Streptomyces phage Caelum

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Arequatrovirus; unclassified Arequatrovirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W0D0|A0A481W0D0_9CAUD Endolysin OS=Streptomyces phage Caelum OX=2530160 GN=27 PE=4 SV=1
MM1 pKa = 7.13NLAVIRR7 pKa = 11.84LHH9 pKa = 7.46IEE11 pKa = 3.27NAYY14 pKa = 10.52ADD16 pKa = 3.73GVEE19 pKa = 4.2IDD21 pKa = 3.96TEE23 pKa = 4.56SVITVPLPYY32 pKa = 9.83PDD34 pKa = 5.29DD35 pKa = 4.89LSEE38 pKa = 4.13QTDD41 pKa = 3.28WEE43 pKa = 4.47YY44 pKa = 11.54EE45 pKa = 4.17HH46 pKa = 7.2IFPEE50 pKa = 4.48TGAGRR55 pKa = 11.84EE56 pKa = 3.75GDD58 pKa = 3.1AVYY61 pKa = 10.01EE62 pKa = 4.24VEE64 pKa = 4.49VVEE67 pKa = 4.98SSAPEE72 pKa = 3.83LLGKK76 pKa = 8.48TFEE79 pKa = 4.27FGYY82 pKa = 11.17

Molecular weight: 9.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W0K0|A0A481W0K0_9CAUD Uncharacterized protein OS=Streptomyces phage Caelum OX=2530160 GN=48 PE=4 SV=1
MM1 pKa = 7.27QVTQAEE7 pKa = 4.48TVEE10 pKa = 4.46RR11 pKa = 11.84RR12 pKa = 11.84SGWGGSRR19 pKa = 11.84LLMMAAMSVKK29 pKa = 9.97VV30 pKa = 3.37

Molecular weight: 3.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

15391

30

1331

183.2

19.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.689 ± 0.352

0.773 ± 0.13

6.387 ± 0.269

6.66 ± 0.424

2.93 ± 0.171

8.362 ± 0.287

2.073 ± 0.197

4.171 ± 0.221

4.646 ± 0.321

8.472 ± 0.383

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.131

2.787 ± 0.163

4.769 ± 0.243

3.216 ± 0.156

6.231 ± 0.393

6.172 ± 0.26

6.673 ± 0.298

7.309 ± 0.189

1.754 ± 0.122

2.755 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski