Porcine serum associated circular DNA virus 2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385FPN9|A0A385FPN9_9VIRU Putative capsid protein OS=Porcine serum associated circular DNA virus 2 OX=2517212 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84LKK4 pKa = 11.0NNEE7 pKa = 4.0EE8 pKa = 4.15LKK10 pKa = 10.97NDD12 pKa = 4.94LIALKK17 pKa = 9.41TCDD20 pKa = 3.67YY21 pKa = 11.02LLSCEE26 pKa = 4.47EE27 pKa = 3.98EE28 pKa = 4.39APTTKK33 pKa = 10.04HH34 pKa = 4.98KK35 pKa = 10.39HH36 pKa = 3.49IHH38 pKa = 6.0IYY40 pKa = 9.59VHH42 pKa = 6.67FSQPYY47 pKa = 8.02RR48 pKa = 11.84LSKK51 pKa = 10.64KK52 pKa = 9.7ILNHH56 pKa = 6.18KK57 pKa = 9.92AHH59 pKa = 6.94IEE61 pKa = 3.82ICRR64 pKa = 11.84GSPKK68 pKa = 10.63EE69 pKa = 3.79NIAYY73 pKa = 9.47IEE75 pKa = 4.27KK76 pKa = 10.34DD77 pKa = 3.14GHH79 pKa = 7.18ILDD82 pKa = 5.34EE83 pKa = 4.99IGEE86 pKa = 4.27RR87 pKa = 11.84PRR89 pKa = 11.84QGAKK93 pKa = 7.73TVKK96 pKa = 9.16EE97 pKa = 4.01LKK99 pKa = 10.21EE100 pKa = 3.87ADD102 pKa = 3.47INDD105 pKa = 4.14IDD107 pKa = 3.67PHH109 pKa = 7.99LYY111 pKa = 10.35NIKK114 pKa = 10.56CKK116 pKa = 10.03IDD118 pKa = 4.01DD119 pKa = 3.68KK120 pKa = 11.22TKK122 pKa = 10.86EE123 pKa = 4.01EE124 pKa = 4.0EE125 pKa = 3.9QFLNMLEE132 pKa = 4.46EE133 pKa = 4.21IEE135 pKa = 4.95KK136 pKa = 10.85DD137 pKa = 3.53DD138 pKa = 4.63LKK140 pKa = 11.14GPEE143 pKa = 4.39IIYY146 pKa = 10.27ICGDD150 pKa = 3.22SGKK153 pKa = 10.78GKK155 pKa = 8.57TYY157 pKa = 10.46KK158 pKa = 10.25AYY160 pKa = 9.85KK161 pKa = 9.39IALSKK166 pKa = 10.46FDD168 pKa = 4.3KK169 pKa = 10.85KK170 pKa = 11.02DD171 pKa = 3.11IGKK174 pKa = 8.47LTIEE178 pKa = 4.29NNFIQITNDD187 pKa = 2.98KK188 pKa = 9.11AKK190 pKa = 10.28CYY192 pKa = 10.21VIEE195 pKa = 4.38EE196 pKa = 4.3FRR198 pKa = 11.84SSQIKK203 pKa = 9.76AASFLQLTDD212 pKa = 3.22KK213 pKa = 11.26YY214 pKa = 11.22GYY216 pKa = 7.66TCNIKK221 pKa = 10.7GSFKK225 pKa = 10.63SIRR228 pKa = 11.84PEE230 pKa = 4.12CIIIASIIKK239 pKa = 10.15PEE241 pKa = 3.73EE242 pKa = 4.05LYY244 pKa = 11.06QNEE247 pKa = 4.63EE248 pKa = 3.7INKK251 pKa = 8.72QFTRR255 pKa = 11.84RR256 pKa = 11.84ITQIINLGYY265 pKa = 10.62DD266 pKa = 3.23EE267 pKa = 5.98EE268 pKa = 4.38EE269 pKa = 4.65DD270 pKa = 3.71KK271 pKa = 11.54NKK273 pKa = 10.7YY274 pKa = 10.15IYY276 pKa = 10.81DD277 pKa = 4.73DD278 pKa = 4.17DD279 pKa = 4.25LL280 pKa = 6.43

Molecular weight: 32.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385FPN9|A0A385FPN9_9VIRU Putative capsid protein OS=Porcine serum associated circular DNA virus 2 OX=2517212 PE=4 SV=1
MM1 pKa = 7.1VFYY4 pKa = 10.48GARR7 pKa = 11.84KK8 pKa = 7.71NRR10 pKa = 11.84RR11 pKa = 11.84YY12 pKa = 10.03NRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84NSRR19 pKa = 11.84LSTRR23 pKa = 11.84RR24 pKa = 11.84IYY26 pKa = 11.14SRR28 pKa = 11.84TSAKK32 pKa = 10.37SQASQIAALRR42 pKa = 11.84NRR44 pKa = 11.84INKK47 pKa = 8.49VYY49 pKa = 10.03KK50 pKa = 9.41VCKK53 pKa = 9.81PEE55 pKa = 3.48IKK57 pKa = 9.87TIVTSAEE64 pKa = 3.96TMPYY68 pKa = 9.51TSQTGSSYY76 pKa = 10.85YY77 pKa = 10.14RR78 pKa = 11.84FYY80 pKa = 11.2PMTSPDD86 pKa = 4.44LGTGDD91 pKa = 4.28KK92 pKa = 11.03DD93 pKa = 4.14RR94 pKa = 11.84IGNFIRR100 pKa = 11.84VINGVLYY107 pKa = 9.94LSCEE111 pKa = 4.45YY112 pKa = 10.68YY113 pKa = 10.58NSSATGYY120 pKa = 10.61HH121 pKa = 4.84NTEE124 pKa = 3.61SSGAQVRR131 pKa = 11.84VVIGQYY137 pKa = 9.24KK138 pKa = 7.02QHH140 pKa = 6.71IGAGTVPNIDD150 pKa = 3.88EE151 pKa = 4.71LFEE154 pKa = 4.55FPSNTGANYY163 pKa = 8.98TQMALSPLLEE173 pKa = 5.21GITNKK178 pKa = 10.35YY179 pKa = 10.46NILKK183 pKa = 10.24DD184 pKa = 3.61LRR186 pKa = 11.84FTLTTDD192 pKa = 3.76RR193 pKa = 11.84NQKK196 pKa = 7.66MLKK199 pKa = 9.9IPFKK203 pKa = 10.56PIAPYY208 pKa = 10.58VFNDD212 pKa = 3.08EE213 pKa = 4.84GLFPNCWACLVVTGLHH229 pKa = 6.45YY230 pKa = 10.05DD231 pKa = 3.34TDD233 pKa = 3.85FTEE236 pKa = 4.59TVKK239 pKa = 9.59ITVSDD244 pKa = 3.56KK245 pKa = 11.38LVFTDD250 pKa = 3.83AA251 pKa = 4.41

Molecular weight: 28.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

531

251

280

265.5

30.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.896 ± 0.415

2.26 ± 0.405

6.026 ± 1.0

7.345 ± 2.287

3.578 ± 0.489

5.085 ± 0.542

2.072 ± 0.533

9.981 ± 2.195

9.793 ± 2.322

7.156 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.318 ± 0.41

6.215 ± 0.34

3.578 ± 0.489

3.202 ± 0.009

5.085 ± 1.27

5.838 ± 1.054

6.78 ± 1.693

3.578 ± 1.701

0.188 ± 0.128

6.026 ± 0.454
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski