Frankia sp. (strain EAN1pec)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia; unclassified Frankia

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8LAM3|A8LAM3_FRASN 40-residue YVTN family beta-propeller repeat protein OS=Frankia sp. (strain EAN1pec) OX=298653 GN=Franean1_4419 PE=4 SV=1
MM1 pKa = 7.87PEE3 pKa = 4.37DD4 pKa = 4.18SDD6 pKa = 5.32TILLDD11 pKa = 3.67YY12 pKa = 11.06ALSTDD17 pKa = 4.03PAPVPASTAQVVNQARR33 pKa = 11.84VNIGVSAEE41 pKa = 4.1TTVYY45 pKa = 10.43CSQIVIAVPIGTDD58 pKa = 3.46DD59 pKa = 3.7ASLYY63 pKa = 10.96ALAPAGSSNSNKK75 pKa = 9.21WAVTAQLRR83 pKa = 11.84KK84 pKa = 10.24GRR86 pKa = 11.84DD87 pKa = 2.96LWDD90 pKa = 4.41GLDD93 pKa = 3.5EE94 pKa = 4.8DD95 pKa = 3.96QDD97 pKa = 4.12YY98 pKa = 8.76STFVYY103 pKa = 10.59DD104 pKa = 4.36CVSSTDD110 pKa = 3.22YY111 pKa = 10.99RR112 pKa = 11.84VDD114 pKa = 3.4YY115 pKa = 10.96NLVFGVFGAVNQTVGDD131 pKa = 3.93AEE133 pKa = 4.12IAIQEE138 pKa = 4.28TSGTSSDD145 pKa = 3.39PTTFTLKK152 pKa = 10.1PAYY155 pKa = 10.19FPVRR159 pKa = 11.84KK160 pKa = 8.74EE161 pKa = 3.55WPAFYY166 pKa = 10.66LKK168 pKa = 10.83NLVAATLASPTVPATDD184 pKa = 4.11FSEE187 pKa = 4.53GDD189 pKa = 3.55PVRR192 pKa = 11.84LSWEE196 pKa = 4.1SNGTYY201 pKa = 9.92FQLFTKK207 pKa = 9.84GQSRR211 pKa = 11.84PVYY214 pKa = 10.11AGTATTYY221 pKa = 9.93TLEE224 pKa = 4.45EE225 pKa = 4.35GLSRR229 pKa = 11.84DD230 pKa = 3.32TAFVLTASMTGSPDD244 pKa = 3.06EE245 pKa = 5.18DD246 pKa = 4.05VPQGGYY252 pKa = 10.16EE253 pKa = 4.13PIYY256 pKa = 10.49LYY258 pKa = 10.83DD259 pKa = 4.25ALTVSVSNPVLTPSSVTVSGTLEE282 pKa = 3.97VSGTSYY288 pKa = 11.53LSTTEE293 pKa = 3.95TGSLSSSSVSVNGSLDD309 pKa = 3.46VDD311 pKa = 4.29GSTSLSDD318 pKa = 3.29TTVDD322 pKa = 4.05DD323 pKa = 4.19GLTVRR328 pKa = 11.84GSTEE332 pKa = 3.94LEE334 pKa = 3.85EE335 pKa = 4.34SLSVSGSTSLSDD347 pKa = 3.32ATVTGTLSGTGSASLSDD364 pKa = 3.32LTVRR368 pKa = 11.84GLYY371 pKa = 10.23GYY373 pKa = 9.6SGRR376 pKa = 11.84VEE378 pKa = 4.06LLGNANLVDD387 pKa = 3.57QGANLGQTGVYY398 pKa = 8.12TATDD402 pKa = 4.12GFAVAQVLTPSDD414 pKa = 3.67NSQASYY420 pKa = 10.84AYY422 pKa = 9.47GWIYY426 pKa = 10.69TSGTWFGVQGGTVGSFGPGWSDD448 pKa = 4.46FMNTNPNTITVPIPAGSYY466 pKa = 8.52WYY468 pKa = 10.35YY469 pKa = 11.2EE470 pKa = 4.09SFNPSNNQVYY480 pKa = 10.85SDD482 pKa = 3.65VQFWWFPLGSDD493 pKa = 3.54YY494 pKa = 11.29GATATYY500 pKa = 10.41RR501 pKa = 11.84KK502 pKa = 8.31LTAEE506 pKa = 4.07EE507 pKa = 4.14RR508 pKa = 11.84VAEE511 pKa = 4.15QVEE514 pKa = 4.66VPPARR519 pKa = 11.84PAVPDD524 pKa = 3.29IAAAVEE530 pKa = 4.05RR531 pKa = 11.84RR532 pKa = 11.84EE533 pKa = 3.77NAAAAFVEE541 pKa = 4.42RR542 pKa = 11.84LAAALEE548 pKa = 4.14ADD550 pKa = 3.62LAEE553 pKa = 4.52EE554 pKa = 4.28VRR556 pKa = 11.84ADD558 pKa = 3.59LTRR561 pKa = 11.84LLNQHH566 pKa = 6.3

Molecular weight: 60.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8L989|A8L989_FRASN Uncharacterized protein OS=Frankia sp. (strain EAN1pec) OX=298653 GN=Franean1_2805 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.69GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.93GRR40 pKa = 11.84SQLSAA45 pKa = 3.6

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7133

0

7133

2476290

26

6999

347.2

36.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.01 ± 0.053

0.797 ± 0.008

6.129 ± 0.02

5.064 ± 0.026

2.631 ± 0.016

9.698 ± 0.038

2.226 ± 0.014

3.358 ± 0.019

1.396 ± 0.015

10.048 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.633 ± 0.013

1.67 ± 0.013

6.705 ± 0.032

2.585 ± 0.016

8.802 ± 0.03

5.283 ± 0.022

6.098 ± 0.021

8.584 ± 0.03

1.451 ± 0.012

1.832 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski