American plum line pattern virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q997A3|Q997A3_9BROM RNA-directed RNA polymerase 2a OS=American plum line pattern virus OX=134632 PE=4 SV=1
MM1 pKa = 7.51AKK3 pKa = 9.77EE4 pKa = 4.15SSSGFSTRR12 pKa = 11.84VTSRR16 pKa = 11.84LDD18 pKa = 3.75AIAEE22 pKa = 3.97EE23 pKa = 4.72RR24 pKa = 11.84EE25 pKa = 3.76MDD27 pKa = 3.58EE28 pKa = 5.3FLDD31 pKa = 4.44FVTKK35 pKa = 10.75SPIKK39 pKa = 10.6QKK41 pKa = 9.38MCCDD45 pKa = 3.34IPLKK49 pKa = 10.34NSEE52 pKa = 4.21MLSFVNLVTKK62 pKa = 10.24EE63 pKa = 4.28SKK65 pKa = 10.77SFLSLFKK72 pKa = 10.63KK73 pKa = 10.08IPVYY77 pKa = 10.22TNHH80 pKa = 7.14PLISVIYY87 pKa = 9.81IPVVLNDD94 pKa = 3.77APGVLDD100 pKa = 3.58VALVNPITRR109 pKa = 11.84DD110 pKa = 3.16RR111 pKa = 11.84RR112 pKa = 11.84EE113 pKa = 4.03ILCNWPLSKK122 pKa = 10.72AVLVMTNWTRR132 pKa = 11.84SIAKK136 pKa = 9.78GAEE139 pKa = 3.78LNLAYY144 pKa = 9.7EE145 pKa = 4.39VSNTQVKK152 pKa = 10.35LDD154 pKa = 3.61QQIGRR159 pKa = 11.84FRR161 pKa = 11.84ILWADD166 pKa = 3.58VPSSKK171 pKa = 10.4QIYY174 pKa = 8.5EE175 pKa = 4.07KK176 pKa = 10.27EE177 pKa = 4.16RR178 pKa = 11.84PPMIVDD184 pKa = 4.39LEE186 pKa = 4.39EE187 pKa = 4.47TGCTMHH193 pKa = 6.75QYY195 pKa = 11.21SDD197 pKa = 3.35QTLLQFVNATMVSGVRR213 pKa = 11.84KK214 pKa = 9.05PRR216 pKa = 11.84EE217 pKa = 3.75DD218 pKa = 3.45RR219 pKa = 11.84KK220 pKa = 10.64LLRR223 pKa = 11.84LKK225 pKa = 10.32SLRR228 pKa = 11.84TGFEE232 pKa = 3.76IEE234 pKa = 4.08PAQDD238 pKa = 3.47DD239 pKa = 3.97EE240 pKa = 5.8ADD242 pKa = 3.67EE243 pKa = 4.66GKK245 pKa = 10.1KK246 pKa = 10.92GPIIVPSAPEE256 pKa = 3.62EE257 pKa = 3.73LHH259 pKa = 6.46RR260 pKa = 11.84PFVIEE265 pKa = 3.99GEE267 pKa = 4.45GVIGGPIMTVIEE279 pKa = 4.37FEE281 pKa = 5.16DD282 pKa = 4.11IAWHH286 pKa = 7.17LGDD289 pKa = 3.72QQ290 pKa = 3.8

Molecular weight: 32.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q997A2|Q997A2_9BROM Movement protein OS=American plum line pattern virus OX=134632 PE=4 SV=1
MM1 pKa = 7.64LKK3 pKa = 9.89MNAPQNKK10 pKa = 9.01GKK12 pKa = 10.44KK13 pKa = 8.24KK14 pKa = 9.72QNARR18 pKa = 11.84TTQFAQRR25 pKa = 11.84RR26 pKa = 11.84AAAARR31 pKa = 11.84GEE33 pKa = 4.31IEE35 pKa = 4.05TSSRR39 pKa = 11.84NITARR44 pKa = 11.84SGGPPVPRR52 pKa = 11.84SIRR55 pKa = 11.84TEE57 pKa = 3.77WEE59 pKa = 3.7LVGPSVGARR68 pKa = 11.84LIQGPHH74 pKa = 5.4NEE76 pKa = 4.26VVKK79 pKa = 11.07GEE81 pKa = 4.11AVASSTTAGQYY92 pKa = 10.45FSIQISTYY100 pKa = 9.57LEE102 pKa = 4.14KK103 pKa = 10.41FLEE106 pKa = 4.35MGVQLNSMTVLCASLQGSGIVCLTHH131 pKa = 7.59KK132 pKa = 9.31FTTCEE137 pKa = 3.7AAISTLSGLRR147 pKa = 11.84FRR149 pKa = 11.84EE150 pKa = 4.14GRR152 pKa = 11.84RR153 pKa = 11.84LGFQVLPPSDD163 pKa = 3.47TVAGDD168 pKa = 3.23VASNVRR174 pKa = 11.84LVFKK178 pKa = 10.48FDD180 pKa = 3.44KK181 pKa = 10.85AFEE184 pKa = 4.52ANAPLIEE191 pKa = 4.16RR192 pKa = 11.84KK193 pKa = 9.74VWVSTSRR200 pKa = 11.84LPEE203 pKa = 3.46IMIPANLLVPDD214 pKa = 4.32EE215 pKa = 4.57DD216 pKa = 3.82

Molecular weight: 23.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2308

216

1062

577.0

65.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.023 ± 0.767

2.21 ± 0.302

6.066 ± 0.706

6.586 ± 0.624

5.199 ± 0.603

5.069 ± 0.401

2.21 ± 0.355

5.156 ± 0.735

5.936 ± 0.128

8.925 ± 0.835

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.08 ± 0.266

3.423 ± 0.478

4.809 ± 0.487

2.946 ± 0.334

6.326 ± 0.417

8.362 ± 0.357

5.719 ± 0.158

8.536 ± 0.196

1.213 ± 0.177

3.206 ± 0.606
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski