Hubei noda-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KGX6|A0A1L3KGX6_9VIRU Capsid protein OS=Hubei noda-like virus 9 OX=1922989 PE=4 SV=1
MM1 pKa = 7.85ANNNNNAQNTQRR13 pKa = 11.84GNNSNQPMGTNKK25 pKa = 9.33VKK27 pKa = 10.36RR28 pKa = 11.84SKK30 pKa = 10.49RR31 pKa = 11.84RR32 pKa = 11.84SKK34 pKa = 11.23QNVNNNAKK42 pKa = 8.78PTYY45 pKa = 9.81NPPNQTRR52 pKa = 11.84SGRR55 pKa = 11.84MPNPADD61 pKa = 3.42PRR63 pKa = 11.84TAIRR67 pKa = 11.84NLGNTQLGSCAAMKK81 pKa = 10.09ILHH84 pKa = 6.87PCNEE88 pKa = 3.77FDD90 pKa = 4.2IEE92 pKa = 4.57VVKK95 pKa = 10.92IPDD98 pKa = 3.51GSLPTSVAFEE108 pKa = 3.96RR109 pKa = 11.84RR110 pKa = 11.84DD111 pKa = 3.53EE112 pKa = 4.21YY113 pKa = 10.94EE114 pKa = 3.45IRR116 pKa = 11.84APTDD120 pKa = 2.99VGVNSWTCLVIHH132 pKa = 6.75LPFLVHH138 pKa = 6.97RR139 pKa = 11.84DD140 pKa = 3.37VVLRR144 pKa = 11.84WSSANTYY151 pKa = 9.63ADD153 pKa = 3.39SVIYY157 pKa = 10.31SVIYY161 pKa = 10.38NILIDD166 pKa = 4.64PYY168 pKa = 11.05QPTAEE173 pKa = 4.24STGVAIYY180 pKa = 9.8PRR182 pKa = 11.84FDD184 pKa = 3.37SASNLPGSIAEE195 pKa = 4.14FAGIEE200 pKa = 4.03YY201 pKa = 10.65SLLASSSLTPALLTAPGNFSNLFKK225 pKa = 10.74DD226 pKa = 3.96IRR228 pKa = 11.84RR229 pKa = 11.84TCAGYY234 pKa = 7.61TTDD237 pKa = 4.93LDD239 pKa = 4.17ASALYY244 pKa = 9.78NQGRR248 pKa = 11.84VVSGQWTPSISEE260 pKa = 3.99GTIDD264 pKa = 3.94VQTTYY269 pKa = 10.56TPPDD273 pKa = 3.41GTAFTYY279 pKa = 10.88VNNEE283 pKa = 3.61YY284 pKa = 10.78DD285 pKa = 4.08CWDD288 pKa = 3.42MTLPAVSSSTIVSSDD303 pKa = 2.93LFRR306 pKa = 11.84RR307 pKa = 11.84QAEE310 pKa = 4.22AKK312 pKa = 8.82YY313 pKa = 10.12GSYY316 pKa = 9.59MPIRR320 pKa = 11.84LCTPKK325 pKa = 10.16VDD327 pKa = 4.43LVSSSNLRR335 pKa = 11.84QIIARR340 pKa = 11.84APGISAANTNNTAQFEE356 pKa = 4.56ANDD359 pKa = 3.4LHH361 pKa = 7.63LRR363 pKa = 11.84GWSVGVEE370 pKa = 3.5LWMNLDD376 pKa = 3.22PHH378 pKa = 7.77AEE380 pKa = 3.78LRR382 pKa = 11.84LKK384 pKa = 10.6VVEE387 pKa = 4.27NLEE390 pKa = 4.58LDD392 pKa = 3.88PSPSSVYY399 pKa = 10.73SDD401 pKa = 3.68FMTPGYY407 pKa = 9.29PQDD410 pKa = 4.64DD411 pKa = 3.99LALSVIRR418 pKa = 11.84EE419 pKa = 4.09FGRR422 pKa = 11.84RR423 pKa = 11.84SPHH426 pKa = 7.21AYY428 pKa = 9.97DD429 pKa = 4.57ADD431 pKa = 4.09FNRR434 pKa = 11.84LNKK437 pKa = 10.41LGTNIISGLGNVLSTLGIPILSSVAKK463 pKa = 9.67PISNLIVDD471 pKa = 3.72KK472 pKa = 10.56WGYY475 pKa = 10.09KK476 pKa = 9.65PSTYY480 pKa = 10.74SQDD483 pKa = 3.76APSLDD488 pKa = 3.47

