Hubei noda-like virus 9
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGX6|A0A1L3KGX6_9VIRU Capsid protein OS=Hubei noda-like virus 9 OX=1922989 PE=4 SV=1
MM1 pKa = 7.85 ANNNNNAQNTQRR13 pKa = 11.84 GNNSNQPMGTNKK25 pKa = 9.33 VKK27 pKa = 10.36 RR28 pKa = 11.84 SKK30 pKa = 10.49 RR31 pKa = 11.84 RR32 pKa = 11.84 SKK34 pKa = 11.23 QNVNNNAKK42 pKa = 8.78 PTYY45 pKa = 9.81 NPPNQTRR52 pKa = 11.84 SGRR55 pKa = 11.84 MPNPADD61 pKa = 3.42 PRR63 pKa = 11.84 TAIRR67 pKa = 11.84 NLGNTQLGSCAAMKK81 pKa = 10.09 ILHH84 pKa = 6.87 PCNEE88 pKa = 3.77 FDD90 pKa = 4.2 IEE92 pKa = 4.57 VVKK95 pKa = 10.92 IPDD98 pKa = 3.51 GSLPTSVAFEE108 pKa = 3.96 RR109 pKa = 11.84 RR110 pKa = 11.84 DD111 pKa = 3.53 EE112 pKa = 4.21 YY113 pKa = 10.94 EE114 pKa = 3.45 IRR116 pKa = 11.84 APTDD120 pKa = 2.99 VGVNSWTCLVIHH132 pKa = 6.75 LPFLVHH138 pKa = 6.97 RR139 pKa = 11.84 DD140 pKa = 3.37 VVLRR144 pKa = 11.84 WSSANTYY151 pKa = 9.63 ADD153 pKa = 3.39 SVIYY157 pKa = 10.31 SVIYY161 pKa = 10.38 NILIDD166 pKa = 4.64 PYY168 pKa = 11.05 QPTAEE173 pKa = 4.24 STGVAIYY180 pKa = 9.8 PRR182 pKa = 11.84 FDD184 pKa = 3.37 SASNLPGSIAEE195 pKa = 4.14 FAGIEE200 pKa = 4.03 YY201 pKa = 10.65 SLLASSSLTPALLTAPGNFSNLFKK225 pKa = 10.74 DD226 pKa = 3.96 IRR228 pKa = 11.84 RR229 pKa = 11.84 TCAGYY234 pKa = 7.61 TTDD237 pKa = 4.93 LDD239 pKa = 4.17 ASALYY244 pKa = 9.78 NQGRR248 pKa = 11.84 VVSGQWTPSISEE260 pKa = 3.99 GTIDD264 pKa = 3.94 VQTTYY269 pKa = 10.56 TPPDD273 pKa = 3.41 GTAFTYY279 pKa = 10.88 VNNEE283 pKa = 3.61 YY284 pKa = 10.78 DD285 pKa = 4.08 CWDD288 pKa = 3.42 MTLPAVSSSTIVSSDD303 pKa = 2.93 LFRR306 pKa = 11.84 RR307 pKa = 11.84 QAEE310 pKa = 4.22 AKK312 pKa = 8.82 YY313 pKa = 10.12 GSYY316 pKa = 9.59 MPIRR320 pKa = 11.84 LCTPKK325 pKa = 10.16 VDD327 pKa = 4.43 LVSSSNLRR335 pKa = 11.84 QIIARR340 pKa = 11.84 APGISAANTNNTAQFEE356 pKa = 4.56 ANDD359 pKa = 3.4 LHH361 pKa = 7.63 LRR363 pKa = 11.84 GWSVGVEE370 pKa = 3.5 LWMNLDD376 pKa = 3.22 PHH378 pKa = 7.77 AEE380 pKa = 3.78 LRR382 pKa = 11.84 LKK384 pKa = 10.6 VVEE387 pKa = 4.27 NLEE390 pKa = 4.58 LDD392 pKa = 3.88 PSPSSVYY399 pKa = 10.73 SDD401 pKa = 3.68 FMTPGYY407 pKa = 9.29 PQDD410 pKa = 4.64 DD411 pKa = 3.99 LALSVIRR418 pKa = 11.84 EE419 pKa = 4.09 FGRR422 pKa = 11.84 RR423 pKa = 11.84 SPHH426 pKa = 7.21 AYY428 pKa = 9.97 DD429 pKa = 4.57 ADD431 pKa = 4.09 FNRR434 pKa = 11.84 LNKK437 pKa = 10.41 LGTNIISGLGNVLSTLGIPILSSVAKK463 pKa = 9.67 PISNLIVDD471 pKa = 3.72 KK472 pKa = 10.56 WGYY475 pKa = 10.09 KK476 pKa = 9.65 PSTYY480 pKa = 10.74 SQDD483 pKa = 3.76 APSLDD488 pKa = 3.47
Molecular weight: 53.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.744
IPC2_protein 5.817
IPC_protein 5.855
Toseland 6.148
ProMoST 6.008
Dawson 6.109
Bjellqvist 6.122
Wikipedia 6.122
Rodwell 6.084
Grimsley 6.249
Solomon 6.109
Lehninger 6.109
Nozaki 6.364
DTASelect 6.561
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.465
Patrickios 3.999
IPC_peptide 6.135
IPC2_peptide 6.427
IPC2.peptide.svr19 6.474
Protein with the highest isoelectric point:
>tr|A0A1L3KGX6|A0A1L3KGX6_9VIRU Capsid protein OS=Hubei noda-like virus 9 OX=1922989 PE=4 SV=1
MM1 pKa = 7.81 KK2 pKa = 9.78 YY3 pKa = 8.46 QHH5 pKa = 6.41 EE6 pKa = 4.22 FLVRR10 pKa = 11.84 FTNIAYY16 pKa = 9.58 IIGCLASFTHH26 pKa = 7.61 RR27 pKa = 11.84 YY28 pKa = 8.78 RR29 pKa = 11.84 VIFRR33 pKa = 11.84 NKK35 pKa = 9.84 SAGKK39 pKa = 9.91 DD40 pKa = 3.47 FQTWCRR46 pKa = 11.84 DD47 pKa = 2.84 NRR49 pKa = 11.84 FRR51 pKa = 11.84 LCCYY55 pKa = 8.78 WDD57 pKa = 3.46 RR58 pKa = 11.84 QFAFMGGYY66 pKa = 9.78 CVIFQPIILSLCVFYY81 pKa = 11.43 NLFVCFLVFSQVIMEE96 pKa = 4.66 IACCMVVSFAMDD108 pKa = 3.29 NGLDD112 pKa = 4.33 CIDD115 pKa = 3.68 EE116 pKa = 4.11 CSYY119 pKa = 11.13 FIDD122 pKa = 4.57 LFTVEE127 pKa = 5.25 HH128 pKa = 6.62 FGADD132 pKa = 3.28 RR133 pKa = 11.84 DD134 pKa = 3.98 LRR136 pKa = 11.84 SLFDD140 pKa = 3.52 IKK142 pKa = 10.74 RR143 pKa = 11.84 LTSFKK148 pKa = 10.27 PSPHH152 pKa = 5.79 HH153 pKa = 4.81 THH155 pKa = 7.11 GISAAEE161 pKa = 3.77 RR162 pKa = 11.84 TGASRR167 pKa = 11.84 TIRR170 pKa = 11.84 EE171 pKa = 4.33 AIMNAGYY178 pKa = 10.28 KK179 pKa = 9.87 PYY181 pKa = 10.4 AVSAASMDD189 pKa = 3.61 QEE191 pKa = 4.32 MGLDD195 pKa = 4.11 GNRR198 pKa = 11.84 SYY200 pKa = 11.79 YY201 pKa = 7.8 WAKK204 pKa = 10.67 DD205 pKa = 3.59 FKK207 pKa = 10.68 PKK209 pKa = 10.26 YY210 pKa = 9.02 QADD213 pKa = 4.72 EE214 pKa = 4.16 ITSDD218 pKa = 3.53 HH219 pKa = 7.46 AIMMIDD225 pKa = 3.05 VDD227 pKa = 4.63 YY228 pKa = 11.6 YY229 pKa = 11.4 CDD231 pKa = 3.47 LYY233 pKa = 11.55 YY234 pKa = 11.08 YY235 pKa = 8.89 MCQFRR240 pKa = 11.84 PILMYY245 pKa = 9.75 TLQPNFVSYY254 pKa = 10.62 RR255 pKa = 11.84 SKK257 pKa = 11.12 DD258 pKa = 3.36 YY259 pKa = 11.1 AYY261 pKa = 10.55 YY262 pKa = 9.43 IQNNKK267 pKa = 7.48 IHH269 pKa = 6.8 YY270 pKa = 8.34 DD271 pKa = 3.29 VSGGGKK277 pKa = 9.75 YY278 pKa = 8.66 SHH280 pKa = 6.6 EE281 pKa = 4.0 VWDD284 pKa = 4.2 YY285 pKa = 11.86 DD286 pKa = 3.43 SDD288 pKa = 4.15 TISIVDD294 pKa = 3.59 SFGFNCVYY302 pKa = 10.48 HH303 pKa = 6.02 VVSKK307 pKa = 9.85 TIEE310 pKa = 4.18 NPDD313 pKa = 3.55 CPGSGRR319 pKa = 11.84 RR320 pKa = 11.84 LVSLVPLRR328 pKa = 11.84 RR329 pKa = 11.84 VAPFCYY335 pKa = 9.72 KK336 pKa = 10.71 PPGQTLKK343 pKa = 10.86 RR344 pKa = 11.84 KK345 pKa = 7.18 TYY347 pKa = 9.19 TYY349 pKa = 10.24 NGVNIIHH356 pKa = 7.2 DD357 pKa = 4.62 LVAGTVSMCEE367 pKa = 3.54 NGTYY371 pKa = 10.53 EE372 pKa = 4.35 SVQLSTLEE380 pKa = 3.96 YY381 pKa = 8.56 TALRR385 pKa = 11.84 SIAIRR390 pKa = 11.84 KK391 pKa = 8.41 EE392 pKa = 3.71 KK393 pKa = 10.56 EE394 pKa = 3.83 LFKK397 pKa = 11.35 GDD399 pKa = 3.34 VQSILRR405 pKa = 11.84 RR406 pKa = 11.84 RR407 pKa = 11.84 YY408 pKa = 9.1 EE409 pKa = 3.98 VGTAPPYY416 pKa = 10.68 KK417 pKa = 9.97 EE418 pKa = 5.69 FEE420 pKa = 4.29 LQTPIIAHH428 pKa = 7.14 LLSKK432 pKa = 10.97 VLDD435 pKa = 3.95 LPINAIATSGIPVKK449 pKa = 10.71 RR450 pKa = 11.84 EE451 pKa = 3.59 ATAYY455 pKa = 7.36 TALGPDD461 pKa = 3.54 TLNEE465 pKa = 3.86 NRR467 pKa = 11.84 FPGQLITSQLASASAILPAKK487 pKa = 10.18 NNNNSCASVVGRR499 pKa = 11.84 LTNVRR504 pKa = 11.84 NTVVPSRR511 pKa = 11.84 IYY513 pKa = 10.87 GSYY516 pKa = 10.81 ADD518 pKa = 4.42 EE519 pKa = 4.34 FSKK522 pKa = 11.1 LVVPDD527 pKa = 3.49 ALMRR531 pKa = 11.84 KK532 pKa = 9.08 GMPVDD537 pKa = 4.16 LDD539 pKa = 3.38 IVKK542 pKa = 9.62 EE543 pKa = 4.1 HH544 pKa = 6.25 QKK546 pKa = 10.77 RR547 pKa = 11.84 PLQKK551 pKa = 10.61 ARR553 pKa = 11.84 IQATEE558 pKa = 4.41 HH559 pKa = 5.58 VQSTDD564 pKa = 3.01 PDD566 pKa = 3.44 NRR568 pKa = 11.84 LTTFIKK574 pKa = 10.64 NEE576 pKa = 3.99 PYY578 pKa = 10.88 ASANHH583 pKa = 6.33 PRR585 pKa = 11.84 NITTMAPEE593 pKa = 4.07 FTLALSAWTYY603 pKa = 11.13 AFKK606 pKa = 10.59 EE607 pKa = 4.29 DD608 pKa = 4.16 CLKK611 pKa = 10.71 HH612 pKa = 5.77 LKK614 pKa = 10.49 SYY616 pKa = 11.24 GPGKK620 pKa = 8.99 TPLQSAKK627 pKa = 10.36 LLRR630 pKa = 11.84 DD631 pKa = 3.14 ICQNGAVLTDD641 pKa = 3.11 YY642 pKa = 10.97 TRR644 pKa = 11.84 FDD646 pKa = 3.53 GTISEE651 pKa = 4.17 WLQRR655 pKa = 11.84 YY656 pKa = 5.57 VVKK659 pKa = 10.61 RR660 pKa = 11.84 IYY662 pKa = 9.98 QRR664 pKa = 11.84 WSSDD668 pKa = 3.16 PALTNSLFDD677 pKa = 5.26 KK678 pKa = 10.5 IFKK681 pKa = 10.24 QSGVTRR687 pKa = 11.84 EE688 pKa = 4.1 GKK690 pKa = 10.16 RR691 pKa = 11.84 FSAFYY696 pKa = 8.45 GTRR699 pKa = 11.84 SGSPTTTDD707 pKa = 3.08 GNTLINMYY715 pKa = 9.04 MCYY718 pKa = 9.9 CALRR722 pKa = 11.84 KK723 pKa = 9.75 LGYY726 pKa = 10.38 NPTQAFSLLGIYY738 pKa = 10.41 AGDD741 pKa = 4.04 DD742 pKa = 3.54 GVSRR746 pKa = 11.84 RR747 pKa = 11.84 IPGFDD752 pKa = 3.29 EE753 pKa = 3.63 QLKK756 pKa = 10.43 IVVVDD761 pKa = 4.52 LGLSVKK767 pKa = 10.91 LEE769 pKa = 4.07 VTDD772 pKa = 4.43 PGASINYY779 pKa = 9.09 LGRR782 pKa = 11.84 IFPDD786 pKa = 3.06 ILASLSSHH794 pKa = 5.95 QDD796 