Capybara microvirus Cap3_SP_431
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5V6|A0A4P8W5V6_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_431 OX=2585450 PE=4 SV=1
MM1 pKa = 7.66 INEE4 pKa = 4.1 VTSIDD9 pKa = 3.88 NNNINNNIRR18 pKa = 11.84 DD19 pKa = 3.69 SATAGEE25 pKa = 5.07 LIDD28 pKa = 4.9 SPSMQNNGTAWLGTALAIAGLVGGTISTAVSNRR61 pKa = 11.84 KK62 pKa = 8.85 NRR64 pKa = 11.84 DD65 pKa = 3.26 YY66 pKa = 11.64 AEE68 pKa = 4.2 EE69 pKa = 3.98 QQEE72 pKa = 4.27 KK73 pKa = 10.02 ANEE76 pKa = 4.15 YY77 pKa = 7.66 NTPAQQRR84 pKa = 11.84 ARR86 pKa = 11.84 LEE88 pKa = 3.99 EE89 pKa = 4.56 AGINPLAGGMSNSLSQPVSSPNQDD113 pKa = 2.78 PTANAVSLASMLNGLALQKK132 pKa = 9.94 STIKK136 pKa = 10.24 LTDD139 pKa = 3.49 AQSEE143 pKa = 4.34 KK144 pKa = 10.12 MQAEE148 pKa = 4.17 TRR150 pKa = 11.84 NIEE153 pKa = 4.01 AQTASTSATLPFVLDD168 pKa = 4.24 DD169 pKa = 4.35 LQQKK173 pKa = 10.06 LLIGNSSLQLSKK185 pKa = 11.11 EE186 pKa = 3.65 QLDD189 pKa = 3.93 YY190 pKa = 11.54 VRR192 pKa = 11.84 SQTHH196 pKa = 6.99 LLNKK200 pKa = 9.1 QIEE203 pKa = 4.52 WFDD206 pKa = 3.44 QQQNRR211 pKa = 11.84 EE212 pKa = 4.14 SARR215 pKa = 11.84 LGLEE219 pKa = 3.61 QATAAFNRR227 pKa = 11.84 KK228 pKa = 8.11 NQTQLTEE235 pKa = 4.47 LEE237 pKa = 4.03 FAKK240 pKa = 10.71 QKK242 pKa = 8.59 FTEE245 pKa = 4.1 YY246 pKa = 10.62 LGEE249 pKa = 4.17 NEE251 pKa = 4.32 VAKK254 pKa = 10.17 TRR256 pKa = 11.84 AEE258 pKa = 3.85 NAKK261 pKa = 9.49 IQHH264 pKa = 6.14 EE265 pKa = 4.59 SDD267 pKa = 4.13 LLLQQFIQGEE277 pKa = 4.41 PLTRR281 pKa = 11.84 AYY283 pKa = 10.69 RR284 pKa = 11.84 NYY286 pKa = 10.73 ADD288 pKa = 4.11 AVYY291 pKa = 9.55 EE292 pKa = 4.24 SRR294 pKa = 11.84 VADD297 pKa = 3.31 IATEE301 pKa = 4.27 SIEE304 pKa = 4.15 SQGAKK309 pKa = 9.61 KK310 pKa = 10.12 AAEE313 pKa = 4.11 IKK315 pKa = 10.79 SSVKK319 pKa = 9.9 GSAYY323 pKa = 9.88 GKK325 pKa = 8.89 TMEE328 pKa = 4.33 YY329 pKa = 9.5 TNDD332 pKa = 3.54 VIEE335 pKa = 5.0 SIPLLGDD342 pKa = 3.55 LLKK345 pKa = 10.82 FLSKK349 pKa = 10.29 FAKK352 pKa = 10.2
Molecular weight: 38.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.066
IPC2_protein 5.067
IPC_protein 4.978
Toseland 4.851
ProMoST 5.054
Dawson 4.902
Bjellqvist 5.054
Wikipedia 4.736
Rodwell 4.825
Grimsley 4.762
Solomon 4.902
Lehninger 4.851
Nozaki 5.016
DTASelect 5.118
Thurlkill 4.838
EMBOSS 4.762
Sillero 5.092
Patrickios 4.38
IPC_peptide 4.914
IPC2_peptide 5.08
IPC2.peptide.svr19 5.14
Protein with the highest isoelectric point:
>tr|A0A4V1FVT2|A0A4V1FVT2_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_431 OX=2585450 PE=4 SV=1
MM1 pKa = 7.37 GKK3 pKa = 9.88 KK4 pKa = 10.07 VDD6 pKa = 4.21 DD7 pKa = 4.25 ATLSTCVGGKK17 pKa = 9.6 RR18 pKa = 11.84 FPPTSPIARR27 pKa = 11.84 SGPGGRR33 pKa = 11.84 LLSMCKK39 pKa = 9.84 GRR41 pKa = 11.84 QKK43 pKa = 10.7 GRR45 pKa = 11.84 EE46 pKa = 3.75 RR47 pKa = 11.84 NKK49 pKa = 10.54 RR50 pKa = 11.84 KK51 pKa = 9.92 SLPIKK56 pKa = 10.4 NKK58 pKa = 9.14 WSLKK62 pKa = 9.89 RR63 pKa = 11.84 DD64 pKa = 3.65 AEE66 pKa = 4.43 LGAHH70 pKa = 6.73 LAVDD74 pKa = 4.82 GVSEE78 pKa = 4.34 LVSQFLVFVRR88 pKa = 11.84 KK89 pKa = 10.31 SKK91 pKa = 11.26 DD92 pKa = 3.31 FLTKK96 pKa = 10.08 TSAWYY101 pKa = 9.39 QWVVV105 pKa = 2.77
Molecular weight: 11.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.238
IPC2_protein 9.633
IPC_protein 9.794
Toseland 10.877
ProMoST 10.365
Dawson 10.921
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.418
Grimsley 10.935
Solomon 10.994
Lehninger 10.994
Nozaki 10.847
DTASelect 10.511
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.847
Patrickios 11.169
IPC_peptide 11.008
IPC2_peptide 9.165
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1668
74
643
333.6
38.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.935 ± 1.776
1.559 ± 0.41
5.935 ± 0.562
5.096 ± 0.818
4.796 ± 0.711
5.396 ± 0.403
2.518 ± 0.983
5.336 ± 0.829
6.175 ± 0.831
10.192 ± 0.158
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.379 ± 0.152
7.134 ± 0.531
3.477 ± 0.353
4.676 ± 0.964
5.216 ± 0.432
7.914 ± 0.697
5.875 ± 0.858
5.036 ± 0.64
0.54 ± 0.245
5.815 ± 1.462
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here