Salmonella phage 64795_sal3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173GCU5|A0A173GCU5_9CAUD Uncharacterized protein OS=Salmonella phage 64795_sal3 OX=1813769 PE=4 SV=1
MM1 pKa = 7.84IPLLWILSAYY11 pKa = 10.12AFARR15 pKa = 11.84VFEE18 pKa = 4.69ADD20 pKa = 3.27SLYY23 pKa = 11.53QMICYY28 pKa = 9.15GALFCLSGAALAFMDD43 pKa = 5.54DD44 pKa = 4.37VISDD48 pKa = 3.56

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173GBX5|A0A173GBX5_9CAUD Uncharacterized protein OS=Salmonella phage 64795_sal3 OX=1813769 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84KK3 pKa = 9.85FSTLVDD9 pKa = 5.31LILAEE14 pKa = 4.24EE15 pKa = 4.32SNARR19 pKa = 11.84NKK21 pKa = 10.24KK22 pKa = 9.85SRR24 pKa = 11.84NQRR27 pKa = 11.84QDD29 pKa = 2.32ITFRR33 pKa = 11.84TLLKK37 pKa = 10.41QSMRR41 pKa = 11.84GKK43 pKa = 10.36KK44 pKa = 6.63EE45 pKa = 3.06WRR47 pKa = 11.84YY48 pKa = 10.11RR49 pKa = 11.84RR50 pKa = 11.84DD51 pKa = 3.04RR52 pKa = 11.84VLIKK56 pKa = 10.53LCKK59 pKa = 10.32LNMNKK64 pKa = 8.07TLKK67 pKa = 11.0DD68 pKa = 3.23MTKK71 pKa = 10.71

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13426

31

828

181.4

20.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.61 ± 0.564

1.601 ± 0.2

6.316 ± 0.291

6.517 ± 0.314

3.329 ± 0.177

7.15 ± 0.298

1.84 ± 0.199

6.636 ± 0.186

7.217 ± 0.389

6.927 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.233 ± 0.184

4.797 ± 0.202

3.143 ± 0.238

3.881 ± 0.308

5.08 ± 0.232

6.428 ± 0.294

5.4 ± 0.388

6.547 ± 0.257

1.564 ± 0.15

3.784 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski