Streptomyces phage ZL12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0UWB5|D0UWB5_9CAUD Uncharacterized protein OS=Streptomyces phage ZL12 OX=2570911 GN=pZL12.10c PE=4 SV=1
MM1 pKa = 7.44TEE3 pKa = 4.11RR4 pKa = 11.84PHH6 pKa = 6.24EE7 pKa = 4.64HH8 pKa = 6.67GSYY11 pKa = 9.95YY12 pKa = 10.13WNADD16 pKa = 3.43SPHH19 pKa = 6.74CKK21 pKa = 10.02HH22 pKa = 6.3GAEE25 pKa = 4.81PDD27 pKa = 3.32WNTDD31 pKa = 2.73GEE33 pKa = 4.45AWDD36 pKa = 3.79RR37 pKa = 11.84WSDD40 pKa = 3.4KK41 pKa = 11.19HH42 pKa = 6.24PVSDD46 pKa = 5.28DD47 pKa = 3.18GHH49 pKa = 6.7ICLDD53 pKa = 3.62APAGEE58 pKa = 4.55ACLDD62 pKa = 3.8CSTEE66 pKa = 4.07EE67 pKa = 4.3GDD69 pKa = 3.71MVPWADD75 pKa = 3.23CRR77 pKa = 11.84TRR79 pKa = 11.84EE80 pKa = 4.03HH81 pKa = 6.85ARR83 pKa = 11.84PKK85 pKa = 10.47HH86 pKa = 5.81GIVPNPGVEE95 pKa = 4.16HH96 pKa = 5.55QQVAVWIGTRR106 pKa = 11.84EE107 pKa = 4.0CLEE110 pKa = 4.61RR111 pKa = 11.84DD112 pKa = 3.45CEE114 pKa = 4.87DD115 pKa = 3.95YY116 pKa = 9.66FTEE119 pKa = 5.99DD120 pKa = 3.23GDD122 pKa = 4.33EE123 pKa = 4.19IPGKK127 pKa = 9.91DD128 pKa = 2.73RR129 pKa = 11.84CFHH132 pKa = 5.99IRR134 pKa = 11.84EE135 pKa = 4.09EE136 pKa = 4.38QACSCQRR143 pKa = 11.84GEE145 pKa = 4.17DD146 pKa = 3.86GEE148 pKa = 4.54YY149 pKa = 10.82SDD151 pKa = 4.7TLCTATANAAAA162 pKa = 4.61

Molecular weight: 18.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0UWL3|D0UWL3_9CAUD Uncharacterized protein OS=Streptomyces phage ZL12 OX=2570911 GN=pZL12.108c PE=4 SV=1
MM1 pKa = 7.04TSKK4 pKa = 10.8AVRR7 pKa = 11.84ATAAYY12 pKa = 8.75AALNAAHH19 pKa = 6.88EE20 pKa = 4.49VGDD23 pKa = 3.42YY24 pKa = 8.9WVQVDD29 pKa = 4.08RR30 pKa = 11.84DD31 pKa = 3.74AVAKK35 pKa = 10.14GGHH38 pKa = 5.12GAEE41 pKa = 4.36GRR43 pKa = 11.84AACARR48 pKa = 11.84HH49 pKa = 4.98VASYY53 pKa = 8.52TATQAVALAAADD65 pKa = 3.98RR66 pKa = 11.84ALGLRR71 pKa = 11.84LHH73 pKa = 6.82WGWAAAGLAVSALTHH88 pKa = 5.43YY89 pKa = 10.89AADD92 pKa = 3.84RR93 pKa = 11.84SGGRR97 pKa = 11.84WRR99 pKa = 11.84EE100 pKa = 4.1DD101 pKa = 3.54GPSTRR106 pKa = 11.84LVRR109 pKa = 11.84FARR112 pKa = 11.84RR113 pKa = 11.84TGHH116 pKa = 5.78TGWLARR122 pKa = 11.84DD123 pKa = 3.81PGAGPLLDD131 pKa = 4.02QAWHH135 pKa = 6.44KK136 pKa = 10.9GWIAVAAVVAAGGGRR151 pKa = 3.32

Molecular weight: 15.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

112

0

112

25941

56

1518

231.6

24.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.55 ± 0.381

0.971 ± 0.099

6.434 ± 0.19

5.293 ± 0.258

1.958 ± 0.114

8.562 ± 0.257

2.51 ± 0.142

3.435 ± 0.186

2.718 ± 0.237

8.007 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.881 ± 0.082

2.729 ± 0.16

6.977 ± 0.305

3.654 ± 0.124

8.034 ± 0.288

4.984 ± 0.216

7.586 ± 0.332

6.681 ± 0.225

1.789 ± 0.123

2.247 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski