Amino acid dipepetide frequency for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker s yeast)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.973AlaAla: 3.973 ± 0.055	0.733AlaCys: 0.733 ± 0.018	2.644AlaAsp: 2.644 ± 0.033	3.055AlaGlu: 3.055 ± 0.038	2.276AlaPhe: 2.276 ± 0.031	2.812AlaGly: 2.812 ± 0.039	1.151AlaHis: 1.151 ± 0.022	3.676AlaIle: 3.676 ± 0.042	3.878AlaLys: 3.878 ± 0.043	5.252AlaLeu: 5.252 ± 0.053	1.264AlaMet: 1.264 ± 0.023	3.157AlaAsn: 3.157 ± 0.036	2.365AlaPro: 2.365 ± 0.037	2.115AlaGln: 2.115 ± 0.037	2.425AlaArg: 2.425 ± 0.034	5.143AlaSer: 5.143 ± 0.054	3.598AlaThr: 3.598 ± 0.052	3.186AlaVal: 3.186 ± 0.041	0.505AlaTrp: 0.505 ± 0.015	1.722AlaTyr: 1.722 ± 0.028	0.0AlaXaa: 0.0 ± 0.0
Cys	0.656CysAla: 0.656 ± 0.018	0.296CysCys: 0.296 ± 0.011	0.657CysAsp: 0.657 ± 0.018	0.614CysGlu: 0.614 ± 0.015	0.682CysPhe: 0.682 ± 0.017	0.824CysGly: 0.824 ± 0.019	0.333CysHis: 0.333 ± 0.012	0.936CysIle: 0.936 ± 0.019	0.755CysLys: 0.755 ± 0.016	1.327CysLeu: 1.327 ± 0.025	0.279CysMet: 0.279 ± 0.009	0.598CysAsn: 0.598 ± 0.016	0.562CysPro: 0.562 ± 0.016	0.499CysGln: 0.499 ± 0.014	0.523CysArg: 0.523 ± 0.013	1.105CysSer: 1.105 ± 0.02	0.66CysThr: 0.66 ± 0.017	0.751CysVal: 0.751 ± 0.016	0.152CysTrp: 0.152 ± 0.007	0.476CysTyr: 0.476 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	3.118AspAla: 3.118 ± 0.032	0.668AspCys: 0.668 ± 0.016	4.64AspAsp: 4.64 ± 0.065	5.012AspGlu: 5.012 ± 0.057	2.704AspPhe: 2.704 ± 0.032	2.85AspGly: 2.85 ± 0.037	1.105AspHis: 1.105 ± 0.019	4.366AspIle: 4.366 ± 0.039	3.52AspLys: 3.52 ± 0.041	5.581AspLeu: 5.581 ± 0.047	1.228AspMet: 1.228 ± 0.019	3.23AspAsn: 3.23 ± 0.04	2.451AspPro: 2.451 ± 0.028	1.722AspGln: 1.722 ± 0.027	2.107AspArg: 2.107 ± 0.029	4.912AspSer: 4.912 ± 0.052	3.022AspThr: 3.022 ± 0.039	3.418AspVal: 3.418 ± 0.036	0.632AspTrp: 0.632 ± 0.018	2.088AspTyr: 2.088 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	3.426GluAla: 3.426 ± 0.042	0.659GluCys: 0.659 ± 0.016	4.324GluAsp: 4.324 ± 0.052	5.928GluGlu: 5.928 ± 0.082	2.684GluPhe: 2.684 ± 0.035	2.704GluGly: 2.704 ± 0.032	1.219GluHis: 1.219 ± 0.021	4.317GluIle: 4.317 ± 0.04	5.413GluLys: 5.413 ± 0.058	6.294GluLeu: 6.294 ± 0.055	1.379GluMet: 1.379 ± 0.023	4.454GluAsn: 4.454 ± 0.046	2.078GluPro: 2.078 ± 0.026	2.564GluGln: 2.564 ± 0.034	2.97GluArg: 2.97 ± 0.037	4.911GluSer: 4.911 ± 0.053	3.655GluThr: 3.655 ± 0.036	3.4GluVal: 3.4 ± 0.035	0.628GluTrp: 0.628 ± 0.016	2.228GluTyr: 2.228 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	2.367PheAla: 2.367 ± 0.036	0.576PheCys: 0.576 ± 0.015	2.672PheAsp: 2.672 ± 0.036	2.766PheGlu: 2.766 ± 0.034	2.173PhePhe: 2.173 ± 0.039	2.496PheGly: 2.496 ± 0.047	1.056PheHis: 1.056 ± 0.022	3.035PheIle: 3.035 ± 0.038	3.01PheLys: 3.01 ± 0.036	4.288PheLeu: 4.288 ± 0.044	0.906PheMet: 0.906 ± 0.018	2.61PheAsn: 2.61 ± 0.033	1.903PhePro: 1.903 ± 0.026	1.992PheGln: 1.992 ± 0.026	1.697PheArg: 1.697 ± 0.028	3.606PheSer: 3.606 ± 0.039	2.543PheThr: 2.543 ± 0.036	2.489PheVal: 2.489 ± 0.038	0.516PheTrp: 0.516 ± 0.015	1.641PheTyr: 1.641 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	2.76GlyAla: 2.76 ± 0.041	0.741GlyCys: 0.741 ± 0.018	2.696GlyAsp: 2.696 ± 0.032	2.853GlyGlu: 2.853 ± 0.033	2.273GlyPhe: 2.273 ± 0.032	3.115GlyGly: 3.115 ± 0.046	1.115GlyHis: 1.115 ± 0.021	3.439GlyIle: 3.439 ± 0.041	3.713GlyLys: 3.713 ± 0.036	4.552GlyLeu: 4.552 ± 0.044	1.054GlyMet: 1.054 ± 0.019	2.927GlyAsn: 2.927 ± 0.038	1.683GlyPro: 1.683 ± 0.026	1.663GlyGln: 1.663 ± 0.03	2.084GlyArg: 2.084 ± 0.033	4.628GlySer: 4.628 ± 0.048	3.012GlyThr: 3.012 ± 0.036	2.977GlyVal: 2.977 ± 0.036	0.601GlyTrp: 0.601 ± 0.015	1.821GlyTyr: 1.821 ± 0.028	0.0GlyXaa: 0.0 ± 0.0
His	1.072HisAla: 1.072 ± 0.02	0.339HisCys: 0.339 ± 0.012	1.253HisAsp: 1.253 ± 0.021	1.274HisGlu: 1.274 ± 0.021	0.973HisPhe: 0.973 ± 0.018	1.162HisGly: 1.162 ± 0.019	0.74HisHis: 0.74 ± 0.022	1.43HisIle: 1.43 ± 0.024	1.319HisLys: 1.319 ± 0.023	2.19HisLeu: 2.19 ± 0.029	0.43HisMet: 0.43 ± 0.012	1.205HisAsn: 1.205 ± 0.023	1.138HisPro: 1.138 ± 0.022	0.824HisGln: 0.824 ± 0.017	1.024HisArg: 1.024 ± 0.022	1.964HisSer: 1.964 ± 0.032	1.152HisThr: 1.152 ± 0.024	1.159HisVal: 1.159 ± 0.021	0.256HisTrp: 0.256 ± 0.01	0.813HisTyr: 0.813 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	3.624IleAla: 3.624 ± 0.035	0.935IleCys: 0.935 ± 0.018	3.987IleAsp: 3.987 ± 0.039	4.079IleGlu: 4.079 ± 0.035	3.006IlePhe: 3.006 ± 0.037	3.129IleGly: 3.129 ± 0.041	1.455IleHis: 1.455 ± 0.026	4.345IleIle: 4.345 ± 0.051	4.627IleLys: 4.627 ± 0.041	6.321IleLeu: 6.321 ± 0.059	1.386IleMet: 1.386 ± 0.022	3.945IleAsn: 3.945 ± 0.041	3.294IlePro: 3.294 ± 0.039	2.409IleGln: 2.409 ± 0.032	2.804IleArg: 2.804 ± 0.031	5.914IleSer: 5.914 ± 0.046	3.775IleThr: 3.775 ± 0.04	3.679IleVal: 3.679 ± 0.036	0.747IleTrp: 0.747 ± 0.018	2.11IleTyr: 2.11 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.67LysAla: 3.67 ± 0.038	0.825LysCys: 0.825 ± 0.02	4.167LysAsp: 4.167 ± 0.043	5.37LysGlu: 5.37 ± 0.062	3.291LysPhe: 3.291 ± 0.039	2.986LysGly: 2.986 ± 0.034	1.467LysHis: 1.467 ± 0.021	4.85LysIle: 4.85 ± 0.047	6.781LysLys: 6.781 ± 0.07	7.051LysLeu: 7.051 ± 0.051	1.53LysMet: 1.53 ± 0.024	4.551LysAsn: 4.551 ± 0.04	2.987LysPro: 2.987 ± 0.037	2.76LysGln: 2.76 ± 0.035	3.997LysArg: 3.997 ± 0.039	5.647LysSer: 5.647 ± 0.055	4.016LysThr: 4.016 ± 0.039	3.965LysVal: 3.965 ± 0.041	0.77LysTrp: 0.77 ± 0.016	2.744LysTyr: 2.744 ± 0.031	0.0LysXaa: 0.0 ± 0.0
Leu	5.276LeuAla: 5.276 ± 0.053	1.317LeuCys: 1.317 ± 0.023	5.324LeuAsp: 5.324 ± 0.05	5.997LeuGlu: 5.997 ± 0.062	4.17LeuPhe: 4.17 ± 0.047	4.472LeuGly: 4.472 ± 0.04	2.151LeuHis: 2.151 ± 0.03	5.785LeuIle: 5.785 ± 0.051	7.442LeuLys: 7.442 ± 0.069	9.386LeuLeu: 9.386 ± 0.089	1.927LeuMet: 1.927 ± 0.029	5.782LeuAsn: 5.782 ± 0.055	4.712LeuPro: 4.712 ± 0.043	4.361LeuGln: 4.361 ± 0.047	4.448LeuArg: 4.448 ± 0.045	8.32LeuSer: 8.32 ± 0.053	5.223LeuThr: 5.223 ± 0.046	4.991LeuVal: 4.991 ± 0.05	0.896LeuTrp: 0.896 ± 0.018	3.089LeuTyr: 3.089 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	1.352MetAla: 1.352 ± 0.022	0.244MetCys: 0.244 ± 0.009	1.308MetAsp: 1.308 ± 0.024	1.351MetGlu: 1.351 ± 0.023	0.866MetPhe: 0.866 ± 0.02	1.155MetGly: 1.155 ± 0.023	0.381MetHis: 0.381 ± 0.012	1.312MetIle: 1.312 ± 0.019	1.587MetLys: 1.587 ± 0.026	1.798MetLeu: 1.798 ± 0.028	0.523MetMet: 0.523 ± 0.015	1.344MetAsn: 1.344 ± 0.025	0.789MetPro: 0.789 ± 0.019	0.755MetGln: 0.755 ± 0.017	0.877MetArg: 0.877 ± 0.017	1.9MetSer: 1.9 ± 0.026	1.328MetThr: 1.328 ± 0.021	1.215MetVal: 1.215 ± 0.023	0.187MetTrp: 0.187 ± 0.009	0.588MetTyr: 0.588 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.216AsnAla: 3.216 ± 0.039	0.715AsnCys: 0.715 ± 0.016	4.08AsnAsp: 4.08 ± 0.047	4.28AsnGlu: 4.28 ± 0.046	2.583AsnPhe: 2.583 ± 0.037	3.489AsnGly: 3.489 ± 0.04	1.235AsnHis: 1.235 ± 0.021	4.082AsnIle: 4.082 ± 0.042	4.322AsnLys: 4.322 ± 0.045	5.149AsnLeu: 5.149 ± 0.047	1.258AsnMet: 1.258 ± 0.02	4.878AsnAsn: 4.878 ± 0.091	2.443AsnPro: 2.443 ± 0.033	1.969AsnGln: 1.969 ± 0.027	2.264AsnArg: 2.264 ± 0.028	5.898AsnSer: 5.898 ± 0.073	3.534AsnThr: 3.534 ± 0.042	3.457AsnVal: 3.457 ± 0.039	0.667AsnTrp: 0.667 ± 0.016	2.043AsnTyr: 2.043 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	2.243ProAla: 2.243 ± 0.035	0.386ProCys: 0.386 ± 0.013	2.181ProAsp: 2.181 ± 0.025	2.901ProGlu: 2.901 ± 0.032	1.92ProPhe: 1.92 ± 0.03	1.923ProGly: 1.923 ± 0.028	1.021ProHis: 1.021 ± 0.02	2.897ProIle: 2.897 ± 0.032	2.889ProLys: 2.889 ± 0.032	4.198ProLeu: 4.198 ± 0.037	0.861ProMet: 0.861 ± 0.015	2.514ProAsn: 2.514 ± 0.032	2.507ProPro: 2.507 ± 0.061	1.994ProGln: 1.994 ± 0.038	1.793ProArg: 1.793 ± 0.025	4.321ProSer: 4.321 ± 0.053	2.777ProThr: 2.777 ± 0.04	2.595ProVal: 2.595 ± 0.035	0.444ProTrp: 0.444 ± 0.014	1.441ProTyr: 1.441 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	1.952GlnAla: 1.952 ± 0.036	0.463GlnCys: 0.463 ± 0.013	2.103GlnAsp: 2.103 ± 0.031	2.778GlnGlu: 2.778 ± 0.033	1.764GlnPhe: 1.764 ± 0.027	1.73GlnGly: 1.73 ± 0.027	0.9GlnHis: 0.9 ± 0.021	2.459GlnIle: 2.459 ± 0.026	2.753GlnLys: 2.753 ± 0.03	4.073GlnLeu: 4.073 ± 0.041	0.866GlnMet: 0.866 ± 0.018	2.385GlnAsn: 2.385 ± 0.034	1.617GlnPro: 1.617 ± 0.031	2.876GlnGln: 2.876 ± 0.093	1.999GlnArg: 1.999 ± 0.026	2.911GlnSer: 2.911 ± 0.035	1.99GlnThr: 1.99 ± 0.027	1.993GlnVal: 1.993 ± 0.027	0.417GlnTrp: 0.417 ± 0.012	1.473GlnTyr: 1.473 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	2.282ArgAla: 2.282 ± 0.031	0.588ArgCys: 0.588 ± 0.014	2.443ArgAsp: 2.443 ± 0.033	2.756ArgGlu: 2.756 ± 0.036	1.935ArgPhe: 1.935 ± 0.026	2.161ArgGly: 2.161 ± 0.043	1.045ArgHis: 1.045 ± 0.02	2.889ArgIle: 2.889 ± 0.036	3.698ArgLys: 3.698 ± 0.045	4.239ArgLeu: 4.239 ± 0.044	0.94ArgMet: 0.94 ± 0.02	2.768ArgAsn: 2.768 ± 0.033	1.687ArgPro: 1.687 ± 0.025	1.771ArgGln: 1.771 ± 0.026	2.8ArgArg: 2.8 ± 0.04	3.695ArgSer: 3.695 ± 0.041	2.466ArgThr: 2.466 ± 0.031	2.227ArgVal: 2.227 ± 0.03	0.473ArgTrp: 0.473 ± 0.013	1.576ArgTyr: 1.576 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	4.849SerAla: 4.849 ± 0.053	0.99SerCys: 0.99 ± 0.021	4.666SerAsp: 4.666 ± 0.048	4.682SerGlu: 4.682 ± 0.054	3.778SerPhe: 3.778 ± 0.043	4.351SerGly: 4.351 ± 0.046	1.915SerHis: 1.915 ± 0.027	5.613SerIle: 5.613 ± 0.048	6.559SerLys: 6.559 ± 0.055	8.339SerLeu: 8.339 ± 0.061	1.857SerMet: 1.857 ± 0.026	5.814SerAsn: 5.814 ± 0.071	3.925SerPro: 3.925 ± 0.059	3.548SerGln: 3.548 ± 0.037	3.945SerArg: 3.945 ± 0.041	10.719SerSer: 10.719 ± 0.196	6.26SerThr: 6.26 ± 0.092	4.62SerVal: 4.62 ± 0.039	0.795SerTrp: 0.795 ± 0.017	2.582SerTyr: 2.582 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	3.507ThrAla: 3.507 ± 0.037	0.67ThrCys: 0.67 ± 0.018	3.075ThrAsp: 3.075 ± 0.036	3.341ThrGlu: 3.341 ± 0.042	2.604ThrPhe: 2.604 ± 0.032	3.054ThrGly: 3.054 ± 0.04	1.175ThrHis: 1.175 ± 0.021	3.784ThrIle: 3.784 ± 0.038	4.164ThrLys: 4.164 ± 0.039	5.44ThrLeu: 5.44 ± 0.044	1.075ThrMet: 1.075 ± 0.02	3.708ThrAsn: 3.708 ± 0.054	3.052ThrPro: 3.052 ± 0.04	1.951ThrGln: 1.951 ± 0.025	2.385ThrArg: 2.385 ± 0.027	5.75ThrSer: 5.75 ± 0.087	4.475ThrThr: 4.475 ± 0.091	3.556ThrVal: 3.556 ± 0.039	0.626ThrTrp: 0.626 ± 0.017	1.74ThrTyr: 1.74 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	3.364ValAla: 3.364 ± 0.044	0.77ValCys: 0.77 ± 0.018	3.348ValAsp: 3.348 ± 0.037	3.516ValGlu: 3.516 ± 0.043	2.463ValPhe: 2.463 ± 0.035	2.879ValGly: 2.879 ± 0.042	1.193ValHis: 1.193 ± 0.021	3.547ValIle: 3.547 ± 0.042	3.865ValLys: 3.865 ± 0.033	5.264ValLeu: 5.264 ± 0.05	1.119ValMet: 1.119 ± 0.021	3.059ValAsn: 3.059 ± 0.034	2.882ValPro: 2.882 ± 0.04	1.963ValGln: 1.963 ± 0.027	2.359ValArg: 2.359 ± 0.033	4.889ValSer: 4.889 ± 0.047	3.294ValThr: 3.294 ± 0.039	3.411ValVal: 3.411 ± 0.043	0.552ValTrp: 0.552 ± 0.015	1.844ValTyr: 1.844 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	0.518TrpAla: 0.518 ± 0.014	0.23TrpCys: 0.23 ± 0.009	0.658TrpAsp: 0.658 ± 0.016	0.638TrpGlu: 0.638 ± 0.016	0.519TrpPhe: 0.519 ± 0.015	0.507TrpGly: 0.507 ± 0.015	0.22TrpHis: 0.22 ± 0.009	0.639TrpIle: 0.639 ± 0.015	0.875TrpLys: 0.875 ± 0.022	0.985TrpLeu: 0.985 ± 0.018	0.226TrpMet: 0.226 ± 0.008	0.666TrpAsn: 0.666 ± 0.014	0.301TrpPro: 0.301 ± 0.01	0.38TrpGln: 0.38 ± 0.01	0.503TrpArg: 0.503 ± 0.014	0.886TrpSer: 0.886 ± 0.019	0.512TrpThr: 0.512 ± 0.013	0.615TrpVal: 0.615 ± 0.016	0.147TrpTrp: 0.147 ± 0.008	0.382TrpTyr: 0.382 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.704TyrAla: 1.704 ± 0.025	0.543TyrCys: 0.543 ± 0.015	2.147TyrAsp: 2.147 ± 0.029	2.042TyrGlu: 2.042 ± 0.029	1.68TyrPhe: 1.68 ± 0.032	1.909TyrGly: 1.909 ± 0.029	0.838TyrHis: 0.838 ± 0.016	2.168TyrIle: 2.168 ± 0.028	2.253TyrLys: 2.253 ± 0.029	3.342TyrLeu: 3.342 ± 0.038	0.753TyrMet: 0.753 ± 0.015	1.966TyrAsn: 1.966 ± 0.028	1.43TyrPro: 1.43 ± 0.025	1.384TyrGln: 1.384 ± 0.024	1.437TyrArg: 1.437 ± 0.02	2.689TyrSer: 2.689 ± 0.036	1.841TyrThr: 1.841 ± 0.025	1.877TyrVal: 1.877 ± 0.026	0.398TyrTrp: 0.398 ± 0.013	1.452TyrTyr: 1.452 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6079 proteins (2949596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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