Phytophthora cactorum
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23398 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A329RW33|A0A329RW33_9STRA Uncharacterized protein OS=Phytophthora cactorum OX=29920 GN=PC110_g14849 PE=4 SV=1
MM1 pKa = 7.77 KK2 pKa = 10.45 SPSSLVLLATAATFAFADD20 pKa = 3.49 AGGIAAEE27 pKa = 4.45 YY28 pKa = 8.03 FTGDD32 pKa = 2.89 GTAYY36 pKa = 9.79 TLGDD40 pKa = 3.74 TSSGNCNMMSALNFATTDD58 pKa = 3.58 YY59 pKa = 11.49 AALNNDD65 pKa = 3.04 QWSGLQNCGRR75 pKa = 11.84 CAEE78 pKa = 4.27 VSCADD83 pKa = 4.19 SRR85 pKa = 11.84 CSDD88 pKa = 3.22 QTKK91 pKa = 10.46 SVVVQILDD99 pKa = 3.38 RR100 pKa = 11.84 CPEE103 pKa = 4.23 CKK105 pKa = 10.06 QGDD108 pKa = 4.41 LDD110 pKa = 4.8 LSPSVFKK117 pKa = 10.34 TLTGSHH123 pKa = 7.3 PSRR126 pKa = 11.84 YY127 pKa = 7.04 TIKK130 pKa = 10.03 WKK132 pKa = 10.51 FVDD135 pKa = 4.75 CPVAGNVNYY144 pKa = 10.11 CLKK147 pKa = 10.76 GGSNNYY153 pKa = 5.91 WTAIQPTNVATGVKK167 pKa = 9.46 SLKK170 pKa = 10.43 INGQATKK177 pKa = 9.46 MLDD180 pKa = 2.98 GAYY183 pKa = 10.49 YY184 pKa = 10.59 YY185 pKa = 11.17 LLDD188 pKa = 4.53 GAGTSQTDD196 pKa = 3.67 LSSVSVSLTDD206 pKa = 3.33 INGVSITDD214 pKa = 3.84 KK215 pKa = 10.81 VSLSAGSCAEE225 pKa = 4.29 GSHH228 pKa = 5.46 QFPSGGSPSKK238 pKa = 10.62 PSTNTSTPTGAPKK251 pKa = 9.11 PTTATPTMAPTKK263 pKa = 10.48 VPTVTTKK270 pKa = 10.14 ATTPTTPTPSQEE282 pKa = 4.25 QQTPAPVPTTSTPMTDD298 pKa = 3.41 TPASTTPTSTTQSGSGDD315 pKa = 3.85 DD316 pKa = 3.98 ASQTAQQDD324 pKa = 3.46 LGSASRR330 pKa = 11.84 NDD332 pKa = 4.08 DD333 pKa = 3.41 ATQSSPQASSSGNGDD348 pKa = 3.32 SPEE351 pKa = 3.85 QSIEE355 pKa = 3.79 QDD357 pKa = 3.23 PGSEE361 pKa = 4.23 SNDD364 pKa = 3.18 VEE366 pKa = 4.35 TQAPITQQSSSSSEE380 pKa = 3.74 GDD382 pKa = 3.06 EE383 pKa = 4.33 TQQQSSTDD391 pKa = 3.7 GEE393 pKa = 4.72 TSTEE397 pKa = 4.21 SPDD400 pKa = 3.45 VPATVAPSTPFSSASQQSGSGVDD423 pKa = 3.79 SEE425 pKa = 5.17 TEE427 pKa = 3.9 ASSSASTYY435 pKa = 8.96 MDD437 pKa = 4.46 CGSSSHH443 pKa = 6.99 PSEE446 pKa = 4.09 QTPSSSNNDD455 pKa = 3.21 NPSQEE460 pKa = 4.36 SQQSAEE466 pKa = 3.82 QDD468 pKa = 3.68 LNSASVDD475 pKa = 3.43 GNTDD479 pKa = 2.61 NSTPIQQSGSTSDD492 pKa = 3.48 NDD494 pKa = 3.7 GTQVTQQDD502 pKa = 4.07 PDD504 pKa = 3.92 SVDD507 pKa = 4.21 SEE509 pKa = 4.68 DD510 pKa = 3.63 STVDD514 pKa = 3.34 NASSAVGADD523 pKa = 2.84 VDD525 pKa = 4.22 MVISQSGSADD535 pKa = 3.46 QNDD538 pKa = 4.34 EE539 pKa = 4.28 NPVQQTSSDD548 pKa = 3.5 VGADD552 pKa = 3.51 TDD554 pKa = 4.63 SEE556 pKa = 4.72 TPEE559 pKa = 3.82 QTSNEE564 pKa = 4.21 DD565 pKa = 3.81 GEE567 pKa = 4.93 TEE569 pKa = 4.26 TPQQDD574 pKa = 3.22 SSSANTEE581 pKa = 3.85 TTEE584 pKa = 4.24 IPSSTGTANCPMM596 pKa = 5.11
Molecular weight: 61.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.617
IPC_protein 3.656
Toseland 3.427
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.605
Rodwell 3.478
Grimsley 3.338
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.783
Patrickios 1.329
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A329S3I7|A0A329S3I7_9STRA Dymeclin OS=Phytophthora cactorum OX=29920 GN=PC110_g12338 PE=3 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.19 RR3 pKa = 11.84 WILPTLKK10 pKa = 10.29 LARR13 pKa = 11.84 VRR15 pKa = 11.84 TLPVVLNKK23 pKa = 9.78 IKK25 pKa = 9.81 KK26 pKa = 8.41 TITSFKK32 pKa = 10.65 KK33 pKa = 10.45 SLQQLSTNSPQRR45 pKa = 11.84 MFRR48 pKa = 11.84 SSLKK52 pKa = 10.44 KK53 pKa = 10.38
Molecular weight: 6.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 10.935
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.618
Grimsley 12.676
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.34
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.973
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23095
303
23398
9185206
24
10474
392.6
43.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.859 ± 0.015
1.621 ± 0.008
5.698 ± 0.011
6.655 ± 0.023
3.711 ± 0.011
6.046 ± 0.019
2.315 ± 0.008
4.14 ± 0.012
5.168 ± 0.017
9.335 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.477 ± 0.008
3.582 ± 0.009
4.7 ± 0.014
4.226 ± 0.014
6.22 ± 0.02
8.289 ± 0.021
6.018 ± 0.018
7.121 ± 0.016
1.209 ± 0.005
2.604 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here