Marine Group I thaumarchaeote SCGC AAA799-D07
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 562 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A087S4J2|A0A087S4J2_9ARCH ATP phosphoribosyltransferase OS=Marine Group I thaumarchaeote SCGC AAA799-D07 OX=1502290 GN=hisG PE=4 SV=1
MM1 pKa = 7.23 NNEE4 pKa = 4.48 LGRR7 pKa = 11.84 KK8 pKa = 7.25 ITSLTLMTIMFAGGITVAGSSFAFAPYY35 pKa = 9.34 MPEE38 pKa = 3.91 AMADD42 pKa = 3.56 HH43 pKa = 6.89 SNDD46 pKa = 3.39 AASSGTLSVSSTHH59 pKa = 5.84 VMGGAVLGITVNDD72 pKa = 4.13 PSIGATDD79 pKa = 3.58 QQIAPPSVEE88 pKa = 3.82 YY89 pKa = 10.7 DD90 pKa = 3.55 GQSVDD95 pKa = 3.58 MTQMTDD101 pKa = 3.67 GTWQAYY107 pKa = 8.97 IVDD110 pKa = 4.07 HH111 pKa = 5.74 EE112 pKa = 4.91 TVVGLDD118 pKa = 2.95 GDD120 pKa = 4.15 MEE122 pKa = 4.69 NDD124 pKa = 3.26 NDD126 pKa = 4.35 SGFQYY131 pKa = 10.85 GVYY134 pKa = 9.96 CDD136 pKa = 4.35 SGLGAQGVSAGTGNYY151 pKa = 10.09 DD152 pKa = 3.29 SAGSLDD158 pKa = 4.16 SDD160 pKa = 3.36 ITTNPSFYY168 pKa = 10.4 EE169 pKa = 4.54 GWHH172 pKa = 5.94 GGGAKK177 pKa = 10.27 GKK179 pKa = 10.59 GGGFGQTQYY188 pKa = 11.41 CQDD191 pKa = 3.55 TVDD194 pKa = 4.0 AGDD197 pKa = 4.58 GIGGGQVKK205 pKa = 9.79 FSVLLDD211 pKa = 3.76 APTLNTNSGTGTNNSGNRR229 pKa = 11.84 NIMVNATTNLSAWPFIQAINFSSTADD255 pKa = 3.07 VDD257 pKa = 4.02 YY258 pKa = 10.71 GAEE261 pKa = 4.15 SVTVEE266 pKa = 4.09 WGNDD270 pKa = 2.91 HH271 pKa = 6.87 DD272 pKa = 4.38 TSISVDD278 pKa = 3.3 RR279 pKa = 11.84 DD280 pKa = 3.36 IVPDD284 pKa = 3.45 NAEE287 pKa = 3.56 IHH289 pKa = 5.7 LTIVDD294 pKa = 3.91 PGLNYY299 pKa = 10.85 DD300 pKa = 4.02 PTNEE304 pKa = 4.63 DD305 pKa = 2.86 VWIMDD310 pKa = 4.12 AAQEE314 pKa = 3.97 TLYY317 pKa = 10.61 FYY319 pKa = 11.42 NNGSIGEE326 pKa = 4.34 KK327 pKa = 9.17 NTAQLPTQGASSALTGGEE345 pKa = 3.63 ISIDD349 pKa = 3.4 GTNNSVIDD357 pKa = 3.72 ATTLGNLGCGDD368 pKa = 3.86 NCLLSVGGSPKK379 pKa = 10.57 NVISTNSSKK388 pKa = 11.07 SHH390 pKa = 6.47 FGFANITLTEE400 pKa = 4.24 TGDD403 pKa = 3.41 NTGVFTTFDD412 pKa = 3.5 GQTGVSSLKK421 pKa = 10.23 SATDD425 pKa = 3.42 AGVDD429 pKa = 3.53 SQMTFSYY436 pKa = 10.88 NGDD439 pKa = 3.46 SVTLVIGYY447 pKa = 9.6 NDD449 pKa = 3.43 ASATFTAGDD458 pKa = 2.93 AWLPVEE464 pKa = 4.37 DD465 pKa = 4.32 AVYY468 pKa = 9.78 TITDD472 pKa = 3.38 PDD474 pKa = 3.75 MNKK477 pKa = 10.09 NSLDD481 pKa = 4.82 DD482 pKa = 3.72 EE483 pKa = 4.54 TLDD486 pKa = 4.63 IGDD489 pKa = 3.7 PHH491 pKa = 8.15 DD492 pKa = 5.62 RR493 pKa = 11.84 IPTIITGSPIHH504 pKa = 6.59 VSDD507 pKa = 4.3 RR508 pKa = 11.84 VSTTARR514 pKa = 11.84 EE515 pKa = 4.08 HH516 pKa = 7.17 SVGQQSLAHH525 pKa = 6.15 SSVALSTGSSAANDD539 pKa = 3.33 VFYY542 pKa = 8.51 TTFASNTTDD551 pKa = 2.64 NSKK554 pKa = 10.47 RR555 pKa = 11.84 IKK557 pKa = 10.69 VVVNGIGGGAPTDD570 pKa = 3.76 AEE572 pKa = 4.77 LNTITWLNVTTDD584 pKa = 3.54 IAPSEE589 pKa = 4.04 LVDD592 pKa = 4.88 LEE594 pKa = 4.48 GTVALQYY601 pKa = 10.37 DD602 pKa = 3.67 ISGIAGALGSTDD614 pKa = 2.34 IDD616 pKa = 3.67 AYY618 pKa = 11.26 VMHH621 pKa = 6.6 EE622 pKa = 4.51 TVGSTGSEE630 pKa = 3.85 PGKK633 pKa = 9.48 TMNGTAAIATNVISIVSSGNSASGIFDD660 pKa = 5.14 LDD662 pKa = 3.65 DD663 pKa = 3.7 TTGDD667 pKa = 3.48 TAGDD671 pKa = 3.71 ATIDD675 pKa = 3.39 SAATQKK681 pKa = 10.98 VGVNGVEE688 pKa = 4.4 DD689 pKa = 3.35 AWGVANTNNAVAIVFQITHH708 pKa = 6.74 PAGNTLAANGTCAPGGAGHH727 pKa = 7.6 DD728 pKa = 3.42 KK729 pKa = 11.4 GCFGEE734 pKa = 4.28 YY735 pKa = 10.14 AIALDD740 pKa = 4.27 FVNWDD745 pKa = 3.35 QDD747 pKa = 3.6 NSSKK751 pKa = 10.47 SQNGIYY757 pKa = 10.03 RR758 pKa = 11.84 LEE760 pKa = 4.81 AGEE763 pKa = 4.43 TGDD766 pKa = 3.4 NTGVFTGTVAYY777 pKa = 10.16 AILNNSTMITASGDD791 pKa = 3.46 PGGSSGFTRR800 pKa = 11.84 GGHH803 pKa = 5.78 SGNAEE808 pKa = 4.22 PEE810 pKa = 4.23 PGSAGGVPAPSPSFNGDD827 pKa = 3.16 EE828 pKa = 4.4 LLVLLGKK835 pKa = 10.33 GQTGIDD841 pKa = 3.51 APRR844 pKa = 11.84 VNINDD849 pKa = 3.54 SDD851 pKa = 4.43 TVGGTGTTLGVQLDD865 pKa = 4.4 ANDD868 pKa = 3.72 HH869 pKa = 5.73 TGVAEE874 pKa = 4.53 FDD876 pKa = 3.73 LSSYY880 pKa = 10.64 GADD883 pKa = 3.29 DD884 pKa = 3.61 TATITITDD892 pKa = 4.21 LDD894 pKa = 4.5 LNQDD898 pKa = 3.33 NEE900 pKa = 4.02 QRR902 pKa = 11.84 EE903 pKa = 4.74 VYY905 pKa = 9.09 TNSSGTFQIMIDD917 pKa = 4.29 DD918 pKa = 5.54 DD919 pKa = 5.1 DD920 pKa = 4.5 SQLLGDD926 pKa = 3.66 QVIIEE931 pKa = 4.3 TSSNSGVFSGTFVVPNKK948 pKa = 9.97 LGKK951 pKa = 10.25 DD952 pKa = 3.54 MEE954 pKa = 4.35 LQYY957 pKa = 11.1 YY958 pKa = 10.7 DD959 pKa = 4.94 SVDD962 pKa = 3.38 QSGEE966 pKa = 3.79 DD967 pKa = 3.24 STTYY971 pKa = 10.77 DD972 pKa = 3.3 VASIASNLGTVALDD986 pKa = 3.29 RR987 pKa = 11.84 TSYY990 pKa = 9.56 PVPYY994 pKa = 10.32 LADD997 pKa = 3.48 QLDD1000 pKa = 3.95 EE1001 pKa = 4.63 GDD1003 pKa = 3.76 NTTMTVAKK1011 pKa = 9.49 GNVVGTITVTDD1022 pKa = 4.19 PDD1024 pKa = 3.56 ATADD1028 pKa = 3.62 TFTTSTNANSCEE1040 pKa = 3.86 AGKK1043 pKa = 10.99 VKK1045 pKa = 10.73 VKK1047 pKa = 10.77 LSGTFVYY1054 pKa = 9.43 TAAGSCATAATSTAAQEE1071 pKa = 4.29 LGALTEE1077 pKa = 4.31 TAQGSGSYY1085 pKa = 10.26 EE1086 pKa = 3.63 IDD1088 pKa = 3.42 FTIGGAACGGSNSGTCQTAQTGVNAVPVNSTSILQVEE1125 pKa = 4.95 YY1126 pKa = 10.58 IDD1128 pKa = 4.1 TADD1131 pKa = 3.53 SAGATTTVYY1140 pKa = 11.0 DD1141 pKa = 3.52 SAIFEE1146 pKa = 4.55 LHH1148 pKa = 5.9 TATLSVDD1155 pKa = 2.85 KK1156 pKa = 10.68 DD1157 pKa = 3.99 VYY1159 pKa = 11.03 ILGQDD1164 pKa = 3.48 AVITLSDD1171 pKa = 4.09 LDD1173 pKa = 4.63 LNLDD1177 pKa = 3.53 ADD1179 pKa = 4.58 EE1180 pKa = 4.77 IEE1182 pKa = 4.48 QYY1184 pKa = 10.73 DD1185 pKa = 3.9 LRR1187 pKa = 11.84 LIEE1190 pKa = 4.41 WDD1192 pKa = 3.32 SDD1194 pKa = 3.87 NNSSVLLHH1202 pKa = 6.71 SSHH1205 pKa = 6.28 CTSGCTLDD1213 pKa = 4.05 PSNLTEE1219 pKa = 4.07 TGKK1222 pKa = 8.7 NTGVFQTVFTIPTSMSDD1239 pKa = 2.95 TGAAATSSEE1248 pKa = 4.1 MGDD1251 pKa = 3.63 EE1252 pKa = 4.5 VILTYY1257 pKa = 10.15 RR1258 pKa = 11.84 DD1259 pKa = 3.32 RR1260 pKa = 11.84 GLSGEE1265 pKa = 4.11 KK1266 pKa = 10.25 SYY1268 pKa = 11.87 GDD1270 pKa = 4.15 DD1271 pKa = 3.77 EE1272 pKa = 6.47 LDD1274 pKa = 3.57 VEE1276 pKa = 4.71 ATFSISKK1283 pKa = 10.15 FGAIVEE1289 pKa = 4.18 LDD1291 pKa = 3.23 KK1292 pKa = 11.37 AVYY1295 pKa = 9.99 DD1296 pKa = 3.77 WTDD1299 pKa = 2.9 TVYY1302 pKa = 9.41 ITVTAPDD1309 pKa = 3.55 WNQNSNSEE1317 pKa = 4.21 EE1318 pKa = 4.34 TIGTSALPINANTRR1332 pKa = 11.84 NGKK1335 pKa = 8.98 LCSGTNTYY1343 pKa = 8.44 TLNEE1347 pKa = 4.05 TDD1349 pKa = 3.59 EE1350 pKa = 4.38 NTGVFDD1356 pKa = 5.34 GEE1358 pKa = 3.95 IALTGFAHH1366 pKa = 6.74 TMATSQSYY1374 pKa = 8.36 TPVADD1379 pKa = 3.57 ACSGNTDD1386 pKa = 3.77 GKK1388 pKa = 10.79 LQTAGNMDD1396 pKa = 5.51 GITVSYY1402 pKa = 10.04 EE1403 pKa = 3.62 WVDD1406 pKa = 3.29 NSIAIASAIIQWNIGEE1422 pKa = 4.19 VEE1424 pKa = 4.19 IVDD1427 pKa = 4.29 SAVSPNGSTVIRR1439 pKa = 11.84 VTDD1442 pKa = 3.37 VDD1444 pKa = 3.83 EE1445 pKa = 6.37 DD1446 pKa = 3.74 IDD1448 pKa = 4.07 STIVDD1453 pKa = 3.85 TFKK1456 pKa = 11.23 VDD1458 pKa = 3.33 VFSDD1462 pKa = 3.51 SDD1464 pKa = 3.64 SGGFQATVSEE1474 pKa = 4.48 TGEE1477 pKa = 4.08 GTGVFEE1483 pKa = 5.21 AIIYY1487 pKa = 7.56 FTDD1490 pKa = 3.35 EE1491 pKa = 4.13 TATSGLTLRR1500 pKa = 11.84 VAEE1503 pKa = 4.5 GDD1505 pKa = 4.01 TVTVEE1510 pKa = 4.23 YY1511 pKa = 10.77 TDD1513 pKa = 3.46 