Amino acid dipepetide frequency for Propionibacteriaceae bacterium NML 130396

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.92AlaAla: 17.92 ± 0.195	1.048AlaCys: 1.048 ± 0.031	8.169AlaAsp: 8.169 ± 0.092	9.167AlaGlu: 9.167 ± 0.115	2.996AlaPhe: 2.996 ± 0.064	12.0AlaGly: 12.0 ± 0.122	2.43AlaHis: 2.43 ± 0.049	4.757AlaIle: 4.757 ± 0.076	2.812AlaLys: 2.812 ± 0.065	12.284AlaLeu: 12.284 ± 0.142	2.732AlaMet: 2.732 ± 0.049	2.412AlaAsn: 2.412 ± 0.048	6.014AlaPro: 6.014 ± 0.088	3.936AlaGln: 3.936 ± 0.068	8.948AlaArg: 8.948 ± 0.108	5.641AlaSer: 5.641 ± 0.071	6.964AlaThr: 6.964 ± 0.096	10.248AlaVal: 10.248 ± 0.11	1.934AlaTrp: 1.934 ± 0.045	2.826AlaTyr: 2.826 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.957CysAla: 0.957 ± 0.027	0.134CysCys: 0.134 ± 0.013	0.499CysAsp: 0.499 ± 0.024	0.419CysGlu: 0.419 ± 0.02	0.28CysPhe: 0.28 ± 0.016	1.014CysGly: 1.014 ± 0.031	0.216CysHis: 0.216 ± 0.014	0.248CysIle: 0.248 ± 0.014	0.103CysLys: 0.103 ± 0.009	0.749CysLeu: 0.749 ± 0.026	0.133CysMet: 0.133 ± 0.01	0.163CysAsn: 0.163 ± 0.011	0.524CysPro: 0.524 ± 0.025	0.254CysGln: 0.254 ± 0.017	0.666CysArg: 0.666 ± 0.024	0.471CysSer: 0.471 ± 0.019	0.568CysThr: 0.568 ± 0.024	0.645CysVal: 0.645 ± 0.021	0.156CysTrp: 0.156 ± 0.01	0.191CysTyr: 0.191 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.618AspAla: 6.618 ± 0.092	0.44AspCys: 0.44 ± 0.02	3.548AspAsp: 3.548 ± 0.066	4.08AspGlu: 4.08 ± 0.068	1.759AspPhe: 1.759 ± 0.041	5.53AspGly: 5.53 ± 0.082	1.417AspHis: 1.417 ± 0.039	1.698AspIle: 1.698 ± 0.047	1.334AspLys: 1.334 ± 0.046	6.335AspLeu: 6.335 ± 0.078	0.76AspMet: 0.76 ± 0.027	1.152AspAsn: 1.152 ± 0.041	4.639AspPro: 4.639 ± 0.07	2.282AspGln: 2.282 ± 0.039	5.169AspArg: 5.169 ± 0.086	2.726AspSer: 2.726 ± 0.048	2.848AspThr: 2.848 ± 0.051	4.076AspVal: 4.076 ± 0.065	1.016AspTrp: 1.016 ± 0.033	1.103AspTyr: 1.103 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.685GluAla: 6.685 ± 0.079	0.384GluCys: 0.384 ± 0.018	2.548GluAsp: 2.548 ± 0.051	3.025GluGlu: 3.025 ± 0.067	1.725GluPhe: 1.725 ± 0.04	3.912GluGly: 3.912 ± 0.056	1.581GluHis: 1.581 ± 0.038	2.341GluIle: 2.341 ± 0.053	1.408GluLys: 1.408 ± 0.042	7.769GluLeu: 7.769 ± 0.102	0.938GluMet: 0.938 ± 0.031	1.066GluAsn: 1.066 ± 0.035	3.213GluPro: 3.213 ± 0.061	3.067GluGln: 3.067 ± 0.064	5.359GluArg: 5.359 ± 0.075	2.731GluSer: 2.731 ± 0.051	2.722GluThr: 2.722 ± 0.046	5.564GluVal: 5.564 ± 0.079	0.864GluTrp: 0.864 ± 0.029	0.951GluTyr: 0.951 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	3.626PheAla: 3.626 ± 0.062	0.339PheCys: 0.339 ± 0.018	2.163PheAsp: 2.163 ± 0.051	1.491PheGlu: 1.491 ± 0.035	0.964PhePhe: 0.964 ± 0.036	3.343PheGly: 3.343 ± 0.058	0.554PheHis: 0.554 ± 0.021	0.919PheIle: 0.919 ± 0.03	0.559PheLys: 0.559 ± 0.023	2.595PheLeu: 2.595 ± 0.051	0.395PheMet: 0.395 ± 0.02	0.704PheAsn: 0.704 ± 0.029	1.328PhePro: 1.328 ± 0.034	0.77PheGln: 0.77 ± 0.03	1.75PheArg: 1.75 ± 0.04	1.556PheSer: 1.556 ± 0.035	1.975PheThr: 1.975 ± 0.045	2.442PheVal: 2.442 ± 0.05	0.508PheTrp: 0.508 ± 0.022	0.521PheTyr: 0.521 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	10.01GlyAla: 10.01 ± 0.112	0.974GlyCys: 0.974 ± 0.033	4.894GlyAsp: 4.894 ± 0.075	5.196GlyGlu: 5.196 ± 0.07	3.006GlyPhe: 3.006 ± 0.057	8.23GlyGly: 8.23 ± 0.136	2.036GlyHis: 2.036 ± 0.047	3.82GlyIle: 3.82 ± 0.067	2.363GlyLys: 2.363 ± 0.065	9.623GlyLeu: 9.623 ± 0.105	2.24GlyMet: 2.24 ± 0.049	1.973GlyAsn: 1.973 ± 0.044	4.529GlyPro: 4.529 ± 0.061	3.399GlyGln: 3.399 ± 0.089	7.326GlyArg: 7.326 ± 0.089	5.379GlySer: 5.379 ± 0.069	5.388GlyThr: 5.388 ± 0.08	7.982GlyVal: 7.982 ± 0.089	1.987GlyTrp: 1.987 ± 0.056	2.159GlyTyr: 2.159 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.161HisAla: 2.161 ± 0.051	0.215HisCys: 0.215 ± 0.016	1.347HisAsp: 1.347 ± 0.035	1.157HisGlu: 1.157 ± 0.035	0.622HisPhe: 0.622 ± 0.028	2.129HisGly: 2.129 ± 0.048	0.701HisHis: 0.701 ± 0.027	0.547HisIle: 0.547 ± 0.021	0.332HisLys: 0.332 ± 0.018	2.459HisLeu: 2.459 ± 0.047	0.287HisMet: 0.287 ± 0.017	0.426HisAsn: 0.426 ± 0.019	1.825HisPro: 1.825 ± 0.038	0.827HisGln: 0.827 ± 0.028	2.023HisArg: 2.023 ± 0.051	1.0HisSer: 1.0 ± 0.03	1.116HisThr: 1.116 ± 0.033	1.546HisVal: 1.546 ± 0.044	0.375HisTrp: 0.375 ± 0.018	0.488HisTyr: 0.488 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	5.23IleAla: 5.23 ± 0.082	0.418IleCys: 0.418 ± 0.019	2.784IleAsp: 2.784 ± 0.054	2.347IleGlu: 2.347 ± 0.045	1.011IlePhe: 1.011 ± 0.033	4.23IleGly: 4.23 ± 0.065	0.646IleHis: 0.646 ± 0.025	1.363IleIle: 1.363 ± 0.047	0.825IleLys: 0.825 ± 0.031	2.9IleLeu: 2.9 ± 0.054	0.56IleMet: 0.56 ± 0.023	0.902IleAsn: 0.902 ± 0.028	2.007IlePro: 2.007 ± 0.044	0.908IleGln: 0.908 ± 0.031	2.489IleArg: 2.489 ± 0.052	2.112IleSer: 2.112 ± 0.043	2.602IleThr: 2.602 ± 0.047	3.088IleVal: 3.088 ± 0.058	0.487IleTrp: 0.487 ± 0.023	0.669IleTyr: 0.669 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	2.584LysAla: 2.584 ± 0.056	0.129LysCys: 0.129 ± 0.011	1.135LysAsp: 1.135 ± 0.038	1.103LysGlu: 1.103 ± 0.038	0.596LysPhe: 0.596 ± 0.026	1.795LysGly: 1.795 ± 0.056	0.44LysHis: 0.44 ± 0.019	0.888LysIle: 0.888 ± 0.034	0.885LysLys: 0.885 ± 0.036	2.056LysLeu: 2.056 ± 0.052	0.435LysMet: 0.435 ± 0.024	0.559LysAsn: 0.559 ± 0.027	1.353LysPro: 1.353 ± 0.048	0.945LysGln: 0.945 ± 0.033	1.477LysArg: 1.477 ± 0.038	1.237LysSer: 1.237 ± 0.038	1.213LysThr: 1.213 ± 0.036	2.21LysVal: 2.21 ± 0.051	0.3LysTrp: 0.3 ± 0.018	0.459LysTyr: 0.459 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.804LeuAla: 14.804 ± 0.157	0.817LeuCys: 0.817 ± 0.027	6.221LeuAsp: 6.221 ± 0.09	5.022LeuGlu: 5.022 ± 0.074	2.635LeuPhe: 2.635 ± 0.062	9.857LeuGly: 9.857 ± 0.116	2.114LeuHis: 2.114 ± 0.042	4.004LeuIle: 4.004 ± 0.076	1.95LeuLys: 1.95 ± 0.055	11.139LeuLeu: 11.139 ± 0.169	1.791LeuMet: 1.791 ± 0.038	2.032LeuAsn: 2.032 ± 0.049	6.049LeuPro: 6.049 ± 0.079	2.934LeuGln: 2.934 ± 0.043	7.871LeuArg: 7.871 ± 0.093	5.554LeuSer: 5.554 ± 0.077	6.638LeuThr: 6.638 ± 0.092	9.756LeuVal: 9.756 ± 0.125	1.298LeuTrp: 1.298 ± 0.041	1.508LeuTyr: 1.508 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.516MetAla: 2.516 ± 0.054	0.137MetCys: 0.137 ± 0.011	0.909MetAsp: 0.909 ± 0.029	0.793MetGlu: 0.793 ± 0.028	0.513MetPhe: 0.513 ± 0.024	1.51MetGly: 1.51 ± 0.039	0.357MetHis: 0.357 ± 0.02	0.886MetIle: 0.886 ± 0.03	0.532MetLys: 0.532 ± 0.02	1.965MetLeu: 1.965 ± 0.039	0.389MetMet: 0.389 ± 0.021	0.502MetAsn: 0.502 ± 0.022	1.169MetPro: 1.169 ± 0.036	0.572MetGln: 0.572 ± 0.021	1.302MetArg: 1.302 ± 0.033	1.428MetSer: 1.428 ± 0.038	1.546MetThr: 1.546 ± 0.038	1.604MetVal: 1.604 ± 0.042	0.229MetTrp: 0.229 ± 0.016	0.284MetTyr: 0.284 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.396AsnAla: 2.396 ± 0.048	0.184AsnCys: 0.184 ± 0.014	1.174AsnAsp: 1.174 ± 0.032	1.051AsnGlu: 1.051 ± 0.027	0.668AsnPhe: 0.668 ± 0.025	1.966AsnGly: 1.966 ± 0.052	0.475AsnHis: 0.475 ± 0.023	0.676AsnIle: 0.676 ± 0.027	0.51AsnLys: 0.51 ± 0.025	2.123AsnLeu: 2.123 ± 0.041	0.337AsnMet: 0.337 ± 0.018	0.541AsnAsn: 0.541 ± 0.026	1.816AsnPro: 1.816 ± 0.041	0.843AsnGln: 0.843 ± 0.033	1.676AsnArg: 1.676 ± 0.039	1.048AsnSer: 1.048 ± 0.03	1.225AsnThr: 1.225 ± 0.038	1.495AsnVal: 1.495 ± 0.035	0.348AsnTrp: 0.348 ± 0.018	0.459AsnTyr: 0.459 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	7.306ProAla: 7.306 ± 0.1	0.315ProCys: 0.315 ± 0.017	3.99ProAsp: 3.99 ± 0.064	4.337ProGlu: 4.337 ± 0.067	1.481ProPhe: 1.481 ± 0.043	6.123ProGly: 6.123 ± 0.093	1.2ProHis: 1.2 ± 0.033	1.967ProIle: 1.967 ± 0.045	1.298ProLys: 1.298 ± 0.043	4.941ProLeu: 4.941 ± 0.07	1.174ProMet: 1.174 ± 0.035	1.224ProAsn: 1.224 ± 0.034	3.2ProPro: 3.2 ± 0.083	1.978ProGln: 1.978 ± 0.045	3.949ProArg: 3.949 ± 0.062	3.051ProSer: 3.051 ± 0.06	3.906ProThr: 3.906 ± 0.079	5.111ProVal: 5.111 ± 0.079	0.901ProTrp: 0.901 ± 0.025	1.011ProTyr: 1.011 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.285GlnAla: 4.285 ± 0.065	0.206GlnCys: 0.206 ± 0.012	1.265GlnAsp: 1.265 ± 0.034	1.417GlnGlu: 1.417 ± 0.038	0.812GlnPhe: 0.812 ± 0.032	2.671GlnGly: 2.671 ± 0.059	0.884GlnHis: 0.884 ± 0.033	1.338GlnIle: 1.338 ± 0.036	0.723GlnLys: 0.723 ± 0.029	4.166GlnLeu: 4.166 ± 0.064	0.591GlnMet: 0.591 ± 0.025	0.716GlnAsn: 0.716 ± 0.032	2.366GlnPro: 2.366 ± 0.065	2.0GlnGln: 2.0 ± 0.057	3.218GlnArg: 3.218 ± 0.062	1.5GlnSer: 1.5 ± 0.039	1.718GlnThr: 1.718 ± 0.042	3.597GlnVal: 3.597 ± 0.055	0.537GlnTrp: 0.537 ± 0.024	0.572GlnTyr: 0.572 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	8.886ArgAla: 8.886 ± 0.113	0.668ArgCys: 0.668 ± 0.025	4.116ArgAsp: 4.116 ± 0.064	4.462ArgGlu: 4.462 ± 0.065	2.532ArgPhe: 2.532 ± 0.046	6.006ArgGly: 6.006 ± 0.088	1.883ArgHis: 1.883 ± 0.046	3.438ArgIle: 3.438 ± 0.062	1.56ArgLys: 1.56 ± 0.047	8.39ArgLeu: 8.39 ± 0.109	2.038ArgMet: 2.038 ± 0.046	1.61ArgAsn: 1.61 ± 0.039	4.649ArgPro: 4.649 ± 0.076	2.728ArgGln: 2.728 ± 0.053	7.644ArgArg: 7.644 ± 0.113	4.204ArgSer: 4.204 ± 0.054	4.791ArgThr: 4.791 ± 0.073	6.257ArgVal: 6.257 ± 0.084	1.51ArgTrp: 1.51 ± 0.036	1.56ArgTyr: 1.56 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.422SerAla: 6.422 ± 0.082	0.429SerCys: 0.429 ± 0.021	2.896SerAsp: 2.896 ± 0.057	2.871SerGlu: 2.871 ± 0.049	1.603SerPhe: 1.603 ± 0.045	5.914SerGly: 5.914 ± 0.083	0.958SerHis: 0.958 ± 0.029	1.877SerIle: 1.877 ± 0.039	1.079SerLys: 1.079 ± 0.032	4.953SerLeu: 4.953 ± 0.073	1.203SerMet: 1.203 ± 0.033	1.076SerAsn: 1.076 ± 0.03	2.911SerPro: 2.911 ± 0.055	1.652SerGln: 1.652 ± 0.041	3.937SerArg: 3.937 ± 0.065	2.898SerSer: 2.898 ± 0.069	3.319SerThr: 3.319 ± 0.063	4.23SerVal: 4.23 ± 0.069	0.891SerTrp: 0.891 ± 0.03	1.027SerTyr: 1.027 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	7.207ThrAla: 7.207 ± 0.088	0.436ThrCys: 0.436 ± 0.025	3.742ThrAsp: 3.742 ± 0.056	3.598ThrGlu: 3.598 ± 0.062	1.685ThrPhe: 1.685 ± 0.041	6.092ThrGly: 6.092 ± 0.077	1.116ThrHis: 1.116 ± 0.034	2.285ThrIle: 2.285 ± 0.041	1.224ThrLys: 1.224 ± 0.037	5.534ThrLeu: 5.534 ± 0.076	1.11ThrMet: 1.11 ± 0.032	1.242ThrAsn: 1.242 ± 0.038	4.248ThrPro: 4.248 ± 0.082	1.505ThrGln: 1.505 ± 0.035	4.27ThrArg: 4.27 ± 0.068	3.164ThrSer: 3.164 ± 0.059	3.453ThrThr: 3.453 ± 0.064	5.25ThrVal: 5.25 ± 0.088	0.956ThrTrp: 0.956 ± 0.028	0.983ThrTyr: 0.983 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	10.878ValAla: 10.878 ± 0.117	0.762ValCys: 0.762 ± 0.033	5.32ValAsp: 5.32 ± 0.082	4.765ValGlu: 4.765 ± 0.062	2.383ValPhe: 2.383 ± 0.051	7.363ValGly: 7.363 ± 0.084	1.803ValHis: 1.803 ± 0.045	3.475ValIle: 3.475 ± 0.058	1.661ValLys: 1.661 ± 0.048	9.756ValLeu: 9.756 ± 0.114	1.536ValMet: 1.536 ± 0.039	1.921ValAsn: 1.921 ± 0.047	4.614ValPro: 4.614 ± 0.061	2.476ValGln: 2.476 ± 0.044	6.857ValArg: 6.857 ± 0.079	4.464ValSer: 4.464 ± 0.067	5.255ValThr: 5.255 ± 0.073	8.088ValVal: 8.088 ± 0.101	1.143ValTrp: 1.143 ± 0.033	1.239ValTyr: 1.239 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.723TrpAla: 1.723 ± 0.043	0.17TrpCys: 0.17 ± 0.014	0.718TrpAsp: 0.718 ± 0.026	0.653TrpGlu: 0.653 ± 0.024	0.599TrpPhe: 0.599 ± 0.022	1.173TrpGly: 1.173 ± 0.036	0.387TrpHis: 0.387 ± 0.021	0.601TrpIle: 0.601 ± 0.021	0.324TrpLys: 0.324 ± 0.017	2.121TrpLeu: 2.121 ± 0.051	0.295TrpMet: 0.295 ± 0.017	0.392TrpAsn: 0.392 ± 0.02	0.869TrpPro: 0.869 ± 0.033	0.769TrpGln: 0.769 ± 0.026	1.408TrpArg: 1.408 ± 0.042	1.097TrpSer: 1.097 ± 0.034	0.895TrpThr: 0.895 ± 0.03	1.253TrpVal: 1.253 ± 0.034	0.405TrpTrp: 0.405 ± 0.02	0.318TrpTyr: 0.318 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.023TyrAla: 3.023 ± 0.052	0.185TyrCys: 0.185 ± 0.014	1.092TyrAsp: 1.092 ± 0.038	0.898TyrGlu: 0.898 ± 0.03	0.632TyrPhe: 0.632 ± 0.022	1.657TyrGly: 1.657 ± 0.047	0.384TyrHis: 0.384 ± 0.018	0.452TyrIle: 0.452 ± 0.023	0.316TyrLys: 0.316 ± 0.019	2.137TyrLeu: 2.137 ± 0.048	0.202TyrMet: 0.202 ± 0.015	0.399TyrAsn: 0.399 ± 0.02	1.14TyrPro: 1.14 ± 0.038	0.74TyrGln: 0.74 ± 0.024	1.59TyrArg: 1.59 ± 0.043	0.885TyrSer: 0.885 ± 0.031	0.956TyrThr: 0.956 ± 0.038	1.314TyrVal: 1.314 ± 0.034	0.325TyrTrp: 0.325 ± 0.023	0.381TyrTyr: 0.381 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3444 proteins (1108311 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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