pKa = 2.95 MTRR799 pKa = 11.84 TLPKK803 pKa = 10.47 LHH805 pKa = 6.94 ISTNQMVTRR814 pKa = 11.84 DD815 pKa = 3.2 QAAYY819 pKa = 10.02 NKK821 pKa = 10.17 AAGYY825 pKa = 10.19 YY826 pKa = 8.66 VTDD829 pKa = 3.75 KK830 pKa = 9.89 LTPILGSWAIRR841 pKa = 11.84 VMAITNCGNVYY852 pKa = 10.63 NEE854 pKa = 4.18 TRR856 pKa = 11.84 EE857 pKa = 4.07 EE858 pKa = 4.04 EE859 pKa = 4.13 YY860 pKa = 11.11 KK861 pKa = 10.6 RR862 pKa = 11.84 INEE865 pKa = 3.9 AWPQEE870 pKa = 4.06 VRR872 pKa = 11.84 DD873 pKa = 4.08 RR874 pKa = 11.84 QLLAQSVAEE883 pKa = 4.04 QLGLTTPDD891 pKa = 2.72 VLAFDD896 pKa = 4.4 KK897 pKa = 11.13 EE898 pKa = 4.25 IMDD901 pKa = 3.61 VDD903 pKa = 4.06 SLFEE907 pKa = 5.23 FPVIWDD913 pKa = 3.58 NDD915 pKa = 3.5 IEE917 pKa = 4.48 DD918 pKa = 5.09 KK919 pKa = 11.13 INAQIGDD926 pKa = 3.86 DD927 pKa = 3.76 VVIVSNRR934 pKa = 11.84 DD935 pKa = 3.09 SVLNEE940 pKa = 3.69 TEE942 pKa = 4.42 LRR944 pKa = 11.84 PNNNAIPTTNTNTRR958 pKa = 11.84 RR959 pKa = 11.84 RR960 pKa = 11.84 DD961 pKa = 3.27 RR962 pKa = 11.84 RR963 pKa = 11.84 EE964 pKa = 3.96 RR965 pKa = 11.84 NPHH968 pKa = 4.56 NQRR971 pKa = 11.84 PSTSASDD978 pKa = 3.05 NWRR981 pKa = 11.84 SDD983 pKa = 3.29 TAGTNEE989 pKa = 4.08 AGVSRR994 pKa = 11.84 SVSEE998 pKa = 4.01 NAPGSRR1004 pKa = 11.84 NDD1006 pKa = 3.65 RR1007 pKa = 11.84 SATSPLPRR1015 pKa = 11.84 RR1016 pKa = 11.84 PRR1018 pKa = 11.84 NRR1020 pKa = 11.84 RR1021 pKa = 11.84 AAILQHH1027 pKa = 6.87 SDD1029 pKa = 3.51 DD1030 pKa = 4.16 TPKK1033 pKa = 9.35 PTNAAGLRR1041 pKa = 11.84 PTKK1044 pKa = 10.31 GRR1046 pKa = 11.84 SRR1048 pKa = 11.84 PHH1050 pKa = 5.97 VPTSRR1055 pKa = 11.84 VRR1057 pKa = 11.84 SSPVQKK1063 pKa = 10.88
Molecular weight: 120.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.67
IPC2_protein 7.951
IPC_protein 7.732
Toseland 7.366
ProMoST 8.361
Dawson 8.463
Bjellqvist 8.799
Wikipedia 8.419
Rodwell 8.507
Grimsley 7.41
Solomon 8.507
Lehninger 8.507
Nozaki 8.902
DTASelect 8.536
Thurlkill 8.58
EMBOSS 8.653
Sillero 8.887
Patrickios 4.151
IPC_peptide 8.492
IPC2_peptide 7.761
IPC2.peptide.svr19 7.985
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1551
488
1063
775.5
87.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.286 ± 0.278
1.87 ± 0.355
6.254 ± 0.173
3.997 ± 0.285
3.611 ± 0.526
5.351 ± 0.101
1.741 ± 0.284
5.996 ± 0.143
4.191 ± 0.62
7.801 ± 0.333
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.934 ± 0.164
6.319 ± 1.157
5.867 ± 0.725
3.417 ± 0.191
6.899 ± 0.645
8.446 ± 0.999
6.77 ± 0.004
6.383 ± 0.017
1.161 ± 0.152
4.707 ± 0.338
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here