Escherichia phage vB_EcoS_XY2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Kagunavirus; unclassified Kagunavirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XMW9|A0A6G6XMW9_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_XY2 OX=2681675 GN=GT372_00019 PE=4 SV=1
MM1 pKa = 7.79EE2 pKa = 4.91EE3 pKa = 4.58LICIEE8 pKa = 4.23SRR10 pKa = 11.84QDD12 pKa = 3.3VQDD15 pKa = 3.45WFVKK19 pKa = 9.13GNKK22 pKa = 9.11YY23 pKa = 10.65SSGVYY28 pKa = 9.16YY29 pKa = 10.58DD30 pKa = 3.09GRR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 10.58VLDD37 pKa = 3.79EE38 pKa = 6.67DD39 pKa = 4.16GDD41 pKa = 3.84EE42 pKa = 4.32WDD44 pKa = 5.0LEE46 pKa = 4.38DD47 pKa = 4.72TVGEE51 pKa = 4.17TLTVSFLQCCFRR63 pKa = 11.84IIHH66 pKa = 6.27NGAEE70 pKa = 4.23SS71 pKa = 3.04

Molecular weight: 8.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XM64|A0A6G6XM64_9CAUD Transcriptional repressor OS=Escherichia phage vB_EcoS_XY2 OX=2681675 GN=GT372_00015 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84EE3 pKa = 3.91AFEE6 pKa = 3.82RR7 pKa = 11.84WAVVEE12 pKa = 4.09GLPVSKK18 pKa = 10.43GLKK21 pKa = 9.69KK22 pKa = 10.49EE23 pKa = 3.87YY24 pKa = 10.76LNVKK28 pKa = 8.31TRR30 pKa = 11.84LAWRR34 pKa = 11.84AWKK37 pKa = 10.47AGALFIANRR46 pKa = 11.84RR47 pKa = 3.7

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13690

34

831

187.5

20.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.073 ± 0.498

1.147 ± 0.126

6.063 ± 0.238

6.866 ± 0.454

3.776 ± 0.175

7.582 ± 0.234

1.702 ± 0.178

5.405 ± 0.164

5.683 ± 0.398

7.487 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.163

4.164 ± 0.248

3.806 ± 0.214

3.842 ± 0.326

5.442 ± 0.241

5.902 ± 0.36

6.603 ± 0.438

7.151 ± 0.326

1.417 ± 0.134

3.419 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski