Euzebyella marina

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Euzebyella

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3952 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2L9B8|A0A3G2L9B8_9FLAO Putative fluoride ion transporter CrcB OS=Euzebyella marina OX=1761453 GN=crcB PE=3 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84FLLALSLVLLFGLQSQAQNSPDD24 pKa = 3.7CRR26 pKa = 11.84SAIPVCADD34 pKa = 3.11APIMGRR40 pKa = 11.84ADD42 pKa = 3.34GGGDD46 pKa = 3.23IDD48 pKa = 5.85DD49 pKa = 5.34FDD51 pKa = 5.93PEE53 pKa = 4.66VITEE57 pKa = 4.4TGCLEE62 pKa = 4.24KK63 pKa = 11.09GSVSNANIEE72 pKa = 4.6NNTSWYY78 pKa = 9.78VFRR81 pKa = 11.84AGTGGQVGFDD91 pKa = 3.54IEE93 pKa = 4.71ALPLPGEE100 pKa = 4.31TTITAEE106 pKa = 3.5WDD108 pKa = 3.61FAVYY112 pKa = 10.33GPDD115 pKa = 3.48VACGEE120 pKa = 4.24ISSGQAQPIRR130 pKa = 11.84CNYY133 pKa = 7.81EE134 pKa = 3.97VNGTRR139 pKa = 11.84YY140 pKa = 8.26TGIGVNPEE148 pKa = 4.11DD149 pKa = 4.1GQAGVPSTTQNLNTYY164 pKa = 9.36DD165 pKa = 3.25EE166 pKa = 4.57WLDD169 pKa = 3.61VLPGEE174 pKa = 4.81VYY176 pKa = 10.98YY177 pKa = 10.27ILINNFSNNTNNDD190 pKa = 3.41PEE192 pKa = 4.34EE193 pKa = 4.78FILTFTGNSVEE204 pKa = 4.38ADD206 pKa = 3.15QNTALDD212 pKa = 3.73CTLRR216 pKa = 11.84DD217 pKa = 3.55EE218 pKa = 5.64FLGLDD223 pKa = 3.36INACEE228 pKa = 4.27GDD230 pKa = 3.67PDD232 pKa = 3.97IEE234 pKa = 4.12LVALNSPAGPDD245 pKa = 3.12IANVAWSVDD254 pKa = 3.85FDD256 pKa = 5.18DD257 pKa = 6.49DD258 pKa = 4.1GTIDD262 pKa = 3.51QPMGSGPTATHH273 pKa = 6.29LTVSSPNSGRR283 pKa = 11.84YY284 pKa = 8.39FVDD287 pKa = 2.61ITTSEE292 pKa = 4.24GAHH295 pKa = 6.08YY296 pKa = 10.62LDD298 pKa = 6.12DD299 pKa = 4.34ILITFYY305 pKa = 9.84GTPVLDD311 pKa = 3.64RR312 pKa = 11.84VEE314 pKa = 4.45LLNSNLTLDD323 pKa = 3.82PDD325 pKa = 3.89RR326 pKa = 11.84NNIEE330 pKa = 4.05IFVDD334 pKa = 3.46GDD336 pKa = 3.15GDD338 pKa = 3.64YY339 pKa = 10.89EE340 pKa = 4.28YY341 pKa = 10.95AVNGGEE347 pKa = 4.09FQDD350 pKa = 4.47DD351 pKa = 4.26SVFNDD356 pKa = 3.48VPPGINTLVINDD368 pKa = 3.92KK369 pKa = 10.55NGCGTTEE376 pKa = 4.52PIEE379 pKa = 4.07FLVVGYY385 pKa = 9.34PKK387 pKa = 10.75FFTPNNDD394 pKa = 3.4GANDD398 pKa = 3.16TWNIKK403 pKa = 10.53GIEE406 pKa = 4.14TLNNPVVFVFDD417 pKa = 4.47RR418 pKa = 11.84YY419 pKa = 10.88GKK421 pKa = 10.6LLAQLNSNTEE431 pKa = 3.24WDD433 pKa = 3.32GTYY436 pKa = 10.43NGKK439 pKa = 8.84PLPSSDD445 pKa = 2.58YY446 pKa = 8.79WFRR449 pKa = 11.84FEE451 pKa = 4.86YY452 pKa = 10.89EE453 pKa = 3.76EE454 pKa = 4.94DD455 pKa = 3.65EE456 pKa = 4.58EE457 pKa = 5.11GVVVAKK463 pKa = 8.16STRR466 pKa = 11.84ANFTLKK472 pKa = 10.46RR473 pKa = 3.76

Molecular weight: 51.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2L9J9|A0A3G2L9J9_9FLAO Cysteine synthase family protein OS=Euzebyella marina OX=1761453 GN=D1013_16855 PE=4 SV=1
MM1 pKa = 7.26LRR3 pKa = 11.84WTVIFVILAIVAAIFGFGGIAAGAASIAKK32 pKa = 9.38ILFFIFLVLFVISLFMGRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84II53 pKa = 3.67

Molecular weight: 5.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3952

0

3952

1409240

38

5361

356.6

40.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.486 ± 0.035

0.727 ± 0.014

5.893 ± 0.053

6.981 ± 0.046

5.046 ± 0.03

6.884 ± 0.047

1.858 ± 0.022

7.134 ± 0.031

7.035 ± 0.074

9.421 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.308 ± 0.023

5.732 ± 0.041

3.611 ± 0.024

3.479 ± 0.02

3.792 ± 0.029

6.61 ± 0.033

5.51 ± 0.054

6.371 ± 0.031

1.163 ± 0.016

3.96 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski