Salinisphaera japonica YTM-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Salinisphaerales; Salinisphaeraceae; Salinisphaera; Salinisphaera japonica

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2932 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A423PFV1|A0A423PFV1_9GAMM Uncharacterized protein OS=Salinisphaera japonica YTM-1 OX=1209778 GN=SAJA_14245 PE=4 SV=1
MM1 pKa = 7.6LATPVSCRR9 pKa = 11.84GDD11 pKa = 3.51YY12 pKa = 10.8EE13 pKa = 4.12IVRR16 pKa = 11.84TYY18 pKa = 10.94IDD20 pKa = 4.22LCTSGIFEE28 pKa = 6.14FPFDD32 pKa = 4.67WNDD35 pKa = 3.03LDD37 pKa = 3.42IWNDD41 pKa = 3.48PLEE44 pKa = 4.22MSRR47 pKa = 11.84QGGGFILADD56 pKa = 3.85GVNSEE61 pKa = 4.66YY62 pKa = 10.18VTTDD66 pKa = 2.32ISGFKK71 pKa = 10.18LIWVHH76 pKa = 6.72

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A423PZF1|A0A423PZF1_9GAMM Uncharacterized protein OS=Salinisphaera japonica YTM-1 OX=1209778 GN=SAJA_03850 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.16RR14 pKa = 11.84THH16 pKa = 5.96GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.01NGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LTPP44 pKa = 3.95

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2932

0

2932

946517

29

10462

322.8

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.335 ± 0.067

0.889 ± 0.016

6.695 ± 0.063

5.067 ± 0.052

3.403 ± 0.028

8.377 ± 0.061

2.317 ± 0.034

5.088 ± 0.028

2.631 ± 0.049

9.827 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.337 ± 0.026

2.789 ± 0.045

4.796 ± 0.032

3.48 ± 0.03

7.113 ± 0.056

5.07 ± 0.037

5.614 ± 0.064

7.269 ± 0.041

1.391 ± 0.023

2.512 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski