Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Phaffomycetaceae; Komagataella; Komagataella phaffii

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5073 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4QWT3|C4QWT3_KOMPG MICOS complex subunit MIC10 OS=Komagataella phaffii (strain GS115 / ATCC 20864) OX=644223 GN=PAS_chr1-1_0330 PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84PVLSLLLLLASSVLADD18 pKa = 4.04EE19 pKa = 5.8VIEE22 pKa = 4.41CDD24 pKa = 4.71ADD26 pKa = 3.85NKK28 pKa = 10.72CPEE31 pKa = 4.95DD32 pKa = 4.31KK33 pKa = 10.34PCCSQYY39 pKa = 10.84GVCGTGVNCLGGCDD53 pKa = 3.84PRR55 pKa = 11.84HH56 pKa = 6.08SFNASACLPMPVCRR70 pKa = 11.84DD71 pKa = 3.06VDD73 pKa = 4.42LKK75 pKa = 11.46ASTDD79 pKa = 3.21AFEE82 pKa = 5.9IDD84 pKa = 3.38TNYY87 pKa = 10.9LGDD90 pKa = 4.05ANEE93 pKa = 4.28TDD95 pKa = 3.28WVYY98 pKa = 11.44NGYY101 pKa = 10.47LIDD104 pKa = 4.35YY105 pKa = 9.32DD106 pKa = 5.01DD107 pKa = 4.52SVLLAMPKK115 pKa = 9.77EE116 pKa = 4.5SYY118 pKa = 9.18GTVVSSTFYY127 pKa = 10.15VWYY130 pKa = 10.08GKK132 pKa = 8.1ITATLKK138 pKa = 8.57TSRR141 pKa = 11.84GAGVVTSFILFSNVHH156 pKa = 6.91DD157 pKa = 5.0EE158 pKa = 4.19IDD160 pKa = 3.59WEE162 pKa = 4.36FVGYY166 pKa = 10.18NLSQVEE172 pKa = 4.29TNYY175 pKa = 10.72YY176 pKa = 8.05YY177 pKa = 10.54QGVLNYY183 pKa = 9.97TNGRR187 pKa = 11.84NVSLEE192 pKa = 3.69EE193 pKa = 4.02DD194 pKa = 3.86VNSFEE199 pKa = 4.33YY200 pKa = 10.41FHH202 pKa = 8.04DD203 pKa = 4.56YY204 pKa = 10.77EE205 pKa = 5.6IDD207 pKa = 3.26WKK209 pKa = 10.87EE210 pKa = 4.64DD211 pKa = 3.41VITWSIDD218 pKa = 2.88GDD220 pKa = 3.95VVRR223 pKa = 11.84TLKK226 pKa = 10.95KK227 pKa = 9.81EE228 pKa = 3.72DD229 pKa = 3.82TYY231 pKa = 11.96NEE233 pKa = 4.18TTDD236 pKa = 4.49KK237 pKa = 11.47YY238 pKa = 10.19MFPQTPSRR246 pKa = 11.84VQLSIWPAGAEE257 pKa = 4.13SNAIGTVSWAGGNVDD272 pKa = 4.36WDD274 pKa = 4.0SEE276 pKa = 4.55DD277 pKa = 3.48IQDD280 pKa = 4.11PGYY283 pKa = 10.54FYY285 pKa = 10.88YY286 pKa = 10.13TLKK289 pKa = 10.62EE290 pKa = 4.14LTVEE294 pKa = 4.62CYY296 pKa = 10.07DD297 pKa = 3.73VPDD300 pKa = 3.82GTEE303 pKa = 3.71EE304 pKa = 4.26DD305 pKa = 4.41GEE307 pKa = 3.97LAYY310 pKa = 10.75YY311 pKa = 10.35FKK313 pKa = 11.03EE314 pKa = 4.06SDD316 pKa = 4.03AFDD319 pKa = 3.34QGDD322 pKa = 3.8IIITNNSTKK331 pKa = 10.16IKK333 pKa = 10.71SLDD336 pKa = 3.52DD337 pKa = 3.33TGFDD341 pKa = 4.43PDD343 pKa = 4.8EE344 pKa = 6.41DD345 pKa = 5.63DD346 pKa = 6.5DD347 pKa = 6.26DD348 pKa = 6.44DD349 pKa = 4.85EE350 pKa = 5.94SSSSSSSSSRR360 pKa = 11.84SSSSSSRR367 pKa = 11.84TGSSSTSSATSTSTSNSNDD386 pKa = 3.3DD387 pKa = 4.24DD388 pKa = 5.07DD389 pKa = 5.66NNNSSPTTSSGTSSAASGFVQNMSQTSGSSSATSNNAAASLSAGFLTTISFFASVLGFLL448 pKa = 4.84

Molecular weight: 48.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4R3E9|C4R3E9_KOMPG Mitochondrial intermembrane space cysteine motif protein OS=Komagataella phaffii (strain GS115 / ATCC 20864) OX=644223 GN=PAS_chr3_0056 PE=3 SV=1
MM1 pKa = 7.27PCHH4 pKa = 6.55RR5 pKa = 11.84RR6 pKa = 11.84MMDD9 pKa = 2.5IRR11 pKa = 11.84KK12 pKa = 9.4VIKK15 pKa = 10.31FSDD18 pKa = 3.44VGDD21 pKa = 3.2ITMAVCGIHH30 pKa = 5.83FRR32 pKa = 11.84CRR34 pKa = 11.84LFSFNKK40 pKa = 9.42GIRR43 pKa = 11.84RR44 pKa = 11.84QSYY47 pKa = 7.78STII50 pKa = 3.5

Molecular weight: 5.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5073

0

5073

2416083

45

4950

476.3

53.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.514 ± 0.027

1.17 ± 0.013

5.917 ± 0.024

6.786 ± 0.037

4.463 ± 0.023

5.127 ± 0.031

2.13 ± 0.014

6.34 ± 0.025

6.944 ± 0.03

10.131 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.853 ± 0.012

5.34 ± 0.023

4.489 ± 0.025

4.193 ± 0.029

4.554 ± 0.019

8.916 ± 0.041

5.69 ± 0.028

6.069 ± 0.023

1.035 ± 0.011

3.333 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski