Amino acid dipepetide frequency for Oscillatoriales cyanobacterium USR001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.03AlaAla: 7.03 ± 0.09	0.755AlaCys: 0.755 ± 0.019	3.944AlaAsp: 3.944 ± 0.058	5.539AlaGlu: 5.539 ± 0.075	2.778AlaPhe: 2.778 ± 0.046	5.196AlaGly: 5.196 ± 0.078	1.1AlaHis: 1.1 ± 0.031	7.771AlaIle: 7.771 ± 0.088	4.543AlaLys: 4.543 ± 0.057	7.854AlaLeu: 7.854 ± 0.088	1.559AlaMet: 1.559 ± 0.031	3.887AlaAsn: 3.887 ± 0.074	2.615AlaPro: 2.615 ± 0.055	3.629AlaGln: 3.629 ± 0.06	3.261AlaArg: 3.261 ± 0.043	4.366AlaSer: 4.366 ± 0.063	4.538AlaThr: 4.538 ± 0.075	4.971AlaVal: 4.971 ± 0.067	0.896AlaTrp: 0.896 ± 0.022	2.162AlaTyr: 2.162 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.607CysAla: 0.607 ± 0.024	0.166CysCys: 0.166 ± 0.011	0.627CysAsp: 0.627 ± 0.021	0.542CysGlu: 0.542 ± 0.018	0.419CysPhe: 0.419 ± 0.016	0.74CysGly: 0.74 ± 0.021	0.266CysHis: 0.266 ± 0.015	0.576CysIle: 0.576 ± 0.018	0.367CysLys: 0.367 ± 0.014	1.138CysLeu: 1.138 ± 0.03	0.158CysMet: 0.158 ± 0.011	0.377CysAsn: 0.377 ± 0.016	0.495CysPro: 0.495 ± 0.018	0.646CysGln: 0.646 ± 0.022	0.492CysArg: 0.492 ± 0.019	0.544CysSer: 0.544 ± 0.018	0.434CysThr: 0.434 ± 0.019	0.535CysVal: 0.535 ± 0.021	0.138CysTrp: 0.138 ± 0.01	0.426CysTyr: 0.426 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.512AspAla: 3.512 ± 0.05	0.523AspCys: 0.523 ± 0.018	2.318AspAsp: 2.318 ± 0.041	3.044AspGlu: 3.044 ± 0.052	2.503AspPhe: 2.503 ± 0.047	3.429AspGly: 3.429 ± 0.063	0.568AspHis: 0.568 ± 0.022	3.619AspIle: 3.619 ± 0.059	2.269AspLys: 2.269 ± 0.045	5.859AspLeu: 5.859 ± 0.075	0.832AspMet: 0.832 ± 0.023	2.049AspAsn: 2.049 ± 0.035	2.265AspPro: 2.265 ± 0.045	1.401AspGln: 1.401 ± 0.03	4.41AspArg: 4.41 ± 0.057	3.003AspSer: 3.003 ± 0.05	2.372AspThr: 2.372 ± 0.049	2.859AspVal: 2.859 ± 0.043	0.901AspTrp: 0.901 ± 0.022	1.851AspTyr: 1.851 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	5.409GluAla: 5.409 ± 0.068	0.522GluCys: 0.522 ± 0.018	2.758GluAsp: 2.758 ± 0.05	4.692GluGlu: 4.692 ± 0.074	2.637GluPhe: 2.637 ± 0.037	3.641GluGly: 3.641 ± 0.061	0.91GluHis: 0.91 ± 0.025	5.912GluIle: 5.912 ± 0.064	4.317GluLys: 4.317 ± 0.059	7.671GluLeu: 7.671 ± 0.091	1.573GluMet: 1.573 ± 0.03	3.308GluAsn: 3.308 ± 0.054	2.382GluPro: 2.382 ± 0.042	3.318GluGln: 3.318 ± 0.064	3.304GluArg: 3.304 ± 0.059	3.871GluSer: 3.871 ± 0.059	3.712GluThr: 3.712 ± 0.05	4.401GluVal: 4.401 ± 0.057	0.816GluTrp: 0.816 ± 0.024	1.933GluTyr: 1.933 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	2.959PheAla: 2.959 ± 0.044	0.535PheCys: 0.535 ± 0.018	2.289PheAsp: 2.289 ± 0.043	2.275PheGlu: 2.275 ± 0.041	1.704PhePhe: 1.704 ± 0.036	2.853PheGly: 2.853 ± 0.051	0.7PheHis: 0.7 ± 0.023	2.453PheIle: 2.453 ± 0.038	1.685PheLys: 1.685 ± 0.036	4.235PheLeu: 4.235 ± 0.06	0.693PheMet: 0.693 ± 0.019	1.939PheAsn: 1.939 ± 0.04	1.954PhePro: 1.954 ± 0.037	1.84PheGln: 1.84 ± 0.037	1.767PheArg: 1.767 ± 0.038	2.987PheSer: 2.987 ± 0.056	2.365PheThr: 2.365 ± 0.039	2.348PheVal: 2.348 ± 0.038	0.691PheTrp: 0.691 ± 0.021	1.418PheTyr: 1.418 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	4.682GlyAla: 4.682 ± 0.083	0.738GlyCys: 0.738 ± 0.022	3.569GlyAsp: 3.569 ± 0.071	4.363GlyGlu: 4.363 ± 0.057	2.882GlyPhe: 2.882 ± 0.041	4.894GlyGly: 4.894 ± 0.126	1.048GlyHis: 1.048 ± 0.03	5.367GlyIle: 5.367 ± 0.065	4.671GlyLys: 4.671 ± 0.06	6.657GlyLeu: 6.657 ± 0.075	1.496GlyMet: 1.496 ± 0.034	3.719GlyAsn: 3.719 ± 0.11	1.363GlyPro: 1.363 ± 0.035	2.765GlyGln: 2.765 ± 0.046	3.114GlyArg: 3.114 ± 0.058	3.995GlySer: 3.995 ± 0.072	3.756GlyThr: 3.756 ± 0.083	4.337GlyVal: 4.337 ± 0.055	1.048GlyTrp: 1.048 ± 0.029	2.252GlyTyr: 2.252 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	0.898HisAla: 0.898 ± 0.022	0.236HisCys: 0.236 ± 0.013	0.608HisAsp: 0.608 ± 0.021	0.793HisGlu: 0.793 ± 0.022	0.75HisPhe: 0.75 ± 0.023	0.932HisGly: 0.932 ± 0.023	0.509HisHis: 0.509 ± 0.023	1.013HisIle: 1.013 ± 0.024	0.735HisLys: 0.735 ± 0.023	2.11HisLeu: 2.11 ± 0.037	0.131HisMet: 0.131 ± 0.009	0.717HisAsn: 0.717 ± 0.021	1.24HisPro: 1.24 ± 0.032	1.035HisGln: 1.035 ± 0.029	0.968HisArg: 0.968 ± 0.026	1.102HisSer: 1.102 ± 0.025	0.742HisThr: 0.742 ± 0.021	0.659HisVal: 0.659 ± 0.02	0.299HisTrp: 0.299 ± 0.013	0.615HisTyr: 0.615 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.677IleAla: 7.677 ± 0.077	0.833IleCys: 0.833 ± 0.024	4.04IleAsp: 4.04 ± 0.049	5.124IleGlu: 5.124 ± 0.066	2.885IlePhe: 2.885 ± 0.049	4.838IleGly: 4.838 ± 0.064	1.191IleHis: 1.191 ± 0.029	4.468IleIle: 4.468 ± 0.066	3.634IleLys: 3.634 ± 0.051	7.399IleLeu: 7.399 ± 0.082	0.957IleMet: 0.957 ± 0.026	3.485IleAsn: 3.485 ± 0.054	3.77IlePro: 3.77 ± 0.049	3.005IleGln: 3.005 ± 0.046	3.178IleArg: 3.178 ± 0.046	5.039IleSer: 5.039 ± 0.059	3.929IleThr: 3.929 ± 0.062	4.579IleVal: 4.579 ± 0.062	0.97IleTrp: 0.97 ± 0.023	2.198IleTyr: 2.198 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.135LysAla: 4.135 ± 0.056	0.35LysCys: 0.35 ± 0.015	2.443LysAsp: 2.443 ± 0.04	3.348LysGlu: 3.348 ± 0.056	2.096LysPhe: 2.096 ± 0.038	2.946LysGly: 2.946 ± 0.046	0.805LysHis: 0.805 ± 0.024	4.32LysIle: 4.32 ± 0.052	2.699LysLys: 2.699 ± 0.056	5.807LysLeu: 5.807 ± 0.068	1.142LysMet: 1.142 ± 0.025	2.501LysAsn: 2.501 ± 0.042	2.57LysPro: 2.57 ± 0.047	2.627LysGln: 2.627 ± 0.046	2.412LysArg: 2.412 ± 0.043	3.477LysSer: 3.477 ± 0.049	3.032LysThr: 3.032 ± 0.048	3.248LysVal: 3.248 ± 0.053	0.565LysTrp: 0.565 ± 0.021	1.545LysTyr: 1.545 ± 0.032	0.0LysXaa: 0.0 ± 0.0
Leu	9.354LeuAla: 9.354 ± 0.101	0.949LeuCys: 0.949 ± 0.024	5.349LeuAsp: 5.349 ± 0.06	7.658LeuGlu: 7.658 ± 0.087	3.661LeuPhe: 3.661 ± 0.061	7.62LeuGly: 7.62 ± 0.073	1.61LeuHis: 1.61 ± 0.036	7.355LeuIle: 7.355 ± 0.077	5.852LeuLys: 5.852 ± 0.068	10.921LeuLeu: 10.921 ± 0.118	2.219LeuMet: 2.219 ± 0.041	4.942LeuAsn: 4.942 ± 0.064	5.635LeuPro: 5.635 ± 0.071	5.302LeuGln: 5.302 ± 0.076	5.483LeuArg: 5.483 ± 0.069	7.651LeuSer: 7.651 ± 0.08	6.562LeuThr: 6.562 ± 0.08	6.709LeuVal: 6.709 ± 0.065	1.424LeuTrp: 1.424 ± 0.035	2.623LeuTyr: 2.623 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.741MetAla: 1.741 ± 0.031	0.117MetCys: 0.117 ± 0.009	0.752MetAsp: 0.752 ± 0.025	1.091MetGlu: 1.091 ± 0.028	0.569MetPhe: 0.569 ± 0.019	1.409MetGly: 1.409 ± 0.034	0.207MetHis: 0.207 ± 0.012	1.193MetIle: 1.193 ± 0.031	1.062MetLys: 1.062 ± 0.026	1.908MetLeu: 1.908 ± 0.033	0.457MetMet: 0.457 ± 0.016	0.884MetAsn: 0.884 ± 0.023	1.015MetPro: 1.015 ± 0.026	0.91MetGln: 0.91 ± 0.026	0.983MetArg: 0.983 ± 0.026	1.366MetSer: 1.366 ± 0.031	1.346MetThr: 1.346 ± 0.03	1.308MetVal: 1.308 ± 0.032	0.173MetTrp: 0.173 ± 0.011	0.346MetTyr: 0.346 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.134AsnAla: 3.134 ± 0.057	0.546AsnCys: 0.546 ± 0.022	2.124AsnAsp: 2.124 ± 0.056	2.145AsnGlu: 2.145 ± 0.036	2.199AsnPhe: 2.199 ± 0.04	2.975AsnGly: 2.975 ± 0.071	0.832AsnHis: 0.832 ± 0.022	3.231AsnIle: 3.231 ± 0.061	1.832AsnLys: 1.832 ± 0.036	6.284AsnLeu: 6.284 ± 0.102	0.744AsnMet: 0.744 ± 0.021	2.413AsnAsn: 2.413 ± 0.054	3.045AsnPro: 3.045 ± 0.056	3.007AsnGln: 3.007 ± 0.054	2.471AsnArg: 2.471 ± 0.043	3.594AsnSer: 3.594 ± 0.064	2.279AsnThr: 2.279 ± 0.048	2.278AsnVal: 2.278 ± 0.045	0.83AsnTrp: 0.83 ± 0.026	1.716AsnTyr: 1.716 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	3.125ProAla: 3.125 ± 