Squirrelpox virus
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 141 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1HTQ8|Q1HTQ8_9POXV C5R OS=Squirrelpox virus OX=240426 GN=C5R PE=4 SV=1
MM1 pKa = 7.62 GFLCALFRR9 pKa = 11.84 RR10 pKa = 11.84 CLRR13 pKa = 11.84 PSVPQEE19 pKa = 3.86 SRR21 pKa = 11.84 GDD23 pKa = 3.8 PIVVVKK29 pKa = 10.43 AAAAPLMRR37 pKa = 11.84 PASEE41 pKa = 4.02 EE42 pKa = 4.07 DD43 pKa = 3.48 EE44 pKa = 4.6 SVLEE48 pKa = 4.3 IAQSPASEE56 pKa = 4.14 HH57 pKa = 5.72 TFVISMVEE65 pKa = 3.88 SRR67 pKa = 11.84 GVMSDD72 pKa = 3.6 TEE74 pKa = 4.4 LEE76 pKa = 4.26 SVDD79 pKa = 5.05 LNEE82 pKa = 5.11 CGEE85 pKa = 4.13 DD86 pKa = 3.49 RR87 pKa = 11.84 YY88 pKa = 11.48 GDD90 pKa = 3.47 ACLDD94 pKa = 3.26 EE95 pKa = 6.35 DD96 pKa = 5.35 YY97 pKa = 11.37 YY98 pKa = 11.48 DD99 pKa = 4.59 EE100 pKa = 4.7 PTLDD104 pKa = 3.89 SEE106 pKa = 4.67 DD107 pKa = 4.04 EE108 pKa = 4.25 RR109 pKa = 11.84 VQSYY113 pKa = 10.9 VDD115 pKa = 3.29 ACVSIMRR122 pKa = 11.84 EE123 pKa = 3.55 KK124 pKa = 10.92 CMASAAVADD133 pKa = 4.3 GVNCYY138 pKa = 10.21 RR139 pKa = 11.84 PNSPSNLSSASSNSWKK155 pKa = 10.6 FYY157 pKa = 8.65 DD158 pKa = 3.9 TRR160 pKa = 11.84 CLEE163 pKa = 4.08 SRR165 pKa = 11.84 VV166 pKa = 3.57
Molecular weight: 18.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.159
IPC2_protein 4.266
IPC_protein 4.202
Toseland 4.024
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.037
Rodwell 4.037
Grimsley 3.935
Solomon 4.151
Lehninger 4.101
Nozaki 4.266
DTASelect 4.431
Thurlkill 4.037
EMBOSS 4.05
Sillero 4.304
Patrickios 3.032
IPC_peptide 4.151
IPC2_peptide 4.291
IPC2.peptide.svr19 4.235
Protein with the highest isoelectric point:
>tr|Q1HTU4|Q1HTU4_9POXV Hypothetical pox protein OS=Squirrelpox virus OX=240426 GN=I6L PE=4 SV=1
MM1 pKa = 7.31 NGCFSSLSEE10 pKa = 4.2 SRR12 pKa = 11.84 LVTWVLRR19 pKa = 11.84 VTGQQRR25 pKa = 11.84 RR26 pKa = 11.84 LRR28 pKa = 11.84 RR29 pKa = 11.84 AKK31 pKa = 10.11 RR32 pKa = 11.84 RR33 pKa = 11.84 SRR35 pKa = 11.84 WIVGLDD41 pKa = 3.44 LEE43 pKa = 4.71 GGKK46 pKa = 9.75 VAVMDD51 pKa = 4.17 TYY53 pKa = 10.43 MEE55 pKa = 4.42 RR56 pKa = 11.84 FWPDD60 pKa = 3.88 GINLTLPRR68 pKa = 11.84 EE69 pKa = 4.2 DD70 pKa = 4.57 HH71 pKa = 6.38 PTPLPRR77 pKa = 11.84 KK78 pKa = 8.97 KK79 pKa = 10.18 KK80 pKa = 10.53 LKK82 pKa = 10.68 VKK84 pKa = 10.68 VKK86 pKa = 10.52 LL87 pKa = 3.68
Molecular weight: 10.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.254
IPC2_protein 9.853
IPC_protein 10.994
Toseland 11.301
ProMoST 11.535
Dawson 11.33
Bjellqvist 11.184
Wikipedia 11.667
Rodwell 11.418
Grimsley 11.359
Solomon 11.667
Lehninger 11.594
Nozaki 11.286
DTASelect 11.169
Thurlkill 11.286
EMBOSS 11.754
Sillero 11.286
Patrickios 11.155
IPC_peptide 11.681
IPC2_peptide 10.526
IPC2.peptide.svr19 9.081
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
141
0
141
46641
53
2060
330.8
36.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.472 ± 0.432
2.035 ± 0.108
6.141 ± 0.113
6.945 ± 0.508
4.003 ± 0.259
5.903 ± 0.406
2.125 ± 0.108
4.149 ± 0.261
4.014 ± 0.327
9.23 ± 0.253
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.556 ± 0.1
3.433 ± 0.201
4.794 ± 0.326
2.513 ± 0.186
8.244 ± 0.255
6.846 ± 0.251
5.412 ± 0.15
8.364 ± 0.258
0.718 ± 0.059
3.107 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here