 THH53 pKa = 7.85 VDD55 pKa = 3.26 EE56 pKa = 4.57 LAIYY60 pKa = 10.4 EE61 pKa = 4.8 DD62 pKa = 4.39 GLDD65 pKa = 3.47 LSAYY69 pKa = 7.12 STSSVEE75 pKa = 4.01 QVVRR79 pKa = 11.84 CCEE82 pKa = 4.2 VYY84 pKa = 10.92 SFIYY88 pKa = 9.81 DD89 pKa = 3.18 QYY91 pKa = 10.67 GYY93 pKa = 11.09 KK94 pKa = 10.37 FLFSPTDD101 pKa = 3.15 QRR103 pKa = 11.84 LWLEE107 pKa = 4.67 LGDD110 pKa = 4.3 KK111 pKa = 10.78 DD112 pKa = 5.12 LLEE115 pKa = 5.36 VYY117 pKa = 9.85 LKK119 pKa = 10.39 WITANILAAVWEE131 pKa = 4.05 QDD133 pKa = 3.3 EE134 pKa = 4.99 LVPLPSSLLDD144 pKa = 3.94 FSARR148 pKa = 11.84 FPAFGLTTRR157 pKa = 11.84 DD158 pKa = 3.23 DD159 pKa = 3.68 RR160 pKa = 11.84 LFRR163 pKa = 11.84 SMIRR167 pKa = 11.84 SSRR170 pKa = 11.84 RR171 pKa = 11.84 SKK173 pKa = 9.1 KK174 pKa = 6.89 QQRR177 pKa = 11.84 FVYY180 pKa = 9.7 TLYY183 pKa = 10.45 QGKK186 pKa = 9.96 AGCNAMRR193 pKa = 11.84 PEE195 pKa = 4.09 LVAAKK200 pKa = 9.9 VVSAVEE206 pKa = 4.11 TLATPPEE213 pKa = 4.46 GEE215 pKa = 4.06 EE216 pKa = 4.15 SIGILGHH223 pKa = 7.43 LITKK227 pKa = 10.06 EE228 pKa = 4.06 DD229 pKa = 3.26 IRR231 pKa = 11.84 GQLLRR236 pKa = 11.84 TVTEE240 pKa = 4.35 IYY242 pKa = 9.18 GTGHH246 pKa = 5.11 ARR248 pKa = 11.84 EE249 pKa = 3.96 RR250 pKa = 11.84 RR251 pKa = 11.84 TPGSRR256 pKa = 11.84 RR257 pKa = 11.84 MASSKK262 pKa = 11.08 SSFQYY267 pKa = 9.93 TRR269 pKa = 11.84 SKK271 pKa = 11.11 GGAHH275 pKa = 6.73 EE276 pKa = 4.31 FLVDD280 pKa = 3.54 NAPASMFIQPRR291 pKa = 11.84 ILSGFVVYY299 pKa = 8.69 KK300 pKa = 8.71 TQVRR304 pKa = 11.84 PYY306 pKa = 8.49 YY307 pKa = 8.73 TTFQAEE313 pKa = 4.31 DD314 pKa = 3.39 WEE316 pKa = 4.39 EE317 pKa = 3.84 LQEE320 pKa = 4.11 LSRR323 pKa = 11.84 RR324 pKa = 11.84 AAWSEE329 pKa = 3.74 EE330 pKa = 3.74 MDD332 pKa = 4.54 CYY334 pKa = 10.86 PVGLPEE340 pKa = 4.1 AFKK343 pKa = 11.24 VRR345 pKa = 11.84 VITKK349 pKa = 10.1 GAVHH353 pKa = 7.27 HH354 pKa = 6.45 YY355 pKa = 9.81 NLARR359 pKa = 11.84 RR360 pKa = 11.84 WQPAMWEE367 pKa = 4.08 PLANHH372 pKa = 6.36 PTFEE376 pKa = 4.87 LVGTPNCQRR385 pKa = 11.84 IMNRR389 pKa = 11.84 FLAKK393 pKa = 10.09 CDD395 pKa = 4.01 PDD397 pKa = 3.93 DD398 pKa = 5.29 DD399 pKa = 4.47 RR400 pKa = 11.84 AFTSGDD406 pKa = 3.42 YY407 pKa = 11.15 ASATDD412 pKa = 4.39 YY413 pKa = 11.46 LDD415 pKa = 4.3 SDD417 pKa = 4.42 LSRR420 pKa = 11.84 SCLEE424 pKa = 4.05 YY425 pKa = 10.13 VCNALRR431 pKa = 11.84 VPFEE435 pKa = 4.14 DD436 pKa = 3.52 TVILAEE442 pKa = 4.33 ALTDD446 pKa = 3.63 HH447 pKa = 7.06 RR448 pKa = 11.84 LHH450 pKa = 6.47 YY451 pKa = 10.4 VDD453 pKa = 4.94 SEE455 pKa = 4.21 GVEE458 pKa = 3.9 QVKK461 pKa = 7.79 QQRR464 pKa = 11.84 RR465 pKa = 11.84 GQLMGSPISFPILCLFNAALTRR487 pKa = 11.84 YY488 pKa = 8.88 ALEE491 pKa = 4.16 IASCDD496 pKa = 3.65 SVALDD501 pKa = 5.36 LEE503 pKa = 4.59 DD504 pKa = 6.02 LPMLINGDD512 pKa = 4.11 DD513 pKa = 4.02 LLCRR517 pKa = 11.84 TNPLEE522 pKa = 3.87 YY523 pKa = 10.49 LVWKK527 pKa = 10.26 DD528 pKa = 3.07 IVNFGGLTPSIGKK541 pKa = 8.67 NFRR544 pKa = 11.84 HH545 pKa = 5.35 RR546 pKa = 11.84 TIGTINSEE554 pKa = 3.5 MWTFKK559 pKa = 9.85 HH560 pKa = 6.32 TIHH563 pKa = 6.83 GCPANGTDD571 pKa = 2.94 TAFYY575 pKa = 9.61 QGKK578 pKa = 8.26 RR579 pKa = 11.84 QPIIEE584 pKa = 4.39 IGLVRR589 pKa = 11.84 GSVKK593 pKa = 10.44 RR594 pKa = 11.84 GTINRR599 pKa = 11.84 NVDD602 pKa = 3.49 EE603 pKa = 5.25 LSPFVDD609 pKa = 3.08 SSKK612 pKa = 10.53 FGKK615 pKa = 10.41 SKK617 pKa = 8.53 EE618 pKa = 4.12 QCWNRR623 pKa = 11.84 FLDD626 pKa = 3.67 TCPNRR631 pKa = 11.84 LTAYY635 pKa = 10.0 DD636 pKa = 3.87 FLWRR640 pKa = 11.84 SVSSDD645 pKa = 2.73 ILEE648 pKa = 4.23 SLPRR652 pKa = 11.84 GMPMCCPTWLGGAGFPLPPLGHH674 pKa = 7.28 ALRR677 pKa = 11.84 DD678 pKa = 3.57 RR679 pKa = 11.84 RR680 pKa = 11.84 EE681 pKa = 3.58 PSAYY685 pKa = 9.47 QRR687 pKa = 11.84 LLARR691 pKa = 11.84 YY692 pKa = 9.27 LYY694 pKa = 8.96 DD695 pKa = 3.06 TYY697 pKa = 11.46 GYY699 pKa = 10.71 GLGKK703 pKa = 10.1 RR704 pKa = 11.84 YY705 pKa = 10.64 VSMLEE710 pKa = 4.19 TGSLPANLVKK720 pKa = 10.59 EE721 pKa = 4.32 MNTDD725 pKa = 2.9 NGIRR729 pKa = 11.84 DD730 pKa = 3.68 VLGVPKK736 pKa = 9.91 PVPEE740 pKa = 4.51 SADD743 pKa = 3.38 SVDD746 pKa = 4.21 GIKK749 pKa = 11.04 GNLHH753 pKa = 5.6 SEE755 pKa = 4.27 KK756 pKa = 10.24 TPLTPLPQTHH766 pKa = 6.83 FFMCGLINHH775 pKa = 6.21 TEE777 pKa = 4.04 EE778 pKa = 4.22 EE779 pKa = 4.43 STGRR783 pKa = 11.84 FSCEE787 pKa = 3.08 KK788 pKa = 9.17 MYY790 pKa = 11.24 SRR792 pKa = 11.84 LQHH795 pKa = 6.56 KK796 pKa = 9.41 ALKK799 pKa = 9.78 HH800 pKa = 4.86 GRR802 pKa = 11.84 GPLDD806 pKa = 3.56 CKK808 pKa = 10.67 FFLPTPPSLKK818 pKa = 10.17 LEE820 pKa = 4.13 SYY822 pKa = 10.19 VFQEE826 pKa = 4.43 VVAA829 pKa = 4.65