Molecular weight:
53.63 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KGX6|A0A1L3KGX6_9VIRU Capsid protein OS=Hubei noda-like virus 9 OX=1922989 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 9.78YY3 pKa = 8.46QHH5 pKa = 6.41EE6 pKa = 4.22FLVRR10 pKa = 11.84FTNIAYY16 pKa = 9.58IIGCLASFTHH26 pKa = 7.61RR27 pKa = 11.84YY28 pKa = 8.78RR29 pKa = 11.84VIFRR33 pKa = 11.84NKK35 pKa = 9.84SAGKK39 pKa = 9.91DD40 pKa = 3.47FQTWCRR46 pKa = 11.84DD47 pKa = 2.84NRR49 pKa = 11.84FRR51 pKa = 11.84LCCYY55 pKa = 8.78WDD57 pKa = 3.46RR58 pKa = 11.84QFAFMGGYY66 pKa = 9.78CVIFQPIILSLCVFYY81 pKa = 11.43NLFVCFLVFSQVIMEE96 pKa = 4.66IACCMVVSFAMDD108 pKa = 3.29NGLDD112 pKa = 4.33CIDD115 pKa = 3.68EE116 pKa = 4.11CSYY119 pKa = 11.13FIDD122 pKa = 4.57LFTVEE127 pKa = 5.25HH128 pKa = 6.62FGADD132 pKa = 3.28RR133 pKa = 11.84DD134 pKa = 3.98LRR136 pKa = 11.84SLFDD140 pKa = 3.52IKK142 pKa = 10.74RR143 pKa = 11.84LTSFKK148 pKa = 10.27PSPHH152 pKa = 5.79HH153 pKa = 4.81THH155 pKa = 7.11GISAAEE161 pKa = 3.77RR162 pKa = 11.84TGASRR167 pKa = 11.84TIRR170 pKa = 11.84EE171 pKa = 4.33AIMNAGYY178 pKa = 10.28KK179 pKa = 9.87PYY181 pKa = 10.4AVSAASMDD189 pKa = 3.61QEE191 pKa = 4.32MGLDD195 pKa = 4.11GNRR198 pKa = 11.84SYY200 pKa = 11.79YY201 pKa = 7.8WAKK204 pKa = 10.67DD205 pKa = 3.59FKK207 pKa = 10.68PKK209 pKa = 10.26YY210 pKa = 9.02QADD213 pKa = 4.72EE214 pKa = 4.16ITSDD218 pKa = 3.53HH219 pKa = 7.46AIMMIDD225 pKa = 3.05VDD227 pKa = 4.63YY228 pKa = 11.6YY229 pKa = 11.4CDD231 pKa = 3.47LYY233 pKa = 11.55YY234 pKa = 11.08YY235 pKa = 8.89MCQFRR240 pKa = 11.84PILMYY245 pKa = 9.75TLQPNFVSYY254 pKa = 10.62RR255 pKa = 11.84SKK257 pKa = 11.12DD258 pKa = 3.36YY259 pKa = 11.1AYY261 pKa = 10.55YY262 pKa = 9.43IQNNKK267 pKa = 7.48IHH269 pKa = 6.8YY270 pKa = 8.34DD271 pKa = 3.29VSGGGKK277 pKa = 9.75YY278 pKa = 8.66SHH280 pKa = 6.6EE281 pKa = 4.0VWDD284 pKa = 4.2YY285 pKa = 11.86DD286 pKa = 3.43SDD288 pKa = 4.15TISIVDD294 pKa = 3.59SFGFNCVYY302 pKa = 10.48HH303 pKa = 6.02VVSKK307 pKa = 9.85TIEE310 pKa = 4.18NPDD313 pKa = 3.55CPGSGRR319 pKa = 11.84RR320 pKa = 11.84LVSLVPLRR328 pKa = 11.84RR329 pKa = 11.84VAPFCYY335 pKa = 9.72KK336 pKa = 10.71PPGQTLKK343 pKa = 10.86RR344 pKa = 11.84KK345 pKa = 7.18TYY347 pKa = 9.19TYY349 pKa = 10.24NGVNIIHH356 pKa = 7.2DD357 pKa = 4.62LVAGTVSMCEE367 pKa = 3.54NGTYY371 pKa = 10.53EE372 pKa = 4.35SVQLSTLEE380 pKa = 3.96YY381 pKa = 8.56TALRR385 pKa = 11.84SIAIRR390 pKa = 11.84KK391 pKa = 8.41EE392 pKa = 3.71KK393 pKa = 10.56EE394 pKa = 3.83LFKK397 pKa = 11.35GDD399 pKa = 3.34VQSILRR405 pKa = 11.84RR406 pKa = 11.84RR407 pKa = 11.84YY408 pKa = 9.1EE409 pKa = 3.98VGTAPPYY416 pKa = 10.68KK417 pKa = 9.97EE418 pKa = 5.69FEE420 pKa = 4.29LQTPIIAHH428 pKa = 7.14LLSKK432 pKa = 10.97VLDD435 pKa = 3.95LPINAIATSGIPVKK449 pKa = 10.71RR450 pKa = 11.84EE451 pKa = 3.59ATAYY455 pKa = 7.36TALGPDD461 pKa = 3.54TLNEE465 pKa = 3.86NRR467 pKa = 11.84FPGQLITSQLASASAILPAKK487 pKa = 10.18NNNNSCASVVGRR499 pKa = 11.84LTNVRR504 pKa = 11.84NTVVPSRR511 pKa = 11.84IYY513 pKa = 10.87GSYY516 pKa = 10.81ADD518 pKa = 4.42EE519 pKa = 4.34FSKK522 pKa = 11.1LVVPDD527 pKa = 3.49ALMRR531 pKa = 11.84KK532 pKa = 9.08GMPVDD537 pKa = 4.16LDD539 pKa = 3.38IVKK542 pKa = 9.62EE543 pKa = 4.1HH544 pKa = 6.25QKK546 pKa = 10.77RR547 pKa = 11.84PLQKK551 pKa = 10.61ARR553 pKa = 11.84IQATEE558 pKa = 4.41HH559 pKa = 5.58VQSTDD564 pKa = 3.01PDD566 pKa = 3.44NRR568 pKa = 11.84LTTFIKK574 pKa = 10.64NEE576 pKa = 3.99PYY578 pKa = 10.88ASANHH583 pKa = 6.33PRR585 pKa = 11.84NITTMAPEE593 pKa = 4.07FTLALSAWTYY603 pKa = 11.13AFKK606 pKa = 10.59EE607 pKa = 4.29DD608 pKa = 4.16CLKK611 pKa = 10.71HH612 pKa = 5.77LKK614 pKa = 10.49SYY616 pKa = 11.24GPGKK620 pKa = 8.99TPLQSAKK627 pKa = 10.36LLRR630 pKa = 11.84DD631 pKa = 3.14ICQNGAVLTDD641 pKa = 3.11YY642 pKa = 10.97TRR644 pKa = 11.84FDD646 pKa = 3.53GTISEE651 pKa = 4.17WLQRR655 pKa = 11.84YY656 pKa = 5.57VVKK659 pKa = 10.61RR660 pKa = 11.84IYY662 pKa = 9.98QRR664 pKa = 11.84WSSDD668 pKa = 3.16PALTNSLFDD677 pKa = 5.26KK678 pKa = 10.5IFKK681 pKa = 10.24QSGVTRR687 pKa = 11.84EE688 pKa = 4.1GKK690 pKa = 10.16RR691 pKa = 11.84FSAFYY696 pKa = 8.45GTRR699 pKa = 11.84SGSPTTTDD707 pKa = 3.08GNTLINMYY715 pKa = 9.04MCYY718 pKa = 9.9CALRR722 pKa = 11.84KK723 pKa = 9.75LGYY726 pKa = 10.38NPTQAFSLLGIYY738 pKa = 10.41AGDD741 pKa = 4.04DD742 pKa = 3.54GVSRR746 pKa = 11.84RR747 pKa = 11.84IPGFDD752 pKa = 3.29EE753 pKa = 3.63QLKK756 pKa = 10.43IVVVDD761 pKa = 4.52LGLSVKK767 pKa = 10.91LEE769 pKa = 4.07VTDD772 pKa = 4.43PGASINYY779 pKa = 9.09LGRR782 pKa = 11.84IFPDD786 pKa = 3.06ILASLSSHH794 pKa = 5.95QDD796 pKa = 2.95MTRR799 pKa = 11.84TLPKK803 pKa = 10.47LHH805 pKa = 6.94ISTNQMVTRR814 pKa = 11.84DD815 pKa = 3.2QAAYY819 pKa = 10.02NKK821 pKa = 10.17AAGYY825 pKa = 10.19YY826 pKa = 8.66VTDD829 pKa = 3.75KK830 pKa = 9.89LTPILGSWAIRR841 pKa = 11.84VMAITNCGNVYY852 pKa = 10.63NEE854 pKa = 4.18TRR856 pKa = 11.84EE857 pKa = 4.07EE858 pKa = 4.04EE859 pKa = 4.13YY860 pKa = 11.11KK861 pKa = 10.6RR862 pKa = 11.84INEE865 pKa = 3.9AWPQEE870 pKa = 4.06VRR872 pKa = 11.84DD873 pKa = 4.08RR874 pKa = 11.84QLLAQSVAEE883 pKa = 4.04QLGLTTPDD891 pKa = 2.72VLAFDD896 pKa = 4.4KK897 pKa = 11.13EE898 pKa = 4.25IMDD901 pKa = 3.61VDD903 pKa = 4.06SLFEE907 pKa = 5.23FPVIWDD913 pKa = 3.58NDD915 pKa = 3.5IEE917 pKa = 4.48DD918 pKa = 5.09KK919 pKa = 11.13INAQIGDD926 pKa = 3.86DD927 pKa = 3.76VVIVSNRR934 pKa = 11.84DD935 pKa = 3.09SVLNEE940 pKa = 3.69TEE942 pKa = 4.42LRR944 pKa = 11.84PNNNAIPTTNTNTRR958 pKa = 11.84RR959 pKa = 11.84RR960 pKa = 11.84DD961 pKa = 3.27RR962 pKa = 11.84RR963 pKa = 11.84EE964 pKa = 3.96RR965 pKa = 11.84NPHH968 pKa = 4.56NQRR971 pKa = 11.84PSTSASDD978 pKa = 3.05NWRR981 pKa = 11.84SDD983 pKa = 3.29TAGTNEE989 pKa = 4.08AGVSRR994 pKa = 11.84SVSEE998 pKa = 4.01NAPGSRR1004 pKa = 11.84NDD1006 pKa = 3.65RR1007 pKa = 11.84SATSPLPRR1015 pKa = 11.84RR1016 pKa = 11.84PRR1018 pKa = 11.84NRR1020 pKa = 11.84RR1021 pKa = 11.84AAILQHH1027 pKa = 6.87SDD1029 pKa = 3.51DD1030 pKa = 4.16TPKK1033 pKa = 9.35PTNAAGLRR1041 pKa = 11.84PTKK1044 pKa = 10.31GRR1046 pKa = 11.84SRR1048 pKa = 11.84PHH1050 pKa = 5.97VPTSRR1055 pKa = 11.84VRR1057 pKa = 11.84SSPVQKK1063 pKa = 10.88

Molecular weight:
120.38 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1551

488

1063

775.5

87.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.286 ± 0.278

1.87 ± 0.355

6.254 ± 0.173

3.997 ± 0.285

3.611 ± 0.526

5.351 ± 0.101

1.741 ± 0.284

5.996 ± 0.143

4.191 ± 0.62

7.801 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.934 ± 0.164

6.319 ± 1.157

5.867 ± 0.725

3.417 ± 0.191

6.899 ± 0.645

8.446 ± 0.999

6.77 ± 0.004

6.383 ± 0.017

1.161 ± 0.152

4.707 ± 0.338

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski