Clostridium sp. CAG:793

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1163 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5NPQ0|R5NPQ0_9CLOT Phosphoesterase RecJ domain protein OS=Clostridium sp. CAG:793 OX=1262840 GN=BN786_00331 PE=4 SV=1
MM1 pKa = 8.32DD2 pKa = 6.03DD3 pKa = 4.98LNNNEE8 pKa = 4.21NNQNNQAGYY17 pKa = 10.23YY18 pKa = 8.66PNNSQDD24 pKa = 3.52PYY26 pKa = 10.31SANSGFGPVNPYY38 pKa = 9.76MNNQDD43 pKa = 2.88GSSFNPYY50 pKa = 9.78GMADD54 pKa = 3.97SNSMPQNPYY63 pKa = 9.83QNSGEE68 pKa = 4.08FNPYY72 pKa = 10.13SLYY75 pKa = 10.9NSTYY79 pKa = 10.03PGAEE83 pKa = 3.87NNQNTVQTSDD93 pKa = 3.15NSHH96 pKa = 7.23DD97 pKa = 3.73SNYY100 pKa = 10.18AQNPNTTVEE109 pKa = 4.1NDD111 pKa = 3.07NGTFSPFDD119 pKa = 3.8LSNEE123 pKa = 3.89SSAVPEE129 pKa = 5.0QSTQMQNNQFNHH141 pKa = 5.31VDD143 pKa = 3.22ASNNQAQTEE152 pKa = 4.11PMQEE156 pKa = 3.59NQMPQEE162 pKa = 4.29PVQEE166 pKa = 4.01NQIPQEE172 pKa = 4.37PIQGNATSYY181 pKa = 11.43DD182 pKa = 3.49PAYY185 pKa = 10.56EE186 pKa = 4.07NMQQQFVQNQGQIRR200 pKa = 11.84NPILSQGLPTDD211 pKa = 4.63DD212 pKa = 4.93GMNNYY217 pKa = 7.87QQNSSFSNNIDD228 pKa = 3.38SYY230 pKa = 10.48NTQPSQDD237 pKa = 3.48YY238 pKa = 10.57SFNGSDD244 pKa = 3.31YY245 pKa = 11.2TMEE248 pKa = 4.45FVKK251 pKa = 10.77AWMGNLYY258 pKa = 10.43EE259 pKa = 4.62KK260 pKa = 10.84AHH262 pKa = 5.77SKK264 pKa = 9.92KK265 pKa = 10.48FNWPAAIFGGIYY277 pKa = 9.59FLYY280 pKa = 10.38RR281 pKa = 11.84KK282 pKa = 9.05MYY284 pKa = 8.73LTGILLIMFSSILTFLMLFLSVKK307 pKa = 10.31VGAGLMALLPVINIVLLLVYY327 pKa = 10.51GFGFYY332 pKa = 10.47PMYY335 pKa = 10.73RR336 pKa = 11.84NFVRR340 pKa = 11.84GKK342 pKa = 9.38LNKK345 pKa = 9.52YY346 pKa = 9.43KK347 pKa = 9.33QTITDD352 pKa = 3.48NSQLVQTASSKK363 pKa = 11.17GGTSPLAVILCCIVVPIISSLVLTLLATVGLFNIFKK399 pKa = 10.39NISGNINTPSNIVSDD414 pKa = 3.71NSIIEE419 pKa = 4.92DD420 pKa = 3.61NTDD423 pKa = 2.98MKK425 pKa = 11.18SYY427 pKa = 9.81TFLNQYY433 pKa = 9.14TIDD436 pKa = 4.02YY437 pKa = 10.74DD438 pKa = 3.76SLTWFFNQSDD448 pKa = 3.91SSLTKK453 pKa = 10.29GDD455 pKa = 3.62YY456 pKa = 10.64KK457 pKa = 10.43LTYY460 pKa = 9.41SGQSIPDD467 pKa = 3.62LKK469 pKa = 11.24SSFNVDD475 pKa = 2.58STTPAGRR482 pKa = 11.84SSLLSTLMSSLQAQAASMNLTVDD505 pKa = 3.25VGTSNFVLGVKK516 pKa = 10.05NYY518 pKa = 9.86YY519 pKa = 10.72AYY521 pKa = 9.9IDD523 pKa = 3.65VASNNTLTRR532 pKa = 11.84YY533 pKa = 9.94YY534 pKa = 10.04IILIPEE540 pKa = 4.52DD541 pKa = 5.38DD542 pKa = 4.07ILFQFTLEE550 pKa = 4.32TNDD553 pKa = 3.3TSIDD557 pKa = 3.55YY558 pKa = 7.66ATNIEE563 pKa = 4.36IVNMLTNISTEE574 pKa = 4.15SSDD577 pKa = 5.1EE578 pKa = 4.02EE579 pKa = 5.81ADD581 pKa = 4.78DD582 pKa = 3.85NTISNDD588 pKa = 3.53TVISNDD594 pKa = 3.06VSDD597 pKa = 4.3ILSNTPVNEE606 pKa = 3.93VTSARR611 pKa = 11.84NTISNTNSLSDD622 pKa = 3.52MLNN625 pKa = 3.08