VTLPGPDD1520 pKa = 3.54 YY1521 pKa = 8.25 TTADD1525 pKa = 5.12 DD1526 pKa = 3.51 ITIAATTTVGTTTPPLEE1543 pKa = 4.3 RR1544 pKa = 11.84 APAANARR1551 pKa = 11.84 VVDD1554 pKa = 3.99 AFGSSVAEE1562 pKa = 3.88 VSVDD1566 pKa = 3.47 QQVQIAADD1574 pKa = 3.51 VANAQSKK1581 pKa = 7.96 EE1582 pKa = 3.68 QSFAYY1587 pKa = 9.96 LVQVQDD1593 pKa = 3.86 ANGVTVSLAWITGSLNAGQSMSPALSWTPSASGSYY1628 pKa = 8.8 TATVFVWEE1636 pKa = 4.51 SVDD1639 pKa = 3.6 NPVALSPTVTVGIDD1653 pKa = 3.28 VVV1655 pKa = 3.56
Molecular weight: 171.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.617
IPC_protein 3.681
Toseland 3.439
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.63
Sillero 3.808
Patrickios 0.947
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A087S4D8|A0A087S4D8_9ARCH Uncharacterized protein OS=Marine Group I thaumarchaeote SCGC AAA799-D07 OX=1502290 GN=AAA799D07_00253 PE=4 SV=1
MM1 pKa = 7.59 CGKK4 pKa = 10.63 LMATSKK10 pKa = 10.69 AKK12 pKa = 10.46 RR13 pKa = 11.84 SAAAKK18 pKa = 9.55 KK19 pKa = 9.82 AARR22 pKa = 11.84 TRR24 pKa = 11.84 KK25 pKa = 9.66 RR26 pKa = 11.84 NAAKK30 pKa = 10.3 RR31 pKa = 11.84 KK32 pKa = 9.03 AAAKK36 pKa = 9.77 KK37 pKa = 9.77 AAAKK41 pKa = 9.87 RR42 pKa = 11.84 KK43 pKa = 9.32 RR44 pKa = 11.84 RR45 pKa = 11.84 AAAKK49 pKa = 9.94 KK50 pKa = 9.75 GGRR53 pKa = 11.84 KK54 pKa = 9.42 RR55 pKa = 11.84 GGAKK59 pKa = 10.09 KK60 pKa = 10.42 KK61 pKa = 10.39 GGKK64 pKa = 8.45 KK65 pKa = 9.61 RR66 pKa = 11.84 KK67 pKa = 8.19 AAKK70 pKa = 9.78 RR71 pKa = 11.84 KK72 pKa = 6.67 PAKK75 pKa = 9.55 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 3.56
Molecular weight: 8.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.492
IPC2_protein 11.155
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.778
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.486
IPC_peptide 13.364
IPC2_peptide 12.34
IPC2.peptide.svr19 9.064
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
562
0
562
133761
20
1655
238.0
26.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.02 ± 0.111
1.097 ± 0.041
5.709 ± 0.09
6.503 ± 0.106
4.395 ± 0.08
6.54 ± 0.105
1.844 ± 0.044
9.169 ± 0.107
8.997 ± 0.159
8.745 ± 0.123
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.557 ± 0.048
5.367 ± 0.098
3.643 ± 0.071
2.885 ± 0.058
3.52 ± 0.076
7.026 ± 0.092
5.564 ± 0.104
6.393 ± 0.084
0.896 ± 0.04
3.127 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here