Streptococcus phage IPP32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SB98|A0A1S5SB98_9CAUD Uncharacterized protein OS=Streptococcus phage IPP32 OX=1916172 GN=IPP32_00052 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 10.42SDD5 pKa = 3.89LGPYY9 pKa = 9.9LDD11 pKa = 4.98AVTNEE16 pKa = 4.42DD17 pKa = 3.65GTLLICKK24 pKa = 8.59TEE26 pKa = 3.77QGAYY30 pKa = 9.48IGDD33 pKa = 4.28FNPSCDD39 pKa = 3.67EE40 pKa = 3.84EE41 pKa = 5.78DD42 pKa = 3.78FVLTYY47 pKa = 11.02EE48 pKa = 4.44DD49 pKa = 3.86VSVSLSYY56 pKa = 11.13AQVLSATLLKK66 pKa = 10.84VV67 pKa = 3.17

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SB52|A0A1S5SB52_9CAUD Uncharacterized protein OS=Streptococcus phage IPP32 OX=1916172 GN=IPP32_00002 PE=4 SV=1
MM1 pKa = 7.02YY2 pKa = 10.49RR3 pKa = 11.84LVYY6 pKa = 9.87EE7 pKa = 4.72SKK9 pKa = 10.69AQKK12 pKa = 9.04QLKK15 pKa = 9.59KK16 pKa = 10.57LDD18 pKa = 3.27GATRR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 9.61IISWMTKK31 pKa = 10.21NVDD34 pKa = 3.47NTSNPYY40 pKa = 9.63QHH42 pKa = 7.13AKK44 pKa = 9.59LLKK47 pKa = 9.25GNLSGYY53 pKa = 9.33CRR55 pKa = 11.84YY56 pKa = 9.89RR57 pKa = 11.84VGDD60 pKa = 3.49YY61 pKa = 10.78RR62 pKa = 11.84IICDD66 pKa = 3.4IQDD69 pKa = 3.66DD70 pKa = 4.41KK71 pKa = 11.83LVVLAVDD78 pKa = 3.61IAHH81 pKa = 6.5RR82 pKa = 11.84RR83 pKa = 11.84EE84 pKa = 4.15VYY86 pKa = 10.09KK87 pKa = 11.06

Molecular weight: 10.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13183

34

2403

206.0

23.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.07 ± 0.513

0.66 ± 0.099

6.19 ± 0.299

8.003 ± 0.449

3.853 ± 0.241

6.159 ± 0.544

1.441 ± 0.164

6.273 ± 0.304

8.883 ± 0.389

8.245 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.257

5.439 ± 0.209

2.655 ± 0.212

3.952 ± 0.2

4.589 ± 0.291

6.22 ± 0.351

6.357 ± 0.43

6.311 ± 0.285

1.381 ± 0.145

3.876 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski