Sulfuricurvum sp. MLSB
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2005 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A091ANL1|A0A091ANL1_9PROT Protease HtpX homolog OS=Sulfuricurvum sp. MLSB OX=1537917 GN=htpX PE=3 SV=1
MM1 pKa = 7.23 MNATKK6 pKa = 10.42 LRR8 pKa = 11.84 VMQAIFSLSDD18 pKa = 3.04 EE19 pKa = 4.49 TEE21 pKa = 4.13 YY22 pKa = 11.36 NIYY25 pKa = 10.25 EE26 pKa = 4.22 ADD28 pKa = 5.09 DD29 pKa = 3.36 IAEE32 pKa = 4.33 YY33 pKa = 11.26 ARR35 pKa = 11.84 MDD37 pKa = 3.88 ADD39 pKa = 3.45 QVRR42 pKa = 11.84 TIVSEE47 pKa = 4.39 LYY49 pKa = 10.53 DD50 pKa = 3.43 EE51 pKa = 5.92 GYY53 pKa = 10.68 LGEE56 pKa = 4.72 CMSIGDD62 pKa = 4.17 DD63 pKa = 4.04 GYY65 pKa = 10.7 EE66 pKa = 3.73 TFYY69 pKa = 11.32 LNKK72 pKa = 9.93 KK73 pKa = 8.88 GRR75 pKa = 11.84 ALIGMEE81 pKa = 3.93
Molecular weight: 9.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.833
IPC2_protein 4.088
IPC_protein 3.999
Toseland 3.808
ProMoST 4.088
Dawson 3.961
Bjellqvist 4.19
Wikipedia 3.872
Rodwell 3.834
Grimsley 3.719
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.253
Thurlkill 3.846
EMBOSS 3.884
Sillero 4.113
Patrickios 1.926
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.034
Protein with the highest isoelectric point:
>tr|A0A091AM83|A0A091AM83_9PROT DNA polymerase III subunit gamma/tau OS=Sulfuricurvum sp. MLSB OX=1537917 GN=dnaX PE=3 SV=1
MM1 pKa = 7.21 TKK3 pKa = 10.08 SEE5 pKa = 4.11 FRR7 pKa = 11.84 SEE9 pKa = 3.94 CTHH12 pKa = 6.49 RR13 pKa = 11.84 LKK15 pKa = 10.85 NANRR19 pKa = 11.84 STKK22 pKa = 10.19 RR23 pKa = 11.84 IRR25 pKa = 11.84 DD26 pKa = 3.53 AKK28 pKa = 8.81 VRR30 pKa = 11.84 RR31 pKa = 11.84 MLQIVLKK38 pKa = 9.69 EE39 pKa = 4.0 LGPKK43 pKa = 9.58 RR44 pKa = 11.84 VLAFWPMEE52 pKa = 3.89 FEE54 pKa = 3.96 ADD56 pKa = 3.14 IRR58 pKa = 11.84 KK59 pKa = 9.65 SLTTMRR65 pKa = 11.84 RR66 pKa = 11.84 WDD68 pKa = 3.56 KK69 pKa = 11.27 VYY71 pKa = 10.83 LPFMDD76 pKa = 4.02 GVSFKK81 pKa = 10.05 MVPLRR86 pKa = 11.84 LPLEE90 pKa = 4.25 RR91 pKa = 11.84 KK92 pKa = 9.81 AFGIYY97 pKa = 9.43 EE98 pKa = 4.12 PRR100 pKa = 11.84 DD101 pKa = 3.43 SFRR104 pKa = 11.84 KK105 pKa = 9.64 INLIDD110 pKa = 3.66 VAIVPAIGVDD120 pKa = 3.39 ASLGRR125 pKa = 11.84 IGFGKK130 pKa = 10.53 GMYY133 pKa = 10.21 DD134 pKa = 3.9 RR135 pKa = 11.84 FFPTLKK141 pKa = 9.52 TKK143 pKa = 10.42 PFTIFIQSCEE153 pKa = 3.79 CRR155 pKa = 11.84 TNRR158 pKa = 11.84 FLCDD162 pKa = 3.93 DD163 pKa = 3.82 YY164 pKa = 11.86 DD165 pKa = 3.75 VRR167 pKa = 11.84 SDD169 pKa = 4.93 LLITPKK175 pKa = 10.5 GVYY178 pKa = 9.61 SMRR181 pKa = 11.84 KK182 pKa = 5.42 THH184 pKa = 6.0 VKK186 pKa = 8.75 RR187 pKa = 11.84 TTRR190 pKa = 11.84 GGRR193 pKa = 11.84 HH194 pKa = 5.11 SHH196 pKa = 6.14 LQRR199 pKa = 11.84 CSRR202 pKa = 11.84 LFNFEE207 pKa = 3.41 KK208 pKa = 10.68 DD209 pKa = 3.5 FRR211 pKa = 11.84 RR212 pKa = 11.84 SFF214 pKa = 3.32
Molecular weight: 25.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.604
IPC_protein 10.16
Toseland 10.745
ProMoST 10.35
Dawson 10.818
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 11.111
Grimsley 10.847
Solomon 10.921
Lehninger 10.891
Nozaki 10.73
DTASelect 10.482
Thurlkill 10.73
EMBOSS 11.125
Sillero 10.745
Patrickios 10.833
IPC_peptide 10.921
IPC2_peptide 9.428
IPC2.peptide.svr19 8.688
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2005
0
2005
577772
39
1558
288.2
32.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.426 ± 0.059
0.98 ± 0.024
5.529 ± 0.043
7.181 ± 0.059
4.513 ± 0.041
6.698 ± 0.051
2.307 ± 0.027
7.436 ± 0.054
6.211 ± 0.052
9.759 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.813 ± 0.029
4.146 ± 0.045
3.582 ± 0.032
3.076 ± 0.029
4.812 ± 0.039
6.266 ± 0.04
5.239 ± 0.045
6.549 ± 0.045
0.83 ± 0.019
3.647 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here