Molecular weight: 93.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.67
IPC2_protein 5.703
IPC_protein 5.741
Toseland 6.046
ProMoST 6.084
Dawson 5.982
Bjellqvist 6.008
Wikipedia 5.982
Rodwell 5.97
Grimsley 6.148
Solomon 5.982
Lehninger 5.982
Nozaki 6.249
DTASelect 6.427
Thurlkill 6.427
EMBOSS 6.389
Sillero 6.351
Patrickios 3.973
IPC_peptide 6.008
IPC2_peptide 6.338
IPC2.peptide.svr19 6.312
Protein with the highest isoelectric point:
>tr|A0A1L3KIM5|A0A1L3KIM5_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 2 OX=1923502 PE=4 SV=1
MM1 pKa = 7.58 SPRR4 pKa = 11.84 NLFPSYY10 pKa = 11.42 GDD12 pKa = 3.34 GCSSVNSTEE21 pKa = 3.85 NTLASILEE29 pKa = 4.65 SPFHH33 pKa = 6.14 PAWSAVVEE41 pKa = 4.36 GFFGNEE47 pKa = 3.95 VVTDD51 pKa = 3.47 THH53 pKa = 7.85 VDD55 pKa = 3.26 EE56 pKa = 4.57 LAIYY60 pKa = 10.4 EE61 pKa = 4.8 DD62 pKa = 4.39 GLDD65 pKa = 3.47 LSAYY69 pKa = 7.12 STSSVEE75 pKa = 4.01 QVVRR79 pKa = 11.84 CCEE82 pKa = 4.2 VYY84 pKa = 10.92 SFIYY88 pKa = 9.81 DD89 pKa = 3.18 QYY91 pKa = 10.67 GYY93 pKa = 11.09 KK94 pKa = 10.37 FLFSPTDD101 pKa = 3.15 QRR103 pKa = 11.84 LWLEE107 pKa = 4.67 LGDD110 pKa = 4.3 KK111 pKa = 10.78 DD112 pKa = 5.12 LLEE115 pKa = 5.36 VYY117 pKa = 9.85 LKK119 pKa = 10.39 WITANILAAVWEE131 pKa = 4.05 QDD133 pKa = 3.3 EE134 pKa = 4.99 LVPLPSSLLDD144 pKa = 3.94 FSARR148 pKa = 11.84 FPAFGLTTRR157 pKa = 11.84 DD158 pKa = 3.23 DD159 pKa = 3.68 RR160 pKa = 11.84 LFRR163 pKa = 11.84 SMIRR167 pKa = 11.84 SSRR170 pKa = 11.84 RR171 pKa = 11.84 SKK173 pKa = 9.1 KK174 pKa = 6.89 QQRR177 pKa = 11.84 FVYY180 pKa = 9.7 TLYY183 pKa = 10.45 QGKK186 pKa = 9.96 AGCNAMRR193 pKa = 11.84 PEE195 pKa = 4.09 LVAAKK200 pKa = 9.9 VVSAVEE206 pKa = 4.11 TLATPPEE213 pKa = 4.46 GEE215 pKa = 4.06 EE216 pKa = 4.15 SIGILGHH223 pKa = 7.43 LITKK227 pKa = 10.06 EE228 pKa = 4.06 DD229 pKa = 3.26 IRR231 pKa = 11.84 GQLLRR236 pKa = 11.84 TVTEE240 pKa = 4.35 IYY242 