Camponotus floridanus (Florida carpenter ant)
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14787 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2AWA8|E2AWA8_CAMFO Tryptophan 2 3-dioxygenase OS=Camponotus floridanus OX=104421 GN=EAG_03979 PE=3 SV=1
MM1 pKa = 6.66 TQYY4 pKa = 11.21 SFDD7 pKa = 3.57 MSLTLTLTGGSSVLAVSYY25 pKa = 9.91 FPAIDD30 pKa = 3.95 LTDD33 pKa = 3.55 ADD35 pKa = 4.51 YY36 pKa = 11.53 EE37 pKa = 4.31 LGLTDD42 pKa = 4.37 FEE44 pKa = 5.13 TYY46 pKa = 8.55 HH47 pKa = 6.8 TIPNVNFSNNKK58 pKa = 9.59 FYY60 pKa = 10.93 FGNDD64 pKa = 3.31 DD65 pKa = 3.85 KK66 pKa = 11.48 EE67 pKa = 4.05 ITIPEE72 pKa = 4.18 GSYY75 pKa = 10.27 EE76 pKa = 3.72 LHH78 pKa = 7.45 AINDD82 pKa = 3.68 YY83 pKa = 10.74 LKK85 pKa = 10.68 RR86 pKa = 11.84 AILRR90 pKa = 11.84 DD91 pKa = 3.61 GTPARR96 pKa = 11.84 DD97 pKa = 3.29 VEE99 pKa = 3.99 NDD101 pKa = 3.04 YY102 pKa = 11.65 DD103 pKa = 4.37 EE104 pKa = 5.79 EE105 pKa = 4.49 YY106 pKa = 10.97 QQ107 pKa = 4.05
Molecular weight: 12.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.791
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.694
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.834
Nozaki 4.012
DTASelect 4.228
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.024
Patrickios 0.655
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|E2A8F8|E2A8F8_CAMFO RNA 3'-terminal phosphate cyclase OS=Camponotus floridanus OX=104421 GN=EAG_06631 PE=3 SV=1
YYY2 pKa = 8.45 STRR5 pKa = 11.84 THHH8 pKa = 6.27 RR9 pKa = 11.84 THHH12 pKa = 5.85 RR13 pKa = 11.84 THHH16 pKa = 5.84 RR17 pKa = 11.84 THHH20 pKa = 5.84 RR21 pKa = 11.84 THHH24 pKa = 5.84 RR25 pKa = 11.84 THHH28 pKa = 5.84 RR29 pKa = 11.84 THHH32 pKa = 5.84 RR33 pKa = 11.84 THHH36 pKa = 5.84 RR37 pKa = 11.84 THHH40 pKa = 5.84 RR41 pKa = 11.84 THHH44 pKa = 5.84 RR45 pKa = 11.84 THHH48 pKa = 5.84 RR49 pKa = 11.84 THHH52 pKa = 5.84 RR53 pKa = 11.84 THHH56 pKa = 5.84 RR57 pKa = 11.84 THHH60 pKa = 5.84 RR61 pKa = 11.84 THHH64 pKa = 5.84 RR65 pKa = 11.84 THHH68 pKa = 5.84 RR69 pKa = 11.84 THHH72 pKa = 5.84 RR73 pKa = 11.84 THHH76 pKa = 5.84 RR77 pKa = 11.84 THHH80 pKa = 5.84 RR81 pKa = 11.84 THHH84 pKa = 5.84 RR85 pKa = 11.84 THHH88 pKa = 5.84 RR89 pKa = 11.84 THHH92 pKa = 5.84 RR93 pKa = 11.84 THHH96 pKa = 5.84 RR97 pKa = 11.84 THHH100 pKa = 5.84 RR101 pKa = 11.84 THHH104 pKa = 5.84 RR105 pKa = 11.84 THHH108 pKa = 5.84 RR109 pKa = 11.84 THHH112 pKa = 5.84 RR113 pKa = 11.84 THHH116 pKa = 5.84 RR117 pKa = 11.84 THHH120 pKa = 5.92 RR121 pKa = 11.84 THHH124 pKa = 4.62 TVFSFNLVFKKK135 pKa = 10.91 YY136 pKa = 9.26 TICINFVHHH145 pKa = 6.89 L
Molecular weight: 17.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.478
IPC2_protein 11.228
IPC_protein 12.661
Toseland 12.808
ProMoST 13.32
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.325
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.32
Sillero 12.808
Patrickios 12.047
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14787
0
14787
6383122
9
12221
431.7
48.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.12 ± 0.019
1.998 ± 0.031
5.43 ± 0.016
6.722 ± 0.031
3.6 ± 0.016
5.266 ± 0.029
2.523 ± 0.011
6.22 ± 0.02
6.407 ± 0.029
9.042 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.306 ± 0.01
5.218 ± 0.022
4.921 ± 0.029
4.192 ± 0.02
5.801 ± 0.023
8.105 ± 0.029
5.985 ± 0.021
5.832 ± 0.014
1.102 ± 0.008
3.209 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here