Molecular weight: 69.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5NP83|R5NP83_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:793 OX=1262840 GN=BN786_00161 PE=4 SV=1
MM1 pKa = 7.28TFVFSIACAVAAVQILLSLALVFHH25 pKa = 6.67KK26 pKa = 10.52HH27 pKa = 4.73NVLAIVSEE35 pKa = 4.41LATSTGLTLFISRR48 pKa = 11.84YY49 pKa = 8.96LLAWPLPYY57 pKa = 9.87IILLGGAEE65 pKa = 4.56YY66 pKa = 8.86ITVKK70 pKa = 10.53IIILLVIVILKK81 pKa = 8.93LRR83 pKa = 11.84CFTVLTILTHH93 pKa = 5.72PSNRR97 pKa = 11.84RR98 pKa = 11.84YY99 pKa = 9.88SVYY102 pKa = 10.2KK103 pKa = 9.87KK104 pKa = 9.76RR105 pKa = 11.84YY106 pKa = 7.0SGRR109 pKa = 11.84MHH111 pKa = 7.65FISMVYY117 pKa = 9.4RR118 pKa = 11.84SKK120 pKa = 10.78YY121 pKa = 9.62HH122 pKa = 6.35PRR124 pKa = 11.84LKK126 pKa = 10.55LGMPAMVGKK135 pKa = 10.22YY136 pKa = 10.12SNHH139 pKa = 5.95TGIKK143 pKa = 9.08FDD145 pKa = 3.49KK146 pKa = 10.83KK147 pKa = 10.14GFPVFKK153 pKa = 10.16SICTITLPRR162 pKa = 11.84KK163 pKa = 9.46YY164 pKa = 9.88RR165 pKa = 11.84YY166 pKa = 6.9QTRR169 pKa = 11.84DD170 pKa = 3.0VHH172 pKa = 6.41FRR174 pKa = 11.84CASKK178 pKa = 10.74LLYY181 pKa = 10.03KK182 pKa = 10.29RR183 pKa = 11.84AVSNRR188 pKa = 11.84YY189 pKa = 8.48VRR191 pKa = 11.84CKK193 pKa = 8.63FTKK196 pKa = 10.35SEE198 pKa = 4.13LATFKK203 pKa = 11.16CGDD206 pKa = 3.75VPSKK210 pKa = 9.16YY211 pKa = 8.36TWHH214 pKa = 6.52HH215 pKa = 5.59HH216 pKa = 4.36QSAGVLQLVDD226 pKa = 3.91RR227 pKa = 11.84KK228 pKa = 9.29VHH230 pKa = 6.52ANTPHH235 pKa = 6.42IGGYY239 pKa = 8.63SIWGADD245 pKa = 3.21NN246 pKa = 3.73

Molecular weight: 28.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1163

0

1163

367022

29

4295

315.6

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.521 ± 0.067

1.127 ± 0.024

5.797 ± 0.049

7.694 ± 0.097

3.669 ± 0.062

5.793 ± 0.078

1.253 ± 0.026

10.106 ± 0.12

9.575 ± 0.083

7.955 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.045

6.837 ± 0.078

2.657 ± 0.044

2.943 ± 0.041

3.436 ± 0.058

6.2 ± 0.088

5.721 ± 0.179

6.088 ± 0.06

0.638 ± 0.024

4.45 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski