Amino acid dipepetide frequency for Pedobacter steynii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.523AlaAla: 6.523 ± 0.076	0.605AlaCys: 0.605 ± 0.017	4.249AlaAsp: 4.249 ± 0.048	4.608AlaGlu: 4.608 ± 0.058	3.49AlaPhe: 3.49 ± 0.043	5.784AlaGly: 5.784 ± 0.091	1.165AlaHis: 1.165 ± 0.028	5.216AlaIle: 5.216 ± 0.06	4.578AlaLys: 4.578 ± 0.056	7.068AlaLeu: 7.068 ± 0.078	1.677AlaMet: 1.677 ± 0.032	3.609AlaAsn: 3.609 ± 0.06	2.32AlaPro: 2.32 ± 0.045	2.725AlaGln: 2.725 ± 0.037	2.464AlaArg: 2.464 ± 0.037	4.653AlaSer: 4.653 ± 0.069	4.109AlaThr: 4.109 ± 0.124	4.634AlaVal: 4.634 ± 0.061	0.763AlaTrp: 0.763 ± 0.018	3.023AlaTyr: 3.023 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.46CysAla: 0.46 ± 0.019	0.117CysCys: 0.117 ± 0.008	0.352CysAsp: 0.352 ± 0.016	0.366CysGlu: 0.366 ± 0.018	0.455CysPhe: 0.455 ± 0.024	0.564CysGly: 0.564 ± 0.018	0.18CysHis: 0.18 ± 0.011	0.577CysIle: 0.577 ± 0.019	0.491CysLys: 0.491 ± 0.017	0.774CysLeu: 0.774 ± 0.022	0.172CysMet: 0.172 ± 0.01	0.349CysAsn: 0.349 ± 0.013	0.278CysPro: 0.278 ± 0.012	0.222CysGln: 0.222 ± 0.011	0.3CysArg: 0.3 ± 0.011	0.531CysSer: 0.531 ± 0.021	0.38CysThr: 0.38 ± 0.015	0.414CysVal: 0.414 ± 0.017	0.077CysTrp: 0.077 ± 0.006	0.283CysTyr: 0.283 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.777AspAla: 3.777 ± 0.049	0.342AspCys: 0.342 ± 0.014	2.258AspAsp: 2.258 ± 0.036	3.13AspGlu: 3.13 ± 0.045	3.109AspPhe: 3.109 ± 0.043	3.723AspGly: 3.723 ± 0.048	1.19AspHis: 1.19 ± 0.026	3.715AspIle: 3.715 ± 0.045	3.722AspLys: 3.722 ± 0.046	5.428AspLeu: 5.428 ± 0.061	1.101AspMet: 1.101 ± 0.027	2.331AspAsn: 2.331 ± 0.042	2.27AspPro: 2.27 ± 0.037	2.398AspGln: 2.398 ± 0.033	2.16AspArg: 2.16 ± 0.037	2.687AspSer: 2.687 ± 0.046	2.345AspThr: 2.345 ± 0.039	3.165AspVal: 3.165 ± 0.045	0.794AspTrp: 0.794 ± 0.021	2.507AspTyr: 2.507 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	4.17GluAla: 4.17 ± 0.057	0.302GluCys: 0.302 ± 0.012	2.752GluAsp: 2.752 ± 0.05	4.244GluGlu: 4.244 ± 0.07	2.464GluPhe: 2.464 ± 0.036	3.58GluGly: 3.58 ± 0.056	1.12GluHis: 1.12 ± 0.025	4.522GluIle: 4.522 ± 0.056	4.902GluLys: 4.902 ± 0.064	6.151GluLeu: 6.151 ± 0.071	1.52GluMet: 1.52 ± 0.032	3.459GluAsn: 3.459 ± 0.042	1.617GluPro: 1.617 ± 0.03	2.651GluGln: 2.651 ± 0.043	2.674GluArg: 2.674 ± 0.042	3.143GluSer: 3.143 ± 0.044	2.988GluThr: 2.988 ± 0.041	4.0GluVal: 4.0 ± 0.057	0.677GluTrp: 0.677 ± 0.02	2.068GluTyr: 2.068 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.137PheAla: 3.137 ± 0.042	0.483PheCys: 0.483 ± 0.028	2.844PheAsp: 2.844 ± 0.042	2.766PheGlu: 2.766 ± 0.04	2.456PhePhe: 2.456 ± 0.045	3.403PheGly: 3.403 ± 0.048	0.817PheHis: 0.817 ± 0.022	3.453PheIle: 3.453 ± 0.055	3.348PheLys: 3.348 ± 0.041	4.548PheLeu: 4.548 ± 0.065	1.113PheMet: 1.113 ± 0.027	3.08PheAsn: 3.08 ± 0.045	1.779PhePro: 1.779 ± 0.032	1.446PheGln: 1.446 ± 0.027	1.917PheArg: 1.917 ± 0.035	4.173PheSer: 4.173 ± 0.052	2.847PheThr: 2.847 ± 0.05	2.727PheVal: 2.727 ± 0.041	0.583PheTrp: 0.583 ± 0.018	2.144PheTyr: 2.144 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	4.763GlyAla: 4.763 ± 0.077	0.585GlyCys: 0.585 ± 0.022	3.241GlyAsp: 3.241 ± 0.048	3.408GlyGlu: 3.408 ± 0.041	3.628GlyPhe: 3.628 ± 0.054	4.897GlyGly: 4.897 ± 0.067	1.154GlyHis: 1.154 ± 0.029	5.376GlyIle: 5.376 ± 0.061	5.627GlyLys: 5.627 ± 0.061	6.752GlyLeu: 6.752 ± 0.067	1.706GlyMet: 1.706 ± 0.033	4.011GlyAsn: 4.011 ± 0.083	1.716GlyPro: 1.716 ± 0.039	2.36GlyGln: 2.36 ± 0.037	2.558GlyArg: 2.558 ± 0.035	4.57GlySer: 4.57 ± 0.064	4.562GlyThr: 4.562 ± 0.115	4.298GlyVal: 4.298 ± 0.054	0.938GlyTrp: 0.938 ± 0.024	3.194GlyTyr: 3.194 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.073HisAla: 1.073 ± 0.026	0.188HisCys: 0.188 ± 0.01	0.804HisAsp: 0.804 ± 0.023	0.953HisGlu: 0.953 ± 0.025	1.108HisPhe: 1.108 ± 0.026	1.051HisGly: 1.051 ± 0.023	0.56HisHis: 0.56 ± 0.018	1.294HisIle: 1.294 ± 0.031	1.067HisLys: 1.067 ± 0.029	1.923HisLeu: 1.923 ± 0.041	0.354HisMet: 0.354 ± 0.014	0.812HisAsn: 0.812 ± 0.022	0.958HisPro: 0.958 ± 0.021	0.896HisGln: 0.896 ± 0.025	0.735HisArg: 0.735 ± 0.018	1.051HisSer: 1.051 ± 0.028	0.959HisThr: 0.959 ± 0.024	0.898HisVal: 0.898 ± 0.026	0.225HisTrp: 0.225 ± 0.011	0.835HisTyr: 0.835 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.631IleAla: 5.631 ± 0.059	0.665IleCys: 0.665 ± 0.02	4.013IleAsp: 4.013 ± 0.048	4.163IleGlu: 4.163 ± 0.057	2.962IlePhe: 2.962 ± 0.047	4.913IleGly: 4.913 ± 0.052	1.286IleHis: 1.286 ± 0.028	4.602IleIle: 4.602 ± 0.067	4.896IleLys: 4.896 ± 0.058	6.414IleLeu: 6.414 ± 0.075	1.295IleMet: 1.295 ± 0.03	4.234IleAsn: 4.234 ± 0.052	3.252IlePro: 3.252 ± 0.045	2.356IleGln: 2.356 ± 0.035	3.163IleArg: 3.163 ± 0.042	5.647IleSer: 5.647 ± 0.06	4.379IleThr: 4.379 ± 0.088	3.999IleVal: 3.999 ± 0.055	0.732IleTrp: 0.732 ± 0.022	2.702IleTyr: 2.702 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	5.165LysAla: 5.165 ± 0.059	0.292LysCys: 0.292 ± 0.014	4.311LysAsp: 4.311 ± 0.061	5.158LysGlu: 5.158 ± 0.061	2.633LysPhe: 2.633 ± 0.035	4.849LysGly: 4.849 ± 0.051	1.238LysHis: 1.238 ± 0.029	5.148LysIle: 5.148 ± 0.058	5.616LysLys: 5.616 ± 0.075	6.128LysLeu: 6.128 ± 0.06	1.937LysMet: 1.937 ± 0.034	4.26LysAsn: 4.26 ± 0.046	2.634LysPro: 2.634 ± 0.041	2.592LysGln: 2.592 ± 0.044	2.771LysArg: 2.771 ± 0.043	4.265LysSer: 4.265 ± 0.052	4.275LysThr: 4.275 ± 0.056	4.611LysVal: 4.611 ± 0.055	0.813LysTrp: 0.813 ± 0.019	2.789LysTyr: 2.789 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	6.705LeuAla: 6.705 ± 0.075	0.777LeuCys: 0.777 ± 0.023	4.786LeuAsp: 4.786 ± 0.05	5.202LeuGlu: 5.202 ± 0.069	4.872LeuPhe: 4.872 ± 0.066	5.796LeuGly: 5.796 ± 0.067	1.587LeuHis: 1.587 ± 0.034	6.992LeuIle: 6.992 ± 0.083	7.931LeuLys: 7.931 ± 0.08	9.792LeuLeu: 9.792 ± 0.107	2.317LeuMet: 2.317 ± 0.036	6.237LeuAsn: 6.237 ± 0.067	4.132LeuPro: 4.132 ± 0.052	3.633LeuGln: 3.633 ± 0.048	3.802LeuArg: 3.802 ± 0.046	8.018LeuSer: 8.018 ± 0.085	5.542LeuThr: 5.542 ± 0.074	5.246LeuVal: 5.246 ± 0.053	0.948LeuTrp: 0.948 ± 0.026	3.288LeuTyr: 3.288 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	1.791MetAla: 1.791 ± 0.031	0.118MetCys: 0.118 ± 0.008	1.22MetAsp: 1.22 ± 0.03	1.502MetGlu: 1.502 ± 0.035	0.812MetPhe: 0.812 ± 0.018	1.507MetGly: 1.507 ± 0.029	0.361MetHis: 0.361 ± 0.014	1.604MetIle: 1.604 ± 0.032	2.023MetLys: 2.023 ± 0.033	2.145MetLeu: 2.145 ± 0.037	0.661MetMet: 0.661 ± 0.02	1.37MetAsn: 1.37 ± 0.028	1.047MetPro: 1.047 ± 0.023	0.857MetGln: 0.857 ± 0.021	0.911MetArg: 0.911 ± 0.022	1.368MetSer: 1.368 ± 0.026	1.11MetThr: 1.11 ± 0.026	1.477MetVal: 1.477 ± 0.031	0.167MetTrp: 0.167 ± 0.01	0.653MetTyr: 0.653 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	4.053AsnAla: 4.053 ± 0.06	0.392AsnCys: 0.392 ± 0.014	2.712AsnAsp: 2.712 ± 0.04	3.121AsnGlu: 3.121 ± 0.04	2.694AsnPhe: 2.694 ± 0.039	4.249AsnGly: 4.249 ± 0.064	0.981AsnHis: 0.981 ± 0.025	4.001AsnIle: 4.001 ± 0.05	3.724AsnLys: 3.724 ± 0.043	5.147AsnLeu: 5.147 ± 0.058	1.191AsnMet: 1.191 ± 0.022	3.171AsnAsn: 3.171 ± 0.055	2.889AsnPro: 2.889 ± 0.06	2.106AsnGln: 2.106 ± 0.04	2.353AsnArg: 2.353 ± 0.035	3.501AsnSer: 3.501 ± 0.055	3.364AsnThr: 3.364 ± 0.057	3.159AsnVal: 3.159 ± 0.055	0.775AsnTrp: 0.775 ± 0.024	2.678AsnTyr: 2.678 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	3.26ProAla: 3.26 ± 0.066	0.227ProCys: 0.227 ± 0.011	2.567ProAsp: 2.567 ± 0.034	3.12ProGlu: 3.12 ± 0.046	1.95ProPhe: 1.95 ± 0.032	3.012ProGly: 3.012 ± 0.05	0.621ProHis: 0.621 ± 0.021	2.277ProIle: 2.277 ± 0.04	2.2ProLys: 2.2 ± 0.037	3.494ProLeu: 3.494 ± 0.048	0.769ProMet: 0.769 ± 0.024	1.864ProAsn: 1.864 ± 0.035	0.986ProPro: 0.986 ± 0.032	1.356ProGln: 1.356 ± 0.027	1.106ProArg: 1.106 ± 0.026	2.356ProSer: 2.356 ± 0.044	1.817ProThr: 1.817 ± 0.048	3.345ProVal: 3.345 ± 0.048	0.4ProTrp: 0.4 ± 0.015	1.559ProTyr: 1.559 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	2.538GlnAla: 2.538 ± 0.039	0.175GlnCys: 0.175 ± 0.009	1.66GlnAsp: 1.66 ± 0.031	2.368GlnGlu: 2.368 ± 0.038	1.648GlnPhe: 1.648 ± 0.032	2.11GlnGly: 2.11 ± 0.036	0.786GlnHis: 0.786 ± 0.023	2.521GlnIle: 2.521 ± 0.033	2.696GlnLys: 2.696 ± 0.038	4.125GlnLeu: 4.125 ± 0.059	0.889GlnMet: 0.889 ± 0.021	2.048GlnAsn: 2.048 ± 0.034	1.384GlnPro: 1.384 ± 0.029	1.949GlnGln: 1.949 ± 0.036	1.513GlnArg: 1.513 ± 0.032	2.287GlnSer: 2.287 ± 0.033	2.018GlnThr: 2.018 ± 0.035	2.424GlnVal: 2.424 ± 0.039	0.446GlnTrp: 0.446 ± 0.025	1.512GlnTyr: 1.512 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.393ArgAla: 2.393 ± 0.035	0.24ArgCys: 0.24 ± 0.012	1.833ArgAsp: 1.833 ± 0.033	2.227ArgGlu: 2.227 ± 0.039	2.183ArgPhe: 2.183 ± 0.03	2.184ArgGly: 2.184 ± 0.035	0.65ArgHis: 0.65 ± 0.019	3.243ArgIle: 3.243 ± 0.045	3.022ArgLys: 3.022 ± 0.042	4.04ArgLeu: 4.04 ± 0.05	1.091ArgMet: 1.091 ± 0.025	2.386ArgAsn: 2.386 ± 0.039	1.383ArgPro: 1.383 ± 0.027	1.413ArgGln: 1.413 ± 0.028	1.536ArgArg: 1.536 ± 0.031	2.411ArgSer: 2.411 ± 0.038	2.148ArgThr: 2.148 ± 0.032	2.282ArgVal: 2.282 ± 0.036	0.526ArgTrp: 0.526 ± 0.018	1.869ArgTyr: 1.869 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.985SerAla: 4.985 ± 0.051	0.596SerCys: 0.596 ± 0.021	3.269SerAsp: 3.269 ± 0.044	3.195SerGlu: 3.195 ± 0.045	3.856SerPhe: 3.856 ± 0.051	5.545SerGly: 5.545 ± 0.081	1.043SerHis: 1.043 ± 0.026	4.83SerIle: 4.83 ± 0.056	4.235SerLys: 4.235 ± 0.05	6.684SerLeu: 6.684 ± 0.062	1.389SerMet: 1.389 ± 0.03	3.446SerAsn: 3.446 ± 0.059	2.619SerPro: 2.619 ± 0.041	1.974SerGln: 1.974 ± 0.032	2.645SerArg: 2.645 ± 0.041	4.654SerSer: 4.654 ± 0.067	3.7SerThr: 3.7 ± 0.067	4.258SerVal: 4.258 ± 0.045	0.927SerTrp: 0.927 ± 0.025	2.966SerTyr: 2.966 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	4.825ThrAla: 4.825 ± 0.125	0.311ThrCys: 0.311 ± 0.014	