Prevotella sp. CAG:1058

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; environmental samples

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5C735|R5C735_9BACT Uncharacterized protein OS=Prevotella sp. CAG:1058 OX=1262918 GN=BN458_01097 PE=4 SV=1
MM1 pKa = 8.09LMFFATTAVSCGNDD15 pKa = 3.55DD16 pKa = 3.64YY17 pKa = 12.05DD18 pKa = 5.15SYY20 pKa = 11.42TGQNVVNGTDD30 pKa = 3.24NGEE33 pKa = 4.24SEE35 pKa = 4.6NDD37 pKa = 3.2STQQSNNNGQNEE49 pKa = 4.27EE50 pKa = 4.04EE51 pKa = 4.31MTRR54 pKa = 11.84KK55 pKa = 8.3ITISAGGANFTATLADD71 pKa = 3.46NRR73 pKa = 11.84TAQAFASMLPLTLDD87 pKa = 3.41MNEE90 pKa = 4.05LNGNEE95 pKa = 4.13KK96 pKa = 10.61YY97 pKa = 10.52FYY99 pKa = 10.63LDD101 pKa = 2.72SSLPADD107 pKa = 3.89SNNPGTINAGDD118 pKa = 3.36IMLYY122 pKa = 10.79GSDD125 pKa = 4.37CLVLFYY131 pKa = 10.29KK132 pKa = 10.19TFSTSYY138 pKa = 8.66SYY140 pKa = 11.2TRR142 pKa = 11.84IGSVDD147 pKa = 3.43NPQQLAEE154 pKa = 3.97ALGRR158 pKa = 11.84GNVKK162 pKa = 8.76VTFSAAEE169 pKa = 3.74

Molecular weight: 18.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5CTH2|R5CTH2_9BACT Uncharacterized protein OS=Prevotella sp. CAG:1058 OX=1262918 GN=BN458_00410 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2263

0

2263

756260

29

1575

334.2

37.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.713 ± 0.059

1.424 ± 0.021

6.074 ± 0.043

5.995 ± 0.053

4.317 ± 0.038

7.29 ± 0.05

1.83 ± 0.025

6.7 ± 0.05

6.42 ± 0.054

8.581 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.046 ± 0.026

5.051 ± 0.044

3.596 ± 0.026

2.997 ± 0.022

4.877 ± 0.051

5.947 ± 0.039

5.689 ± 0.044

6.974 ± 0.048

1.159 ± 0.021

4.318 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski