Holdemanella biformis DSM 3989

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Erysipelotrichaceae; Holdemanella; Holdemanella biformis

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7CBJ1|B7CBJ1_9FIRM Putative selenium-dependent hydroxylase accessory protein YqeC OS=Holdemanella biformis DSM 3989 OX=518637 GN=yqeC PE=4 SV=1
MM1 pKa = 7.28IKK3 pKa = 10.44VYY5 pKa = 10.52TEE7 pKa = 3.65VLGFNNLMYY16 pKa = 10.52RR17 pKa = 11.84YY18 pKa = 8.34MSLPNSLSLADD29 pKa = 3.73LAYY32 pKa = 9.05YY33 pKa = 9.42TLASYY38 pKa = 10.91LSEE41 pKa = 4.28EE42 pKa = 4.26YY43 pKa = 10.88ADD45 pKa = 5.63FVIEE49 pKa = 4.46SNGIPFACPNCEE61 pKa = 3.68DD62 pKa = 3.66EE63 pKa = 5.07QFNKK67 pKa = 10.23LPCASLIQPDD77 pKa = 4.47FKK79 pKa = 11.33EE80 pKa = 4.19GDD82 pKa = 3.88TFKK85 pKa = 10.44MVYY88 pKa = 10.1DD89 pKa = 3.89LEE91 pKa = 4.55EE92 pKa = 4.21PFEE95 pKa = 4.37IEE97 pKa = 3.82IQVEE101 pKa = 4.16KK102 pKa = 11.0VDD104 pKa = 4.96DD105 pKa = 3.92IEE107 pKa = 4.77EE108 pKa = 4.17EE109 pKa = 4.19SSVINGRR116 pKa = 11.84GFAIWDD122 pKa = 3.72GFKK125 pKa = 10.63EE126 pKa = 4.22FQDD129 pKa = 3.97YY130 pKa = 10.65YY131 pKa = 7.73YY132 pKa = 11.31QSMEE136 pKa = 4.01LFKK139 pKa = 10.83EE140 pKa = 4.16YY141 pKa = 11.06VKK143 pKa = 11.24ANDD146 pKa = 3.74MNLSDD151 pKa = 4.64FPVDD155 pKa = 3.41EE156 pKa = 5.19DD157 pKa = 4.97LDD159 pKa = 4.5IIEE162 pKa = 4.57LNKK165 pKa = 10.04VLEE168 pKa = 4.67DD169 pKa = 3.5NFEE172 pKa = 4.11EE173 pKa = 4.76LKK175 pKa = 10.88DD176 pKa = 3.87VYY178 pKa = 10.74EE179 pKa = 4.96GEE181 pKa = 4.23

Molecular weight: 21.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7CCJ7|B7CCJ7_9FIRM Glycerol-3-phosphate cytidylyltransferase OS=Holdemanella biformis DSM 3989 OX=518637 GN=tagD PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.95RR10 pKa = 11.84KK11 pKa = 7.81HH12 pKa = 5.11QKK14 pKa = 8.48VHH16 pKa = 5.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2528

0

2528

730521

33

2587

289.0

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.922 ± 0.055

1.657 ± 0.022

6.044 ± 0.042

6.969 ± 0.045

4.477 ± 0.041

5.885 ± 0.052

2.013 ± 0.023

7.941 ± 0.053

8.054 ± 0.046

9.151 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.17 ± 0.026

5.378 ± 0.042

2.848 ± 0.023

3.496 ± 0.03

3.49 ± 0.034

6.168 ± 0.039

5.169 ± 0.039

6.814 ± 0.043

0.817 ± 0.016

4.537 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski