Flavobacterium alvei

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5AB32|A0A2S5AB32_9FLAO Uncharacterized protein OS=Flavobacterium alvei OX=2080416 GN=C3L50_10585 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.65KK3 pKa = 9.16MLLKK7 pKa = 10.91KK8 pKa = 10.42NGNAHH13 pKa = 6.56FFNFFLYY20 pKa = 10.46KK21 pKa = 9.37MAFLFLFFILFYY33 pKa = 10.84TKK35 pKa = 11.03GEE37 pKa = 4.1AQNKK41 pKa = 7.68RR42 pKa = 11.84NKK44 pKa = 9.91VEE46 pKa = 4.31LSSSSLTTLSAIKK59 pKa = 10.33KK60 pKa = 5.79EE61 pKa = 4.29TVVAVIGGTGPSDD74 pKa = 4.49DD75 pKa = 4.11FDD77 pKa = 6.82GDD79 pKa = 4.06GVINSVDD86 pKa = 4.32LDD88 pKa = 4.19DD89 pKa = 6.47DD90 pKa = 4.09NDD92 pKa = 5.08GILDD96 pKa = 3.77TDD98 pKa = 3.85EE99 pKa = 4.53TKK101 pKa = 10.97SDD103 pKa = 3.46IPVVSGFDD111 pKa = 3.69PIYY114 pKa = 10.19PVGSTSTTDD123 pKa = 3.2LPLGGRR129 pKa = 11.84AIAYY133 pKa = 7.67NAVSSGGVSYY143 pKa = 10.66HH144 pKa = 7.13CIMEE148 pKa = 4.12ITGKK152 pKa = 10.75GGLPPGIVNIQNNGPLTITGGVPNKK177 pKa = 10.31DD178 pKa = 3.13PYY180 pKa = 9.19VTYY183 pKa = 10.49SVKK186 pKa = 10.18FVPAAGFVLANYY198 pKa = 10.18QNTTPIIGATLTKK211 pKa = 9.54VTVVSADD218 pKa = 3.03VDD220 pKa = 3.7GSSNYY225 pKa = 9.99KK226 pKa = 10.06EE227 pKa = 4.02VTGYY231 pKa = 10.74LPSPNITKK239 pKa = 9.75PVEE242 pKa = 4.07IGTSLSPGGFTQGGGPAAASGYY264 pKa = 10.42VYY266 pKa = 10.74YY267 pKa = 10.85RR268 pKa = 11.84NNDD271 pKa = 3.03GVNPANNAEE280 pKa = 4.05LPPYY284 pKa = 10.53RR285 pKa = 11.84MTIFYY290 pKa = 7.98DD291 pKa = 3.46TFTQADD297 pKa = 4.65LVWGFTGSSSSIRR310 pKa = 11.84SAGSPTMKK318 pKa = 10.24FATIDD323 pKa = 3.26ITDD326 pKa = 3.55ANGNGFSNQFDD337 pKa = 4.54LDD339 pKa = 3.93SDD341 pKa = 4.17GDD343 pKa = 4.01GCSDD347 pKa = 3.83SNEE350 pKa = 4.16AYY352 pKa = 10.47GNSTLADD359 pKa = 3.54TNKK362 pKa = 9.99QYY364 pKa = 11.46GMNAYY369 pKa = 9.06PVGTVAPITPVDD381 pKa = 3.92TFNVTSDD388 pKa = 3.63GKK390 pKa = 10.8VVAASYY396 pKa = 10.42VIVNYY401 pKa = 10.6APVVTITAVSAPVDD415 pKa = 3.66VTNVLIGGTATFSATPNGASASATLKK441 pKa = 9.43WQQKK445 pKa = 10.33LNGSSTWTDD454 pKa = 2.74IVSGTVGGVTYY465 pKa = 10.47AISSFDD471 pKa = 3.46NSLTITGITAALGNSEE487 pKa = 4.1YY488 pKa = 10.69HH489 pKa = 6.69AVLSDD494 pKa = 3.63SGATGYY500 pKa = 8.26FVCGNKK506 pKa = 9.2TSASAKK512 pKa = 10.09LKK514 pKa = 10.04LANISPVANNDD525 pKa = 3.21VLTVNEE531 pKa = 4.36DD532 pKa = 3.03TSGTVNVVTNDD543 pKa = 2.91TDD545 pKa = 4.42ADD547 pKa = 3.95GTLDD551 pKa = 3.57ATTVDD556 pKa = 4.97LDD558 pKa = 3.85PATALIQNTFTVAGQGTYY576 pKa = 8.17TVNNLGVVTFVPVLNFNGSSSISYY600 pKa = 8.12TVNDD604 pKa = 3.44NLGLVSNVATLAVTITSVNDD624 pKa = 3.25APVAKK629 pKa = 10.43NDD631 pKa = 3.34VATTNEE637 pKa = 4.98DD638 pKa = 2.57ISATINVVTNVPGMDD653 pKa = 3.29TDD655 pKa = 3.52IDD657 pKa = 4.06GTVDD661 pKa = 3.16ATKK664 pKa = 10.79VDD666 pKa = 4.98LDD668 pKa = 3.94PATTGIQTTFTVTGQGTYY686 pKa = 8.24TVNNAGLVTFVPVLNYY702 pKa = 10.76NGIASPINYY711 pKa = 8.03TVYY714 pKa = 11.2DD715 pKa = 3.75NLGLVSNISTLTITVVAVNDD735 pKa = 3.71APVAKK740 