Coccinia mosaic Tamil Nadu virus
Average proteome isoelectric point is 8.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076L608|A0A076L608_9GEMI Capsid protein OS=Coccinia mosaic Tamil Nadu virus OX=1532882 GN=AV1 PE=3 SV=1
MM1 pKa = 7.49 PRR3 pKa = 11.84 QRR5 pKa = 11.84 SFQIKK10 pKa = 9.71 AKK12 pKa = 10.49 NIFLTYY18 pKa = 8.78 PKK20 pKa = 10.44 CPIPKK25 pKa = 9.6 EE26 pKa = 3.9 QMLDD30 pKa = 2.76 ILKK33 pKa = 10.68 NIEE36 pKa = 4.33 CPSDD40 pKa = 3.2 KK41 pKa = 10.97 LFIRR45 pKa = 11.84 VAQEE49 pKa = 3.46 RR50 pKa = 11.84 HH51 pKa = 4.78 QDD53 pKa = 3.38 GSLHH57 pKa = 5.13 IHH59 pKa = 6.91 ALIQFKK65 pKa = 10.86 GKK67 pKa = 10.05 AQFRR71 pKa = 11.84 NPRR74 pKa = 11.84 HH75 pKa = 5.9 FDD77 pKa = 3.16 VVHH80 pKa = 6.13 PHH82 pKa = 6.67 SSTQFHH88 pKa = 7.2 PNVQGAKK95 pKa = 9.25 SCSDD99 pKa = 3.11 VKK101 pKa = 11.23 SYY103 pKa = 10.84 IEE105 pKa = 4.11 KK106 pKa = 10.91 DD107 pKa = 2.87 GDD109 pKa = 3.89 YY110 pKa = 11.12 VDD112 pKa = 3.43 WGEE115 pKa = 3.87 FQIDD119 pKa = 3.21 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTINDD132 pKa = 4.01 AAAEE136 pKa = 4.02 ALNSGSTEE144 pKa = 3.39 AAMNILRR151 pKa = 11.84 EE152 pKa = 4.32 KK153 pKa = 10.53 LPKK156 pKa = 10.25 DD157 pKa = 4.05 YY158 pKa = 10.76 IFQYY162 pKa = 10.97 HH163 pKa = 5.93 NYY165 pKa = 7.76 KK166 pKa = 10.03 TNLDD170 pKa = 4.25 RR171 pKa = 11.84 IFAAPVDD178 pKa = 4.13 TYY180 pKa = 11.38 VCPFSKK186 pKa = 10.5 EE187 pKa = 4.16 SFDD190 pKa = 3.73 LVPRR194 pKa = 11.84 EE195 pKa = 3.97 LVDD198 pKa = 3.21 WASEE202 pKa = 4.22 NIVCSAARR210 pKa = 11.84 PVRR213 pKa = 11.84 PIGIVLEE220 pKa = 4.47 GDD222 pKa = 3.33 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.37 LDD245 pKa = 4.41 LSPKK249 pKa = 10.0 IYY251 pKa = 11.0 SNDD254 pKa = 2.4 AWYY257 pKa = 10.92 NVIDD261 pKa = 5.2 DD262 pKa = 4.41 VDD264 pKa = 3.61 PHH266 pKa = 5.85 YY267 pKa = 11.0 LKK269 pKa = 10.7 HH270 pKa = 6.2 FKK272 pKa = 10.7 EE273 pKa = 4.46 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.47 YY287 pKa = 10.28 GKK289 pKa = 9.35 PVMIKK294 pKa = 10.55 GGIPTIFLCNPGPSSSYY311 pKa = 11.22 KK312 pKa = 10.6 EE313 pKa = 4.19 FLDD316 pKa = 3.54 EE317 pKa = 5.43 DD318 pKa = 4.1 KK319 pKa = 11.59 NSALKK324 pKa = 10.43 SWALKK329 pKa = 10.23 NAIFYY334 pKa = 9.32 TLEE337 pKa = 4.21 GPLYY341 pKa = 10.6 SGRR344 pKa = 11.84 QQNTEE349 pKa = 3.86 QEE351 pKa = 4.25 NEE353 pKa = 4.1 EE354 pKa = 4.39 VNSQEE359 pKa = 4.35 TNN361 pKa = 3.02
Molecular weight: 41.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.228
IPC2_protein 6.198
IPC_protein 6.313
Toseland 6.59
ProMoST 6.707
Dawson 6.678
Bjellqvist 6.634
Wikipedia 6.693
Rodwell 6.678
Grimsley 6.722
Solomon 6.693
Lehninger 6.678
Nozaki 6.956
DTASelect 7.117
Thurlkill 7.146
EMBOSS 7.132
Sillero 7.073
Patrickios 4.164
IPC_peptide 6.707
IPC2_peptide 6.868
IPC2.peptide.svr19 6.826
Protein with the highest isoelectric point:
>tr|A0A076LA79|A0A076LA79_9GEMI Nuclear shuttle protein OS=Coccinia mosaic Tamil Nadu virus OX=1532882 GN=BV1 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.79 SPYY27 pKa = 10.47 AVRR30 pKa = 11.84 AAVPIVRR37 pKa = 11.84 GTKK40 pKa = 9.45 ARR42 pKa = 11.84 AWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.87 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.06 SRR80 pKa = 11.84 HH81 pKa = 5.57 DD82 pKa = 3.47 VSHH85 pKa = 6.69 IGKK88 pKa = 8.76 VLCVSDD94 pKa = 3.57 VTRR97 pKa = 11.84 GTGLTHH103 pKa = 7.09 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.56 NHH131 pKa = 5.33 TNSVIFFLVRR141 pKa = 11.84 DD142 pKa = 3.99 RR143 pKa = 11.84 RR144 pKa = 11.84 PTGSPQDD151 pKa = 3.6 FGEE154 pKa = 4.28 VFNMFDD160 pKa = 4.35 SEE162 pKa = 4.37 PSTATVKK169 pKa = 10.38 NVHH172 pKa = 6.5 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 8.77 QVLRR180 pKa = 11.84 KK181 pKa = 9.12 WYY183 pKa = 8.16 STVTGGTYY191 pKa = 10.38 ASKK194 pKa = 10.48 EE195 pKa = 3.66 QALVKK200 pKa = 10.68 KK201 pKa = 9.39 FVRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 10.24 NQQEE215 pKa = 3.85 PGKK218 pKa = 10.12 YY219 pKa = 9.64 EE220 pKa = 3.87 NHH222 pKa = 6.31 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.575
IPC_protein 9.867
Toseland 10.145
ProMoST 9.882
Dawson 10.35
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.687
Grimsley 10.438
Solomon 10.379
Lehninger 10.335
Nozaki 10.16
DTASelect 10.043
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.277
Patrickios 10.204
IPC_peptide 10.379
IPC2_peptide 8.946
IPC2.peptide.svr19 8.55
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1613
85
361
201.6
22.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.774 ± 0.519
2.108 ± 0.333
5.27 ± 0.409
4.774 ± 0.455
3.968 ± 0.382
5.58 ± 0.411
3.1 ± 0.318
5.022 ± 0.57
5.952 ± 0.412
6.572 ± 0.634
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.294 ± 0.299
5.022 ± 0.459
5.518 ± 0.464
3.596 ± 0.575
7.502 ± 0.722
9.361 ± 0.885
6.758 ± 0.78
7.316 ± 0.982
1.302 ± 0.144
4.216 ± 0.395
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here