0.068	0.355ProCys: 0.355 ± 0.017	2.954ProAsp: 2.954 ± 0.047	4.05ProGlu: 4.05 ± 0.052	1.706ProPhe: 1.706 ± 0.036	2.878ProGly: 2.878 ± 0.055	0.812ProHis: 0.812 ± 0.026	3.354ProIle: 3.354 ± 0.049	2.334ProLys: 2.334 ± 0.047	4.539ProLeu: 4.539 ± 0.058	0.763ProMet: 0.763 ± 0.023	2.461ProAsn: 2.461 ± 0.05	2.345ProPro: 2.345 ± 0.051	2.35ProGln: 2.35 ± 0.036	1.659ProArg: 1.659 ± 0.032	2.823ProSer: 2.823 ± 0.051	3.068ProThr: 3.068 ± 0.086	3.106ProVal: 3.106 ± 0.05	0.553ProTrp: 0.553 ± 0.018	1.221ProTyr: 1.221 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	4.108GlnAla: 4.108 ± 0.068	0.338GlnCys: 0.338 ± 0.018	1.926GlnAsp: 1.926 ± 0.031	3.607GlnGlu: 3.607 ± 0.062	1.813GlnPhe: 1.813 ± 0.036	2.987GlnGly: 2.987 ± 0.047	0.747GlnHis: 0.747 ± 0.021	3.88GlnIle: 3.88 ± 0.049	2.946GlnLys: 2.946 ± 0.049	5.807GlnLeu: 5.807 ± 0.076	0.958GlnMet: 0.958 ± 0.025	2.29GlnAsn: 2.29 ± 0.039	2.299GlnPro: 2.299 ± 0.044	3.266GlnGln: 3.266 ± 0.086	2.495GlnArg: 2.495 ± 0.046	2.852GlnSer: 2.852 ± 0.049	2.712GlnThr: 2.712 ± 0.043	3.348GlnVal: 3.348 ± 0.05	0.616GlnTrp: 0.616 ± 0.02	1.151GlnTyr: 1.151 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	3.115ArgAla: 3.115 ± 0.049	0.494ArgCys: 0.494 ± 0.019	2.731ArgAsp: 2.731 ± 0.043	3.651ArgGlu: 3.651 ± 0.053	2.077ArgPhe: 2.077 ± 0.043	3.031ArgGly: 3.031 ± 0.053	0.937ArgHis: 0.937 ± 0.024	3.545ArgIle: 3.545 ± 0.049	2.356ArgLys: 2.356 ± 0.041	5.73ArgLeu: 5.73 ± 0.073	1.003ArgMet: 1.003 ± 0.025	2.204ArgAsn: 2.204 ± 0.038	1.904ArgPro: 1.904 ± 0.036	3.018ArgGln: 3.018 ± 0.051	2.945ArgArg: 2.945 ± 0.048	3.335ArgSer: 3.335 ± 0.048	2.35ArgThr: 2.35 ± 0.035	3.102ArgVal: 3.102 ± 0.052	0.757ArgTrp: 0.757 ± 0.024	1.771ArgTyr: 1.771 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	4.486SerAla: 4.486 ± 0.067	0.6SerCys: 0.6 ± 0.022	3.345SerAsp: 3.345 ± 0.048	4.24SerGlu: 4.24 ± 0.054	2.55SerPhe: 2.55 ± 0.045	4.828SerGly: 4.828 ± 0.072	1.205SerHis: 1.205 ± 0.029	4.112SerIle: 4.112 ± 0.061	2.841SerLys: 2.841 ± 0.045	7.404SerLeu: 7.404 ± 0.077	1.196SerMet: 1.196 ± 0.029	2.918SerAsn: 2.918 ± 0.05	3.524SerPro: 3.524 ± 0.052	3.679SerGln: 3.679 ± 0.053	3.089SerArg: 3.089 ± 0.045	4.392SerSer: 4.392 ± 0.066	3.303SerThr: 3.303 ± 0.059	3.904SerVal: 3.904 ± 0.052	0.897SerTrp: 0.897 ± 0.025	1.837SerTyr: 1.837 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	4.579ThrAla: 4.579 ± 0.07	0.445ThrCys: 0.445 ± 0.018	2.586ThrAsp: 