3.305ThrAsp: 3.305 ± 0.045	3.387ThrGlu: 3.387 ± 0.049	2.745ThrPhe: 2.745 ± 0.052	4.971ThrGly: 4.971 ± 0.12	0.893ThrHis: 0.893 ± 0.025	4.051ThrIle: 4.051 ± 0.068	3.187ThrLys: 3.187 ± 0.046	5.497ThrLeu: 5.497 ± 0.084	0.978ThrMet: 0.978 ± 0.023	2.777ThrAsn: 2.777 ± 0.054	2.454ThrPro: 2.454 ± 0.05	1.746ThrGln: 1.746 ± 0.032	1.893ThrArg: 1.893 ± 0.03	3.525ThrSer: 3.525 ± 0.062	3.334ThrThr: 3.334 ± 0.106	3.826ThrVal: 3.826 ± 0.086	0.611ThrTrp: 0.611 ± 0.02	2.431ThrTyr: 2.431 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	4.381ValAla: 4.381 ± 0.053	0.497ValCys: 0.497 ± 0.018	3.114ValAsp: 3.114 ± 0.043	3.316ValGlu: 3.316 ± 0.038	3.149ValPhe: 3.149 ± 0.042	3.516ValGly: 3.516 ± 0.051	0.983ValHis: 0.983 ± 0.023	4.634ValIle: 4.634 ± 0.052	4.552ValLys: 4.552 ± 0.053	6.201ValLeu: 6.201 ± 0.07	1.435ValMet: 1.435 ± 0.034	3.71ValAsn: 3.71 ± 0.065	2.506ValPro: 2.506 ± 0.035	2.049ValGln: 2.049 ± 0.036	2.216ValArg: 2.216 ± 0.034	4.552ValSer: 4.552 ± 0.057	3.639ValThr: 3.639 ± 0.102	4.037ValVal: 4.037 ± 0.052	0.658ValTrp: 0.658 ± 0.022	2.445ValTyr: 2.445 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	0.793TrpAla: 0.793 ± 0.023	0.12TrpCys: 0.12 ± 0.008	0.622TrpAsp: 0.622 ± 0.018	0.696TrpGlu: 0.696 ± 0.016	0.564TrpPhe: 0.564 ± 0.018	0.825TrpGly: 0.825 ± 0.024	0.224TrpHis: 0.224 ± 0.011	0.783TrpIle: 0.783 ± 0.023	0.906TrpLys: 0.906 ± 0.027	1.132TrpLeu: 1.132 ± 0.024	0.35TrpMet: 0.35 ± 0.014	0.734TrpAsn: 0.734 ± 0.021	0.351TrpPro: 0.351 ± 0.014	0.49TrpGln: 0.49 ± 0.015	0.456TrpArg: 0.456 ± 0.015	0.741TrpSer: 0.741 ± 0.022	0.667TrpThr: 0.667 ± 0.027	0.624TrpVal: 0.624 ± 0.021	0.203TrpTrp: 0.203 ± 0.011	0.519TrpTyr: 0.519 ± 0.031	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.838TyrAla: 2.838 ± 0.045	0.31TyrCys: 0.31 ± 0.012	2.239TyrAsp: 2.239 ± 0.049	2.075TyrGlu: 2.075 ± 0.033	2.287TyrPhe: 2.287 ± 0.039	2.867TyrGly: 2.867 ± 0.044	0.909TyrHis: 0.909 ± 0.023	2.464TyrIle: 2.464 ± 0.041	2.76TyrLys: 2.76 ± 0.058	4.159TyrLeu: 4.159 ± 0.051	0.761TyrMet: 0.761 ± 0.022	2.372TyrAsn: 2.372 ± 0.042	1.712TyrPro: 1.712 ± 0.032	1.877TyrGln: 1.877 ± 0.035	1.885TyrArg: 1.885 ± 0.036	2.684TyrSer: 2.684 ± 0.04	2.565TyrThr: 2.565 ± 0.046	2.165TyrVal: 2.165 ± 0.04	0.534TyrTrp: 0.534 ± 0.019	1.861TyrTyr: 1.861 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5356 proteins (1988063 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---