Ricinus communis (Castor bean)
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31219 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B9RZS2|B9RZS2_RICCO UBC core domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_1000700 PE=4 SV=1
MM1 pKa = 7.58 GGGGGNGMDD10 pKa = 4.06 PFEE13 pKa = 6.36 DD14 pKa = 3.22 ITGGGGGGGAALAIPDD30 pKa = 3.97 VLSGGGGGSGSAAVTAPDD48 pKa = 3.79 VIGGGGGGGGNANGTPTAAAAACADD73 pKa = 3.82 ILLSCDD79 pKa = 2.74 IGGDD83 pKa = 3.48 GGGGGGGCCGDD94 pKa = 3.31 GSGNGIQAATLLGCNISGGGGGGGEE119 pKa = 4.31 GGRR122 pKa = 11.84 VGGAATFDD130 pKa = 3.9 CSIGGGGGSDD140 pKa = 3.34 RR141 pKa = 11.84 GNGATTSLACNVGGNGGGVGGGAGMKK167 pKa = 10.29 FGDD170 pKa = 4.56 KK171 pKa = 10.22 IGGGIDD177 pKa = 3.14 SSLISVVAISADD189 pKa = 3.29 PFFILRR195 pKa = 11.84 TVEE198 pKa = 4.03 SS199 pKa = 3.78
Molecular weight: 17.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.643
IPC_protein 3.63
Toseland 3.401
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.656
Rodwell 3.465
Grimsley 3.312
Solomon 3.63
Lehninger 3.592
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.656
Sillero 3.757
Patrickios 0.769
IPC_peptide 3.617
IPC2_peptide 3.719
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|B9TNJ5|B9TNJ5_RICCO Metallophos domain-containing protein OS=Ricinus communis OX=3988 GN=RCOM_2015560 PE=4 SV=1
MM1 pKa = 7.66 KK2 pKa = 9.78 PPSAPSPARR11 pKa = 11.84 AARR14 pKa = 11.84 PPTRR18 pKa = 11.84 KK19 pKa = 9.53 ARR21 pKa = 11.84 RR22 pKa = 11.84 TNSILKK28 pKa = 9.95 RR29 pKa = 11.84 RR30 pKa = 11.84 AGPARR35 pKa = 11.84 KK36 pKa = 8.91 AARR39 pKa = 11.84 RR40 pKa = 11.84 SAATANTWPPSAARR54 pKa = 11.84 AVKK57 pKa = 10.01 AASPPPAPTRR67 pKa = 11.84 KK68 pKa = 9.58 KK69 pKa = 10.14 SAAPGPSNRR78 pKa = 11.84 PRR80 pKa = 11.84 AATSNKK86 pKa = 8.96 RR87 pKa = 11.84 SPRR90 pKa = 11.84 ATPPGARR97 pKa = 11.84 AGIAAWRR104 pKa = 11.84 RR105 pKa = 11.84 GRR107 pKa = 11.84 PAARR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 CWARR117 pKa = 11.84 SGGMAGRR124 pKa = 11.84 RR125 pKa = 11.84 PGG127 pKa = 3.17
Molecular weight: 13.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.413
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.24
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
31219
0
31219
10431927
29
5282
334.2
37.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.415 ± 0.018
1.797 ± 0.008
5.276 ± 0.011
6.235 ± 0.015
4.123 ± 0.009
6.698 ± 0.015
2.427 ± 0.008
5.41 ± 0.01
5.804 ± 0.013
9.739 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.429 ± 0.005
4.381 ± 0.009
4.846 ± 0.014
3.834 ± 0.011
5.584 ± 0.014
8.594 ± 0.018
4.862 ± 0.009
6.484 ± 0.012
1.297 ± 0.005
2.759 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here