2.586 ± 0.047	3.574ThrGlu: 3.574 ± 0.05	2.079ThrPhe: 2.079 ± 0.045	4.329ThrGly: 4.329 ± 0.077	0.891ThrHis: 0.891 ± 0.028	4.162ThrIle: 4.162 ± 0.067	2.438ThrLys: 2.438 ± 0.042	6.13ThrLeu: 6.13 ± 0.067	0.819ThrMet: 0.819 ± 0.023	2.596ThrAsn: 2.596 ± 0.056	3.47ThrPro: 3.47 ± 0.092	2.606ThrGln: 2.606 ± 0.047	2.214ThrArg: 2.214 ± 0.044	3.274ThrSer: 3.274 ± 0.07	3.241ThrThr: 3.241 ± 0.087	3.81ThrVal: 3.81 ± 0.067	0.67ThrTrp: 0.67 ± 0.022	1.521ThrTyr: 1.521 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	5.129ValAla: 5.129 ± 0.067	0.662ValCys: 0.662 ± 0.024	3.139ValAsp: 3.139 ± 0.049	4.572ValGlu: 4.572 ± 0.05	2.413ValPhe: 2.413 ± 0.038	4.297ValGly: 4.297 ± 0.071	0.818ValHis: 0.818 ± 0.02	4.268ValIle: 4.268 ± 0.056	3.637ValLys: 3.637 ± 0.055	6.018ValLeu: 6.018 ± 0.071	1.37ValMet: 1.37 ± 0.03	3.091ValAsn: 3.091 ± 0.051	2.59ValPro: 2.59 ± 0.05	2.527ValGln: 2.527 ± 0.042	3.011ValArg: 3.011 ± 0.046	4.043ValSer: 4.043 ± 0.056	3.757ValThr: 3.757 ± 0.05	4.194ValVal: 4.194 ± 0.059	0.737ValTrp: 0.737 ± 0.026	1.786ValTyr: 1.786 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.79TrpAla: 0.79 ± 0.019	0.137TrpCys: 0.137 ± 0.011	0.642TrpAsp: 0.642 ± 0.023	1.06TrpGlu: 1.06 ± 0.028	0.554TrpPhe: 0.554 ± 0.02	0.908TrpGly: 0.908 ± 0.027	0.291TrpHis: 0.291 ± 0.014	0.877TrpIle: 0.877 ± 0.026	0.61TrpLys: 0.61 ± 0.019	1.818TrpLeu: 1.818 ± 0.041	0.354TrpMet: 0.354 ± 0.015	0.615TrpAsn: 0.615 ± 0.024	0.4TrpPro: 0.4 ± 0.017	1.061TrpGln: 1.061 ± 0.028	0.815TrpArg: 0.815 ± 0.022	0.72TrpSer: 0.72 ± 0.024	0.512TrpThr: 0.512 ± 0.018	0.89TrpVal: 0.89 ± 0.024	0.197TrpTrp: 0.197 ± 0.01	0.412TrpTyr: 0.412 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.926TyrAla: 1.926 ± 0.035	0.391TyrCys: 0.391 ± 0.016	1.443TyrAsp: 1.443 ± 0.037	1.72TyrGlu: 1.72 ± 0.036	1.414TyrPhe: 1.414 ± 0.034	1.986TyrGly: 1.986 ± 0.037	0.647TyrHis: 0.647 ± 0.023	1.723TyrIle: 1.723 ± 0.033	1.237TyrLys: 1.237 ± 0.032	3.589TyrLeu: 3.589 ± 0.05	0.413TyrMet: 0.413 ± 0.015	1.281TyrAsn: 1.281 ± 0.033	1.577TyrPro: 1.577 ± 0.037	2.155TyrGln: 2.155 ± 0.04	1.987TyrArg: 1.987 ± 0.035	1.916TyrSer: 1.916 ± 0.041	1.423TyrThr: 1.423 ± 0.029	1.469TyrVal: 1.469 ± 0.029	0.487TyrTrp: 0.487 ± 0.02	1.134TyrTyr: 1.134 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4908 proteins (1600603 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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