Rhodanobacter sp. FW510-R10
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3386 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A154R534|A0A154R534_9GAMM Tol-Pal system protein TolR OS=Rhodanobacter sp. FW510-R10 OX=1524462 GN=tolR PE=3 SV=1
MM1 pKa = 7.04 SQSSTEE7 pKa = 3.75 AAALRR12 pKa = 11.84 KK13 pKa = 8.44 WMCVVCGYY21 pKa = 10.35 IYY23 pKa = 10.72 DD24 pKa = 4.06 EE25 pKa = 4.46 AAGVPDD31 pKa = 3.78 EE32 pKa = 5.36 GIPAGTRR39 pKa = 11.84 WADD42 pKa = 3.79 VPDD45 pKa = 3.6 TWTCPDD51 pKa = 3.9 CGATKK56 pKa = 10.7 ADD58 pKa = 3.71 FEE60 pKa = 4.72 MIEE63 pKa = 3.88 IDD65 pKa = 3.43
Molecular weight: 7.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.872
Dawson 3.783
Bjellqvist 4.024
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.886
Protein with the highest isoelectric point:
>tr|A0A154R1P6|A0A154R1P6_9GAMM DNA repair photolyase OS=Rhodanobacter sp. FW510-R10 OX=1524462 GN=RhoFW510R10_15330 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.06 LKK11 pKa = 10.38 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATADD26 pKa = 3.35 GRR28 pKa = 11.84 KK29 pKa = 9.36 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.75 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LIPP44 pKa = 4.02
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3386
0
3386
1116988
41
2878
329.9
35.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.141 ± 0.062
0.865 ± 0.014
5.678 ± 0.033
5.053 ± 0.038
3.348 ± 0.026
8.586 ± 0.044
2.507 ± 0.019
4.136 ± 0.027
2.885 ± 0.031
11.13 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.201 ± 0.02
2.523 ± 0.035
5.357 ± 0.03
3.838 ± 0.025
7.503 ± 0.052
5.054 ± 0.037
4.812 ± 0.038
7.382 ± 0.036
1.543 ± 0.019
2.458 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here