Campylobacter virus NCTC12673
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4YAI3|F4YAI3_9CAUD Ribonucleoside-diphosphate reductase OS=Campylobacter virus NCTC12673 OX=934027 PE=3 SV=1
MM1 pKa = 7.24 IQFNLLCSYY10 pKa = 10.7 VIFSSITCVIFNDD23 pKa = 3.18 IDD25 pKa = 3.43 NRR27 pKa = 11.84 EE28 pKa = 4.12 YY29 pKa = 11.15 EE30 pKa = 4.04 NMIVEE35 pKa = 5.41 FISIFIFFIFFVYY48 pKa = 10.31 QLIIYY53 pKa = 10.3 RR54 pKa = 11.84 NFNRR58 pKa = 11.84 DD59 pKa = 3.09 KK60 pKa = 11.72 VEE62 pKa = 5.33 DD63 pKa = 3.92 NLCC66 pKa = 3.85
Molecular weight: 8.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.125
IPC2_protein 4.38
IPC_protein 4.151
Toseland 3.973
ProMoST 4.304
Dawson 4.126
Bjellqvist 4.291
Wikipedia 4.05
Rodwell 3.986
Grimsley 3.897
Solomon 4.101
Lehninger 4.062
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.037
EMBOSS 4.062
Sillero 4.266
Patrickios 0.401
IPC_peptide 4.101
IPC2_peptide 4.253
IPC2.peptide.svr19 4.175
Protein with the highest isoelectric point:
>tr|F4YA75|F4YA75_9CAUD Gp62 clamp loader subunit OS=Campylobacter virus NCTC12673 OX=934027 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 9.96 RR3 pKa = 11.84 DD4 pKa = 3.31 GSIIKK9 pKa = 10.08 SFKK12 pKa = 10.65 RR13 pKa = 11.84 EE14 pKa = 3.62 INLQTRR20 pKa = 11.84 FIKK23 pKa = 10.85 NKK25 pKa = 8.46 TKK27 pKa = 8.49 YY28 pKa = 7.92 TRR30 pKa = 11.84 KK31 pKa = 9.49 EE32 pKa = 3.71 KK33 pKa = 10.38 HH34 pKa = 6.19 KK35 pKa = 10.51 KK36 pKa = 8.96 GAINGFNN43 pKa = 3.3
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 9.999
IPC_protein 10.438
Toseland 11.199
ProMoST 10.774
Dawson 11.242
Bjellqvist 10.877
Wikipedia 11.403
Rodwell 11.754
Grimsley 11.257
Solomon 11.359
Lehninger 11.345
Nozaki 11.155
DTASelect 10.877
Thurlkill 11.169
EMBOSS 11.594
Sillero 11.169
Patrickios 11.506
IPC_peptide 11.374
IPC2_peptide 9.355
IPC2.peptide.svr19 8.535
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
166
0
166
40602
35
1365
244.6
28.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.256 ± 0.174
1.877 ± 0.15
6.28 ± 0.191
6.81 ± 0.192
5.027 ± 0.147
4.983 ± 0.15
1.566 ± 0.092
9.359 ± 0.175
10.187 ± 0.221
8.741 ± 0.144
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.118 ± 0.08
8.63 ± 0.175
2.579 ± 0.091
2.628 ± 0.09
2.68 ± 0.094
6.879 ± 0.161
5.167 ± 0.196
5.12 ± 0.142
0.825 ± 0.048
5.288 ± 0.128
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here