pKa = 9.18 GTGHH246 pKa = 5.11 ARR248 pKa = 11.84 EE249 pKa = 3.96 RR250 pKa = 11.84 RR251 pKa = 11.84 TPGSRR256 pKa = 11.84 RR257 pKa = 11.84 MASSKK262 pKa = 11.08 SSFQYY267 pKa = 9.93 TRR269 pKa = 11.84 SKK271 pKa = 11.11 GGAHH275 pKa = 6.73 EE276 pKa = 4.31 FLVDD280 pKa = 3.54 NAPASMFIQPRR291 pKa = 11.84 ILSGFVVYY299 pKa = 8.69 KK300 pKa = 8.71 TQVRR304 pKa = 11.84 PYY306 pKa = 8.49 YY307 pKa = 8.73 TTFQAEE313 pKa = 4.31 DD314 pKa = 3.39 WEE316 pKa = 4.39 EE317 pKa = 3.84 LQEE320 pKa = 4.11 LSRR323 pKa = 11.84 RR324 pKa = 11.84 AAWSEE329 pKa = 3.74 EE330 pKa = 3.74 MDD332 pKa = 4.54 CYY334 pKa = 10.86 PVGLPEE340 pKa = 4.1 AFKK343 pKa = 11.24 VRR345 pKa = 11.84 VITKK349 pKa = 10.1 GAVHH353 pKa = 7.27 HH354 pKa = 6.45 YY355 pKa = 9.81 NLARR359 pKa = 11.84 RR360 pKa = 11.84 WQPAMWEE367 pKa = 4.08 PLANHH372 pKa = 6.36 PTFEE376 pKa = 4.87 LVGTPNCQRR385 pKa = 11.84 IMNRR389 pKa = 11.84 FLAKK393 pKa = 10.09 CDD395 pKa = 4.01 PDD397 pKa = 3.93 DD398 pKa = 5.29 DD399 pKa = 4.47 RR400 pKa = 11.84 AFTSGDD406 pKa = 3.42 YY407 pKa = 11.15 ASATDD412 pKa = 4.39 YY413 pKa = 11.46 LDD415 pKa = 4.3 SDD417 pKa = 4.42 LSRR420 pKa = 11.84 SCLEE424 pKa = 4.05 YY425 pKa = 10.13 VCNALRR431 pKa = 11.84 VPFEE435 pKa = 4.14 DD436 pKa = 3.52 TVILAEE442 pKa = 4.33 ALTDD446 pKa = 3.63 HH447 pKa = 7.06 RR448 pKa = 11.84 LHH450 pKa = 6.47 YY451 pKa = 10.4 VDD453 pKa = 4.94 SEE455 pKa = 4.21 GVEE458 pKa = 3.9 QVKK461 pKa = 7.79 QQRR464 pKa = 11.84 RR465 pKa = 11.84 GQLMGSPISFPILCLFNAALTRR487 pKa = 11.84 YY488 pKa = 8.88 ALEE491 pKa = 4.16 IASCDD496 pKa = 3.65 SVALDD501 pKa = 5.36 LEE503 pKa = 4.59 DD504 pKa = 6.02 LPMLINGDD512 pKa = 4.11 DD513 pKa = 4.02 LLCRR517 pKa = 11.84 TNPLEE522 pKa = 3.87 YY523 pKa = 10.49 LVWKK527 pKa = 10.26 DD528 pKa = 3.07 IVNFGGLTPSIGKK541 pKa = 8.67 NFRR544 pKa = 11.84 HH545 pKa = 5.35 RR546 pKa = 11.84 TIGTINSEE554 pKa = 3.5 MWTFKK559 pKa = 9.85 HH560 pKa = 6.32 TIHH563 pKa = 6.83 GCPANGTDD571 pKa = 2.94 TAFYY575 pKa = 9.61 QGKK578 pKa = 8.26 RR579 pKa = 11.84 QPIIEE584 pKa = 4.39 IGLVRR589 pKa = 11.84 GSVKK593 pKa = 10.44 RR594 pKa = 11.84 GTINRR599 pKa = 11.84 NVDD602 pKa = 3.49 EE603 pKa = 5.25 LSPFVDD609 pKa = 3.08 SSKK612 pKa = 10.53 FGKK615 pKa = 10.41 SKK617 pKa = 8.53 EE618 pKa = 4.12 QCWNRR623 pKa = 11.84 FLDD626 pKa = 3.67 TCPNRR631 pKa = 11.84 LTAYY635 pKa = 10.0 DD636 pKa = 3.87 FLWRR640 pKa = 11.84 SVSSDD645 pKa = 2.73 ILEE648 pKa = 4.23 SLPRR652 pKa = 11.84 GMPMCCPTWLGGAGFPLPPLGHH674 pKa = 7.28 ALRR677 pKa = 11.84 DD678 pKa = 3.57 RR679 pKa = 11.84 RR680 pKa = 11.84 EE681 pKa = 3.58 PSAYY685 pKa = 9.47 QRR687 pKa = 11.84 LLARR691 pKa = 11.84 YY692 pKa = 9.27 LYY694 pKa = 8.96 DD695 pKa = 3.06 TYY697 pKa = 11.46 GYY699 pKa = 10.71 GLGKK703 pKa = 10.1 RR704 pKa = 11.84 YY705 pKa = 10.64 VSMLEE710 pKa = 4.19 TGSLPANLVKK720 pKa = 10.59 EE721 pKa = 4.32 MNTDD725 pKa = 2.9 NGIRR729 pKa = 11.84 DD730 pKa = 3.68 VLGVPKK736 pKa = 9.91 PVPEE740 pKa = 4.51 SADD743 pKa = 3.38 SVDD746 pKa = 4.21 GIKK749 pKa = 11.04 GNLHH753 pKa = 5.6 SEE755 pKa = 4.27 KK756 pKa = 10.24 TPLTPLPQTHH766 pKa = 6.83 FFMCGLINHH775 pKa = 6.21 TEE777 pKa = 4.04 EE778 pKa = 4.22 EE779 pKa = 4.43 STGRR783 pKa = 11.84 FSCEE787 pKa = 3.08 KK788 pKa = 9.17 MYY790 pKa = 11.24 SRR792 pKa = 11.84 LQHH795 pKa = 6.56 KK796 pKa = 9.41 ALKK799 pKa = 9.78 HH800 pKa = 4.86 GRR802 pKa = 11.84 GPLDD806 pKa = 3.56 CKK808 pKa = 10.67 FFLPTPPSLKK818 pKa = 10.17 LEE820 pKa = 4.13 SYY822 pKa = 10.19 VFQEE826 pKa = 4.43 VVAA829 pKa = 4.65
Molecular weight: 93.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.67
IPC2_protein 5.703
IPC_protein 5.741
Toseland 6.046
ProMoST 6.084
Dawson 5.982
Bjellqvist 6.008
Wikipedia 5.982
Rodwell 5.97
Grimsley 6.148
Solomon 5.982
Lehninger 5.982
Nozaki 6.249
DTASelect 6.427
Thurlkill 6.427
EMBOSS 6.389
Sillero 6.351
Patrickios 3.973
IPC_peptide 6.008
IPC2_peptide 6.338
IPC2.peptide.svr19 6.312
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
829
829
829
829.0
93.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.273 ± 0.0
2.413 ± 0.0
5.669 ± 0.0
6.514 ± 0.0
4.704 ± 0.0
6.755 ± 0.0
2.292 ± 0.0
3.981 ± 0.0
3.981 ± 0.0
10.374 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.051 ± 0.0
3.378 ± 0.0
6.031 ± 0.0
3.136 ± 0.0
6.876 ± 0.0
7.841 ± 0.0
6.031 ± 0.0
6.152 ± 0.0
1.568 ± 0.0
3.981 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here