Xanthomonas phage XaF13

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8FRS5|A0A5P8FRS5_9VIRU Major coat protein OS=Xanthomonas phage XaF13 OX=2608322 GN=XaF13_p03 PE=4 SV=1
MM1 pKa = 7.77AGGLRR6 pKa = 11.84DD7 pKa = 3.72CLRR10 pKa = 11.84GFRR13 pKa = 11.84IMGWLARR20 pKa = 11.84VFASAIARR28 pKa = 11.84RR29 pKa = 11.84LAYY32 pKa = 10.38VLVAATLAWCGMGKK46 pKa = 9.35AHH48 pKa = 7.67AANCDD53 pKa = 3.39ATSDD57 pKa = 3.67MCSEE61 pKa = 3.77GDD63 pKa = 2.99AWNAARR69 pKa = 11.84QLADD73 pKa = 3.03SRR75 pKa = 11.84GPDD78 pKa = 3.08TCKK81 pKa = 10.65LVGGNNASYY90 pKa = 9.64TGPNIEE96 pKa = 4.71KK97 pKa = 10.81DD98 pKa = 3.42PGNSSKK104 pKa = 11.1DD105 pKa = 3.4VGALAVRR112 pKa = 11.84VSCSNGSLPFLGTKK126 pKa = 10.27YY127 pKa = 10.58YY128 pKa = 9.54VTAKK132 pKa = 10.29SCEE135 pKa = 3.99QEE137 pKa = 3.52PGYY140 pKa = 9.61TGGGPWGTYY149 pKa = 9.82VGTARR154 pKa = 11.84NGSIGCRR161 pKa = 11.84NGCDD165 pKa = 3.55GVWFGNGDD173 pKa = 3.51GSMTWGSTGAVCPTDD188 pKa = 3.66PEE190 pKa = 4.5KK191 pKa = 10.67TCDD194 pKa = 4.08AMGKK198 pKa = 9.25GYY200 pKa = 10.47GWNGYY205 pKa = 9.99LGVCEE210 pKa = 4.44PPPTEE215 pKa = 4.26EE216 pKa = 4.33CPEE219 pKa = 4.09GQVPDD224 pKa = 4.14GKK226 pKa = 10.89GGCSTNKK233 pKa = 9.88CPEE236 pKa = 3.83GMLLQADD243 pKa = 5.09GTCTPKK249 pKa = 10.9KK250 pKa = 9.49NDD252 pKa = 4.1CPAGMIKK259 pKa = 10.3SPTGSCLPGDD269 pKa = 4.3GQCAAGEE276 pKa = 4.2VRR278 pKa = 11.84GPDD281 pKa = 3.6GTCKK285 pKa = 10.14KK286 pKa = 10.53DD287 pKa = 3.18ADD289 pKa = 4.38GDD291 pKa = 4.25GEE293 pKa = 4.38PDD295 pKa = 3.31GDD297 pKa = 4.09GEE299 pKa = 4.36GGEE302 pKa = 4.72GEE304 pKa = 4.43GDD306 pKa = 3.65SKK308 pKa = 11.79SEE310 pKa = 3.82FSGGDD315 pKa = 3.3SCDD318 pKa = 3.89SPPSCSGDD326 pKa = 3.87AIMCGQARR334 pKa = 11.84IQWRR338 pKa = 11.84IDD340 pKa = 3.14CNTRR344 pKa = 11.84RR345 pKa = 11.84DD346 pKa = 3.65VNITGGSCAAMPVCVGKK363 pKa = 10.03NCKK366 pKa = 9.7ALEE369 pKa = 3.9YY370 pKa = 10.64SQLLMQWRR378 pKa = 11.84AACALEE384 pKa = 4.09KK385 pKa = 10.52AANNSGGGTGNNADD399 pKa = 3.25VKK401 pKa = 10.58AIRR404 pKa = 11.84DD405 pKa = 4.1AITGNGSADD414 pKa = 2.91IGADD418 pKa = 3.62GKK420 pKa = 10.07PADD423 pKa = 4.27AFSDD427 pKa = 3.49EE428 pKa = 4.37SGYY431 pKa = 11.57GEE433 pKa = 4.85DD434 pKa = 4.73GYY436 pKa = 9.29PTGEE440 pKa = 5.23LDD442 pKa = 3.66TQGFGYY448 pKa = 10.49SRR450 pKa = 11.84TCPTIPDD457 pKa = 3.49VAVFGQTLHH466 pKa = 6.82FDD468 pKa = 3.53TSKK471 pKa = 9.79FCQWMVLGGQIVLVMASLVSLRR493 pKa = 11.84LMSQGGSAA501 pKa = 3.86

Molecular weight: 51.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8FT24|A0A5P8FT24_9VIRU Uncharacterized protein OS=Xanthomonas phage XaF13 OX=2608322 GN=XaF13_p09 PE=4 SV=1
MM1 pKa = 7.44TLFLAEE7 pKa = 3.77IRR9 pKa = 11.84LPGTLVAVGVSGFADD24 pKa = 3.62RR25 pKa = 11.84ALRR28 pKa = 11.84SVAIINDD35 pKa = 3.59HH36 pKa = 5.91SRR38 pKa = 11.84AMQQVSDD45 pKa = 4.16QNRR48 pKa = 11.84MSHH51 pKa = 6.16GSMVLPSGVKK61 pKa = 9.56KK62 pKa = 9.31WWPWKK67 pKa = 9.45PKK69 pKa = 9.17PAQGPARR76 pKa = 11.84STRR79 pKa = 11.84SS80 pKa = 3.08

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2239

46

501

159.9

17.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.166 ± 0.865

2.546 ± 0.897

4.913 ± 0.856

4.422 ± 0.656

2.858 ± 0.537

9.558 ± 1.791

1.787 ± 0.439

3.573 ± 0.579

4.377 ± 0.398

8.575 ± 1.463

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.373

2.858 ± 0.415

6.253 ± 0.699

4.332 ± 0.73

6.744 ± 0.842

6.61 ± 0.694

5.404 ± 0.588

6.342 ± 0.743

2.903 ± 0.601

2.01 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski