Vibrio phage YC
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 195 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A384ZS74|A0A384ZS74_9CAUD Uncharacterized protein OS=Vibrio phage YC OX=2267403 PE=4 SV=1
MM1 pKa = 6.42 TTFRR5 pKa = 11.84 NINISEE11 pKa = 4.29 HH12 pKa = 6.78 AGVDD16 pKa = 3.82 HH17 pKa = 7.49 IIVDD21 pKa = 4.23 LNGDD25 pKa = 4.02 LGYY28 pKa = 10.23 IEE30 pKa = 5.79 AYY32 pKa = 10.59 NEE34 pKa = 3.71 LLEE37 pKa = 4.77 WIGGAWLVIDD47 pKa = 4.64 CQTEE51 pKa = 4.19 AEE53 pKa = 4.68 DD54 pKa = 5.08 SLEE57 pKa = 3.81 QLLDD61 pKa = 3.56 SFQQCDD67 pKa = 3.7 EE68 pKa = 4.71 IIDD71 pKa = 3.79 YY72 pKa = 10.95 DD73 pKa = 4.48 KK74 pKa = 11.57 EE75 pKa = 4.22 EE76 pKa = 4.56 PEE78 pKa = 4.35 CGYY81 pKa = 8.89 TRR83 pKa = 11.84 YY84 pKa = 10.27 LVTIAEE90 pKa = 4.31
Molecular weight: 10.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.86
IPC2_protein 3.719
IPC_protein 3.643
Toseland 3.465
ProMoST 3.783
Dawson 3.63
Bjellqvist 3.846
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.376
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.91
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.77
Patrickios 0.172
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A384ZRZ7|A0A384ZRZ7_9CAUD Uncharacterized protein OS=Vibrio phage YC OX=2267403 PE=4 SV=1
MM1 pKa = 7.42 EE2 pKa = 5.58 MINVGRR8 pKa = 11.84 HH9 pKa = 5.36 RR10 pKa = 11.84 DD11 pKa = 3.82 DD12 pKa = 4.64 LGEE15 pKa = 4.27 DD16 pKa = 3.16 AWRR19 pKa = 11.84 VTCGGLVPVNTKK31 pKa = 10.29 RR32 pKa = 11.84 AVNHH36 pKa = 3.95 QTIWGQIRR44 pKa = 11.84 NVSYY48 pKa = 10.87 RR49 pKa = 11.84 NVVVWITLLTPNGEE63 pKa = 3.9 YY64 pKa = 10.68 DD65 pKa = 3.51 VAIDD69 pKa = 3.5 LRR71 pKa = 11.84 KK72 pKa = 10.02 VKK74 pKa = 10.2 KK75 pKa = 10.06 QRR77 pKa = 11.84 LKK79 pKa = 10.48 HH80 pKa = 5.91 LRR82 pKa = 11.84 AQGISTRR89 pKa = 11.84 PCGYY93 pKa = 8.3 TRR95 pKa = 11.84 YY96 pKa = 10.2 SYY98 pKa = 10.39 WEE100 pKa = 4.03 EE101 pKa = 4.26 GGGGLDD107 pKa = 2.89 TGAAHH112 pKa = 6.37 SHH114 pKa = 4.59 TMCRR118 pKa = 11.84 KK119 pKa = 6.91 TFRR122 pKa = 11.84 SMSTPARR129 pKa = 11.84 LFRR132 pKa = 11.84 WLNPFMWYY140 pKa = 10.33
Molecular weight: 16.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.472
IPC_protein 9.94
Toseland 10.014
ProMoST 9.853
Dawson 10.262
Bjellqvist 10.014
Wikipedia 10.482
Rodwell 10.467
Grimsley 10.35
Solomon 10.321
Lehninger 10.277
Nozaki 10.087
DTASelect 9.999
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.189
Patrickios 9.882
IPC_peptide 10.306
IPC2_peptide 9.092
IPC2.peptide.svr19 8.435
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
195
0
195
46541
46
1593
238.7
26.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.411 ± 0.146
1.126 ± 0.067
6.719 ± 0.143
7.176 ± 0.206
3.657 ± 0.101
6.835 ± 0.162
2.25 ± 0.103
5.853 ± 0.114
6.04 ± 0.25
8.045 ± 0.157
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.055 ± 0.107
4.895 ± 0.144
3.416 ± 0.111
3.431 ± 0.099
5.125 ± 0.13
6.323 ± 0.174
6.238 ± 0.21
7.387 ± 0.152
1.317 ± 0.065
3.702 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here