Bacillus taeanensis
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4392 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A366XW07|A0A366XW07_9BACI Iron ABC transporter permease OS=Bacillus taeanensis OX=273032 GN=btuC PE=3 SV=1
MM1 pKa = 7.64 AKK3 pKa = 8.85 YY4 pKa = 9.04 TIVDD8 pKa = 3.65 KK9 pKa = 8.27 EE10 pKa = 4.35 TCIACGACGAAAPDD24 pKa = 3.33 IYY26 pKa = 10.99 DD27 pKa = 3.84 YY28 pKa = 11.45 DD29 pKa = 4.3 DD30 pKa = 3.75 EE31 pKa = 7.04 GIAFVTLDD39 pKa = 3.69 DD40 pKa = 3.75 NKK42 pKa = 11.27 GIVEE46 pKa = 4.28 VPDD49 pKa = 3.55 ILHH52 pKa = 7.13 EE53 pKa = 4.54 DD54 pKa = 3.56 MEE56 pKa = 5.05 DD57 pKa = 3.53 AFEE60 pKa = 4.47 GCPTDD65 pKa = 3.57 SIKK68 pKa = 10.55 IAEE71 pKa = 4.35 EE72 pKa = 4.15 PFDD75 pKa = 4.62 GDD77 pKa = 3.61 ALKK80 pKa = 11.02 FEE82 pKa = 4.68
Molecular weight: 8.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.668
IPC_protein 3.643
Toseland 3.427
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.478
Grimsley 3.35
Solomon 3.617
Lehninger 3.579
Nozaki 3.77
DTASelect 3.986
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.77
Patrickios 0.846
IPC_peptide 3.617
IPC2_peptide 3.745
IPC2.peptide.svr19 3.731
Protein with the highest isoelectric point:
>tr|A0A366XS04|A0A366XS04_9BACI DUF420 domain-containing protein OS=Bacillus taeanensis OX=273032 GN=DS031_13005 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 8.74 PTFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.22 KK14 pKa = 8.69 VHH16 pKa = 5.46 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.49 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.05 GRR39 pKa = 11.84 KK40 pKa = 8.7 VLSAA44 pKa = 4.05
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4392
0
4392
1266379
25
2082
288.3
32.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.019 ± 0.037
0.763 ± 0.011
4.659 ± 0.033
7.832 ± 0.047
4.599 ± 0.036
6.812 ± 0.038
2.195 ± 0.019
7.824 ± 0.04
7.206 ± 0.036
9.87 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.711 ± 0.016
4.561 ± 0.026
3.585 ± 0.019
3.684 ± 0.024
3.861 ± 0.029
5.95 ± 0.026
5.238 ± 0.025
7.019 ± 0.027
1.042 ± 0.015
3.57 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here