pKa = 10.28NDD742 pKa = 3.45VVTTNEE748 pKa = 4.18DD749 pKa = 3.05TSITINVVTNALGLDD764 pKa = 3.62TDD766 pKa = 3.53VDD768 pKa = 4.22GTVDD772 pKa = 3.42AATVDD777 pKa = 4.22LDD779 pKa = 4.0LATAGIQKK787 pKa = 8.42TFTITGQGTYY797 pKa = 10.05TIDD800 pKa = 3.24NTGLVTFVPVLNYY813 pKa = 10.53NGSSTINYY821 pKa = 7.3TVNDD825 pKa = 3.62NLGLVSNVTTLTVTITPVNDD845 pKa = 3.2APVAKK850 pKa = 10.43NDD852 pKa = 3.34VATTNEE858 pKa = 4.98DD859 pKa = 2.57ISATINVVTNVPGMDD874 pKa = 3.29TDD876 pKa = 3.52IDD878 pKa = 4.06GTVDD882 pKa = 3.16ATKK885 pKa = 10.79VDD887 pKa = 4.98LDD889 pKa = 3.94PATTGIQTTFTVTGQGTYY907 pKa = 8.86TFNNAGLVTFVPVLNYY923 pKa = 10.76NGIASPINYY932 pKa = 8.03TVYY935 pKa = 11.2DD936 pKa = 3.75NLGLVSNIATLTITVVSVNDD956 pKa = 3.62APVANNDD963 pKa = 3.49TANTEE968 pKa = 3.55KK969 pKa = 9.97DD970 pKa = 3.41TNVIVSVASNDD981 pKa = 3.17TDD983 pKa = 3.31IDD985 pKa = 3.79GTINSATIDD994 pKa = 3.97LDD996 pKa = 3.83LATTGIQTTLTVAGQGTYY1014 pKa = 8.05TANANGTVTFDD1025 pKa = 4.06PLVSFAGTTTPINYY1039 pKa = 9.58IIQDD1043 pKa = 3.63NQAGVSNSATITIIVISDD1061 pKa = 3.39SDD1063 pKa = 3.98GDD1065 pKa = 4.1GVLDD1069 pKa = 4.88KK1070 pKa = 11.24IEE1072 pKa = 4.51IADD1075 pKa = 3.73GTNPNDD1081 pKa = 3.55YY1082 pKa = 10.54CDD1084 pKa = 4.08SKK1086 pKa = 11.49QEE1088 pKa = 4.09NATLEE1093 pKa = 3.97HH1094 pKa = 6.3SAAFLAADD1102 pKa = 4.33CDD1104 pKa = 4.23GDD1106 pKa = 4.36GISNKK1111 pKa = 10.18DD1112 pKa = 3.4EE1113 pKa = 4.14YY1114 pKa = 9.12GTNYY1118 pKa = 9.7GHH1120 pKa = 7.59PFDD1123 pKa = 5.63SNGNGTPDD1131 pKa = 3.27YY1132 pKa = 11.47LEE1134 pKa = 4.93ANNHH1138 pKa = 5.58NLSNSDD1144 pKa = 3.7DD1145 pKa = 3.8DD1146 pKa = 5.89LEE1148 pKa = 4.45IFNGVSPTSDD1158 pKa = 3.2DD1159 pKa = 3.46PKK1161 pKa = 11.21NNVFTIRR1168 pKa = 11.84NIEE1171 pKa = 4.09KK1172 pKa = 10.09YY1173 pKa = 9.8PNNSLHH1179 pKa = 5.91IFNRR1183 pKa = 11.84FGGLVYY1189 pKa = 10.0EE1190 pKa = 4.26AEE1192 pKa = 4.85GYY1194 pKa = 10.48GVGNKK1199 pKa = 8.49FFKK1202 pKa = 10.69GVSEE1206 pKa = 4.17GRR1208 pKa = 11.84STISPSAEE1216 pKa = 4.04LPEE1219 pKa = 4.15GTYY1222 pKa = 10.09FYY1224 pKa = 11.36ALKK1227 pKa = 9.85YY1228 pKa = 9.98VNSAGVTKK1236 pKa = 10.27EE1237 pKa = 3.61RR1238 pKa = 11.84SGYY1241 pKa = 10.77LYY1243 pKa = 10.41INRR1246 pKa = 4.49

Molecular weight: 130.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5A1R5|A0A2S5A1R5_9FLAO YceI domain-containing protein OS=Flavobacterium alvei OX=2080416 GN=C3L50_15435 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3153

0

3153

1138329

36

4741

361.0

40.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.443 ± 0.038

0.802 ± 0.014

5.247 ± 0.03

6.196 ± 0.049

5.353 ± 0.04

6.459 ± 0.044

1.576 ± 0.021

8.175 ± 0.042

8.086 ± 0.054

9.002 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.026

6.527 ± 0.047

3.476 ± 0.025

3.387 ± 0.026

3.004 ± 0.028

6.63 ± 0.042

6.063 ± 0.069

6.193 ± 0.032

1.12 ± 0.018

4.058 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski