Parasphingopyxis lamellibrachiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Parasphingopyxis

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2877 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D9FGT6|A0A3D9FGT6_9SPHN DNA helicase OS=Parasphingopyxis lamellibrachiae OX=680125 GN=DFR46_2064 PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 9.82RR3 pKa = 11.84HH4 pKa = 6.17SIINFATVAMSGALLAACGGTAPDD28 pKa = 4.07EE29 pKa = 4.43PVEE32 pKa = 4.29EE33 pKa = 4.32PAVEE37 pKa = 4.14VEE39 pKa = 4.55PLADD43 pKa = 3.63ACPDD47 pKa = 3.13RR48 pKa = 11.84VQDD51 pKa = 3.72MRR53 pKa = 11.84GSTDD57 pKa = 4.7AFTCSCSTEE66 pKa = 3.93TAEE69 pKa = 6.13AGTVWGAGPYY79 pKa = 9.95SDD81 pKa = 5.82DD82 pKa = 4.17SAVCRR87 pKa = 11.84AAMHH91 pKa = 6.61AGLVGDD97 pKa = 4.56EE98 pKa = 4.35PANVTINFMDD108 pKa = 3.89GRR110 pKa = 11.84DD111 pKa = 3.46SYY113 pKa = 10.79TASEE117 pKa = 4.3ANGVEE122 pKa = 4.1TRR124 pKa = 11.84QWGSWGGSFAFEE136 pKa = 4.04GAEE139 pKa = 3.85LGEE142 pKa = 4.29PEE144 pKa = 4.48GEE146 pKa = 3.76EE147 pKa = 3.93TAALEE152 pKa = 4.32ACPGNARR159 pKa = 11.84QLRR162 pKa = 11.84GTEE165 pKa = 3.9EE166 pKa = 4.41AVQCSCTAQATEE178 pKa = 5.28DD179 pKa = 3.73GTVWGSGTYY188 pKa = 9.76TDD190 pKa = 4.75DD191 pKa = 4.77SGVCRR196 pKa = 11.84AALHH200 pKa = 6.11AGIVTGDD207 pKa = 3.47GGDD210 pKa = 3.43VTFTIVDD217 pKa = 3.85GQEE220 pKa = 4.09SYY222 pKa = 11.07SGSEE226 pKa = 3.93ANGVEE231 pKa = 4.03SRR233 pKa = 11.84DD234 pKa = 3.48YY235 pKa = 10.95GAWSGSFEE243 pKa = 4.41FEE245 pKa = 4.5DD246 pKa = 5.01DD247 pKa = 3.58KK248 pKa = 11.99GG249 pKa = 3.61

Molecular weight: 25.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D9FCI2|A0A3D9FCI2_9SPHN Beta-carotene hydroxylase OS=Parasphingopyxis lamellibrachiae OX=680125 GN=DFR46_0280 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.51GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.83ATVGGRR28 pKa = 11.84NVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.7GRR39 pKa = 11.84KK40 pKa = 8.77KK41 pKa = 10.66LSAA44 pKa = 3.95

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2877

0

2877

903915

29

2922

314.2

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.306 ± 0.06

0.832 ± 0.016

6.28 ± 0.035

6.444 ± 0.046

3.739 ± 0.035

8.786 ± 0.053

1.97 ± 0.022

5.633 ± 0.033

2.854 ± 0.045

9.529 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.022

2.779 ± 0.028

5.063 ± 0.031

3.035 ± 0.025

7.029 ± 0.047

5.477 ± 0.033

5.251 ± 0.035

6.739 ± 0.034

1.404 ± 0.021

2.276 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski