Nonomuraea fuscirosea

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0MPS6|A0A2T0MPS6_9ACTN Xylose isomerase OS=Nonomuraea fuscirosea OX=1291556 GN=xylA PE=3 SV=1
MM1 pKa = 7.1WWGTAIEE8 pKa = 4.68APDD11 pKa = 4.08PAALAQFYY19 pKa = 10.78SDD21 pKa = 3.91LLGWPIGHH29 pKa = 5.99QEE31 pKa = 4.08PGTTILAAPEE41 pKa = 3.74GSIYY45 pKa = 10.32IVFQQATDD53 pKa = 3.6YY54 pKa = 10.02QAPVWPPAAGQQRR67 pKa = 11.84PMMHH71 pKa = 7.14FDD73 pKa = 3.66FQVGDD78 pKa = 3.86LDD80 pKa = 4.06SAVAEE85 pKa = 4.3AVALGASVATEE96 pKa = 3.84QPQDD100 pKa = 3.36NVRR103 pKa = 11.84VLFDD107 pKa = 3.71PAGHH111 pKa = 6.94PFCLCRR117 pKa = 11.84DD118 pKa = 3.93DD119 pKa = 4.52GG120 pKa = 4.15

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0N9I1|A0A2T0N9I1_9ACTN Acetyl coenzyme A synthetase (ADP forming)-like protein OS=Nonomuraea fuscirosea OX=1291556 GN=B0I32_102427 PE=4 SV=1
MM1 pKa = 7.6ARR3 pKa = 11.84PPTAPVRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84FPPPRR17 pKa = 11.84VRR19 pKa = 11.84ARR21 pKa = 11.84SPVRR25 pKa = 11.84VPPPQGAPPVAARR38 pKa = 11.84SSARR42 pKa = 11.84RR43 pKa = 11.84WRR45 pKa = 11.84CRR47 pKa = 11.84TSPSRR52 pKa = 11.84SLRR55 pKa = 11.84PPAARR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84GLRR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84VSRR70 pKa = 11.84AARR73 pKa = 11.84PLVRR77 pKa = 11.84RR78 pKa = 11.84AASPLVPLRR87 pKa = 11.84ARR89 pKa = 11.84GPVPLLVRR97 pKa = 11.84APGPAPRR104 pKa = 11.84PVPGPRR110 pKa = 11.84AAAVASSRR118 pKa = 11.84LRR120 pKa = 11.84VPAAAVARR128 pKa = 11.84VRR130 pKa = 11.84DD131 pKa = 4.19RR132 pKa = 11.84VPSPARR138 pKa = 11.84VVRR141 pKa = 11.84VPATTRR147 pKa = 11.84SRR149 pKa = 11.84PTPAAWASPGRR160 pKa = 11.84RR161 pKa = 11.84VLAATARR168 pKa = 11.84AVAAPASVSVVTVRR182 pKa = 11.84RR183 pKa = 11.84ATARR187 pKa = 11.84CRR189 pKa = 11.84VRR191 pKa = 11.84RR192 pKa = 11.84RR193 pKa = 11.84VVAAARR199 pKa = 11.84AGPAAPVRR207 pKa = 11.84VLDD210 pKa = 4.1RR211 pKa = 11.84PPAARR216 pKa = 11.84VPVLVPVVPVLVARR230 pKa = 11.84GPTRR234 pKa = 3.29

Molecular weight: 24.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11276

0

11276

3590346

25

3748

318.4

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.349 ± 0.033

0.778 ± 0.007

5.733 ± 0.018

5.585 ± 0.021

2.814 ± 0.013

9.466 ± 0.023

2.223 ± 0.014

3.499 ± 0.015

1.987 ± 0.016

10.635 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.884 ± 0.008

1.753 ± 0.012

6.098 ± 0.019

2.704 ± 0.014

8.283 ± 0.026

5.023 ± 0.017

5.881 ± 0.021

8.578 ± 0.02

1.593 ± 0.01

2.134 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski