Arthrobacter phage Powerpuff

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Yangvirus; unclassified Yangvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9JC82|A0A6B9JC82_9CAUD Uncharacterized protein OS=Arthrobacter phage Powerpuff OX=2686083 GN=51 PE=4 SV=1
MM1 pKa = 7.84LEE3 pKa = 4.3YY4 pKa = 10.84LDD6 pKa = 5.91DD7 pKa = 5.77DD8 pKa = 5.26PDD10 pKa = 5.47LEE12 pKa = 5.09LDD14 pKa = 3.26PRR16 pKa = 11.84EE17 pKa = 4.38YY18 pKa = 11.11VCEE21 pKa = 4.2TCRR24 pKa = 11.84LTHH27 pKa = 6.06WRR29 pKa = 11.84GAPDD33 pKa = 3.65PCDD36 pKa = 3.41RR37 pKa = 11.84SS38 pKa = 3.51

Molecular weight: 4.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J8X3|A0A6B9J8X3_9CAUD DNA primase/helicase OS=Arthrobacter phage Powerpuff OX=2686083 GN=40 PE=4 SV=1
MM1 pKa = 7.37GLVAALIWAGIVSFGPVHH19 pKa = 7.06LDD21 pKa = 3.46FWTEE25 pKa = 3.5LGLIFIGAFILGALGAAAEE44 pKa = 4.27IMRR47 pKa = 11.84AILPFMGTRR56 pKa = 11.84RR57 pKa = 11.84SGKK60 pKa = 8.66IAEE63 pKa = 4.34VLAVQHH69 pKa = 6.1FRR71 pKa = 11.84QKK73 pKa = 11.2AVIEE77 pKa = 4.62AGTGSTPGPRR87 pKa = 11.84IQRR90 pKa = 11.84PAGVAKK96 pKa = 10.29PLTAAA101 pKa = 4.22

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13480

36

990

192.6

20.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.613 ± 0.569

0.49 ± 0.084

6.424 ± 0.337

6.513 ± 0.386

3.071 ± 0.185

8.272 ± 0.439

1.869 ± 0.154

4.392 ± 0.258

4.28 ± 0.269

8.539 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.136

2.292 ± 0.234

5.096 ± 0.28

2.545 ± 0.212

7.359 ± 0.519

5.371 ± 0.218

6.543 ± 0.325

7.077 ± 0.274

1.766 ± 0.125

2.619 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski